Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Campagnoli, C.
Right arrow Articles by Fisk, N. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Campagnoli, C.
Right arrow Articles by Fisk, N. M.
Related Collections
Right arrow Hematopoiesis
Social Bookmarking
 Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

Blood, 15 October 2001, Vol. 98, No. 8, pp. 2396-2402

HEMATOPOIESIS

Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow

Cesare Campagnoli, Irene A. G. Roberts, Sailesh Kumar, Phillip R. Bennett, Ilaria Bellantuono, and Nicholas M. Fisk

From the Department of Maternal and Fetal Medicine, Institute of Reproductive and Developmental Biology; and the Departments of Haematology and Immunology, Imperial College School of Medicine, London, United Kingdom.

Human mesenchymal stem/progenitor cells (MSCs) have been identified in adult bone marrow, but little is known about their presence during fetal life. MSCs were isolated and characterized in first-trimester fetal blood, liver, and bone marrow. When 106 fetal blood nucleated cells (median gestational age, 10+2 weeks [10 weeks, 2 days]) were cultured in 10% fetal bovine serum, the mean number (± SEM) of adherent fibroblastlike colonies was 8.2 ± 0.6/106 nucleated cells (69.6 ± 10/µL fetal blood). Frequency declined with advancing gestation. Fetal blood MSCs could be expanded for at least 20 passages with a mean cumulative population doubling of 50.3 ± 4.5. In their undifferentiated state, fetal blood MSCs were CD29+, CD44+, SH2+, SH3+, and SH4+; produced prolyl-4-hydroxylase, alpha -smooth muscle actin, fibronectin, laminin, and vimentin; and were CD45-, CD34-, CD14-, CD68-, vWF-, and HLA-DR-. Fetal blood MSCs cultured in adipogenic, osteogenic, or chondrogenic media differentiated, respectively, into adipocytes, osteocytes, and chondrocytes. Fetal blood MSCs supported the proliferation and differentiation of cord blood CD34+ cells in long-term culture. MSCs were also detected in first-trimester fetal liver (11.3 ± 2.0/106 nucleated cells) and bone marrow (12.6 ± 3.6/106 nucleated cells). Their morphology, growth kinetics, and immunophenotype were comparable to those of fetal blood-derived MSCs and similarly differentiated along adipogenic, osteogenic, and chondrogenic lineages, even after sorting and expansion of a single mesenchymal cell. MSCs similar to those derived from adult bone marrow, fetal liver, and fetal bone marrow circulate in first-trimester human blood and may provide novel targets for in utero cellular and gene therapy.

© 2001 by The American Society of Hematology.
 

Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Exp. Biol. Med.Home page
C. K. Rebelatto, A. M. Aguiar, M. P. Moretao, A. C. Senegaglia, P. Hansen, F. Barchiki, J. Oliveira, J. Martins, C. Kuligovski, F. Mansur, et al.
Dissimilar Differentiation of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, and Adipose Tissue
Experimental Biology and Medicine, July 1, 2008; 233(7): 901 - 913.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
P. J. Mishra, P. J. Mishra, R. Humeniuk, D. J. Medina, G. Alexe, J. P. Mesirov, S. Ganesan, J. W. Glod, and D. Banerjee
Carcinoma-Associated Fibroblast-Like Differentiation of Human Mesenchymal Stem Cells
Cancer Res., June 1, 2008; 68(11): 4331 - 4339.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
G. Lazennec and C. Jorgensen
Concise Review: Adult Multipotent Stromal Cells and Cancer: Risk or Benefit?
Stem Cells, June 1, 2008; 26(6): 1387 - 1394.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
I. A. Potapova, P. R. Brink, I. S. Cohen, and S. V. Doronin
Culturing of Human Mesenchymal Stem Cells as Three-dimensional Aggregates Induces Functional Expression of CXCR4 That Regulates Adhesion to Endothelial Cells
J. Biol. Chem., May 9, 2008; 283(19): 13100 - 13107.
[Abstract] [Full Text] [PDF]


Home page
J. Thorac. Cardiovasc. Surg.Home page
X. Hu, S. P. Yu, J. L. Fraser, Z. Lu, M. E. Ogle, J.-A. Wang, and L. Wei
Transplantation of hypoxia-preconditioned mesenchymal stem cells improves infarcted heart function via enhanced survival of implanted cells and angiogenesis.
J. Thorac. Cardiovasc. Surg., April 1, 2008; 135(4): 799 - 808.
[Abstract] [Full Text] [PDF]


Home page
haematolHome page
N. Sessarego, A. Parodi, M. Podesta, F. Benvenuto, M. Mogni, V. Raviolo, M. Lituania, A. Kunkl, G. Ferlazzo, F. D. Bricarelli, et al.
Multipotent mesenchymal stromal cells from amniotic fluid: solid perspectives for clinical application
Haematologica, March 1, 2008; 93(3): 339 - 346.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
D. L. Troyer and M. L. Weiss
Concise Review: Wharton's Jelly-Derived Cells Are a Primitive Stromal Cell Population
Stem Cells, March 1, 2008; 26(3): 591 - 599.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
X.-Y. Wang, Y. Lan, W.-Y. He, L. Zhang, H.-Y. Yao, C.-M. Hou, Y. Tong, Y.-L. Liu, G. Yang, X.-D. Liu, et al.
Identification of mesenchymal stem cells in aorta-gonad-mesonephros and yolk sac of human embryos
Blood, February 15, 2008; 111(4): 2436 - 2443.
[Abstract] [Full Text] [PDF]


Home page
Rheumatology (Oxford)Home page
E. Jones and D. McGonagle
Human bone marrow mesenchymal stem cells in vivo
Rheumatology, February 1, 2008; 47(2): 126 - 131.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
P. V. Guillot, O. Abass, J. H. D. Bassett, S. J. Shefelbine, G. Bou-Gharios, J. Chan, H. Kurata, G. R. Williams, J. Polak, and N. M. Fisk
Intrauterine transplantation of human fetal mesenchymal stem cells from first-trimester blood repairs bone and reduces fractures in osteogenesis imperfecta mice
Blood, February 1, 2008; 111(3): 1717 - 1725.
[Abstract] [Full Text] [PDF]


Home page
Ann. N. Y. Acad. Sci.Home page
N. ZAIDI and A. J. NIXON
Stem Cell Therapy in Bone Repair and Regeneration
Ann. N.Y. Acad. Sci., November 1, 2007; 1117(1): 62 - 72.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
G. Chamberlain, J. Fox, B. Ashton, and J. Middleton
Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing
Stem Cells, November 1, 2007; 25(11): 2739 - 2749.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
D. G. Phinney and D. J. Prockop
Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair Current Views
Stem Cells, November 1, 2007; 25(11): 2896 - 2902.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
S. Jones, N. Horwood, A. Cope, and F. Dazzi
The Antiproliferative Effect of Mesenchymal Stem Cells Is a Fundamental Property Shared by All Stromal Cells
J. Immunol., September 1, 2007; 179(5): 2824 - 2831.
[Abstract] [Full Text] [PDF]


Home page
Reproductive SciencesHome page
C. E. Gargett
Review Article: Stem Cells in Human Reproduction
Reproductive Sciences, July 1, 2007; 14(5): 405 - 424.
[Abstract] [PDF]


Home page
Stem CellsHome page
S. A. Kuznetsov, M. H. Mankani, A. I. Leet, N. Ziran, S. Gronthos, and P. G. Robey
Circulating Connective Tissue Precursors: Extreme Rarity in Humans and Chondrogenic Potential in Guinea Pigs
Stem Cells, July 1, 2007; 25(7): 1830 - 1839.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
Y. Zhao and S. Ding
A high-throughput siRNA library screen identifies osteogenic suppressors in human mesenchymal stem cells
PNAS, June 5, 2007; 104(23): 9673 - 9678.
[Abstract] [Full Text] [PDF]


Home page
Physiol. GenomicsHome page
S. Chateauvieux, J.-L. Ichante, B. Delorme, V. Frouin, G. Pietu, A. Langonne, N. Gallay, L. Sensebe, M. T. Martin, K. A. Moore, et al.
Molecular profile of mouse stromal mesenchymal stem cells
Physiol Genomics, April 24, 2007; 29(2): 128 - 138.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
J. Chan, S. N. Waddington, K. O'Donoghue, H. Kurata, P. V. Guillot, C. Gotherstrom, M. Themis, J. E. Morgan, and N. M. Fisk
Widespread Distribution and Muscle Differentiation of Human Fetal Mesenchymal Stem Cells After Intrauterine Transplantation in Dystrophic mdx Mouse
Stem Cells, April 1, 2007; 25(4): 875 - 884.
[Abstract] [Full Text] [PDF]


Home page
Eur. J. Cardiothorac. Surg.Home page
X. Hu, J. Wang, J. Chen, R. Luo, A. He, X. Xie, and J. Li
Optimal temporal delivery of bone marrow mesenchymal stem cells in rats with myocardial infarction
Eur. J. Cardiothorac. Surg., March 1, 2007; 31(3): 438 - 443.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
P. V. Guillot, C. Gotherstrom, J. Chan, H. Kurata, and N. M. Fisk
Human First-Trimester Fetal MSC Express Pluripotency Markers and Grow Faster and Have Longer Telomeres Than Adult MSC
Stem Cells, March 1, 2007; 25(3): 646 - 654.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
L. G. Menon, S. Picinich, R. Koneru, H. Gao, S. Y. Lin, M. Koneru, P. Mayer-Kuckuk, J. Glod, and D. Banerjee
Differential Gene Expression Associated with Migration of Mesenchymal Stem Cells to Conditioned Medium from Tumor Cells or Bone Marrow Cells
Stem Cells, February 1, 2007; 25(2): 520 - 528.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
Q. He, C. Wan, and G. Li
Concise Review: Multipotent Mesenchymal Stromal Cells in Blood
Stem Cells, January 1, 2007; 25(1): 69 - 77.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
G. Y. Rochefort, B. Delorme, A. Lopez, O. Herault, P. Bonnet, P. Charbord, V. Eder, and J. Domenech
Multipotential Mesenchymal Stem Cells Are Mobilized into Peripheral Blood by Hypoxia
Stem Cells, October 1, 2006; 24(10): 2202 - 2208.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
A. J. Nauta, A. B. Kruisselbrink, E. Lurvink, R. Willemze, and W. E. Fibbe
Mesenchymal Stem Cells Inhibit Generation and Function of Both CD34+-Derived and Monocyte-Derived Dendritic Cells
J. Immunol., August 15, 2006; 177(4): 2080 - 2087.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
J. Chan, K. O'Donoghue, M. Gavina, Y. Torrente, N. Kennea, H. Mehmet, H. Stewart, D. J. Watt, J. E. Morgan, and N. M. Fisk
Galectin-1 Induces Skeletal Muscle Differentiation in Human Fetal Mesenchymal Stem Cells and Increases Muscle Regeneration
Stem Cells, August 1, 2006; 24(8): 1879 - 1891.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
J. Koerner, D. Nesic, J. D. Romero, W. Brehm, P. Mainil-Varlet, and S. P. Grogan
Equine peripheral blood-derived progenitors in comparison to bone marrow-derived mesenchymal stem cells.
Stem Cells, June 1, 2006; 24(6): 1613 - 1619.
[Abstract] [Full Text] [PDF]


Home page
Cardiovasc ResHome page
S. Y. Lim, Y. S. Kim, Y. Ahn, M. H. Jeong, M. H. Hong, S. Y. Joo, K. I. Nam, J. G. Cho, P. M. Kang, and J. C. Park
The effects of mesenchymal stem cells transduced with Akt in a porcine myocardial infarction model
Cardiovasc Res, June 1, 2006; 70(3): 530 - 542.
[Abstract] [Full Text] [PDF]


Home page
Ann. N. Y. Acad. Sci.Home page
J. M. POLAK and A. E. BISHOP
Stem Cells and Tissue Engineering: Past, Present, and Future
Ann. N.Y. Acad. Sci., April 1, 2006; 1068(1): 352 - 366.
[Abstract] [Full Text] [PDF]


Home page
J Bone Joint Surg BrHome page
I. Pountos, E. Jones, C. Tzioupis, D. McGonagle, and P. V. Giannoudis
Growing bone and cartilage: THE ROLE OF MESENCHYMAL STEM CELLS
J Bone Joint Surg Br, April 1, 2006; 88-B(4): 421 - 426.
[Full Text] [PDF]


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. C. Horowitz, Z. Cui, T. A. Moore, T. R. Meier, R. C. Reddy, G. B. Toews, T. J. Standiford, and V. J. Thannickal
Constitutive activation of prosurvival signaling in alveolar mesenchymal cells isolated from patients with nonresolving acute respiratory distress syndrome
Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L415 - L425.
[Abstract] [Full Text] [PDF]


Home page
Exp. Biol. Med.Home page
J. J. Minguell and A. Erices
Mesenchymal Stem Cells and the Treatment of Cardiac Disease
Experimental Biology and Medicine, January 1, 2006; 231(1): 39 - 49.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
S. Knaan-Shanzer, M. J.M. van de Watering, I. van der Velde, M. A.F.V. Goncalves, D. Valerio, and A. A.F. de Vries
Endowing Human Adenovirus Serotype 5 Vectors with Fiber Domains of Species B Greatly Enhances Gene Transfer into Human Mesenchymal Stem Cells
Stem Cells, October 1, 2005; 23(10): 1598 - 1607.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
T. Tondreau, N. Meuleman, A. Delforge, M. Dejeneffe, R. Leroy, M. Massy, C. Mortier, D. Bron, and L. Lagneaux
Mesenchymal Stem Cells Derived from CD133-Positive Cells in Mobilized Peripheral Blood and Cord Blood: Proliferation, Oct4 Expression, and Plasticity
Stem Cells, September 1, 2005; 23(8): 1105 - 1112.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
J. R. Fuchs, D. Hannouche, S. Terada, S. Zand, J. P. Vacanti, and D. O. Fauza
Cartilage Engineering from Ovine Umbilical Cord Blood Mesenchymal Progenitor Cells
Stem Cells, August 1, 2005; 23(7): 958 - 964.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
V. Sordi, M. L. Malosio, F. Marchesi, A. Mercalli, R. Melzi, T. Giordano, N. Belmonte, G. Ferrari, B. E. Leone, F. Bertuzzi, et al.
Bone marrow mesenchymal stem cells express a restricted set of functionally active chemokine receptors capable of promoting migration to pancreatic islets
Blood, July 15, 2005; 106(2): 419 - 427.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
K. Khosrotehrani and D. W. Bianchi
Multi-lineage potential of fetal cells in maternal tissue: a legacy in reverse
J. Cell Sci., April 15, 2005; 118(8): 1559 - 1563.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
G. Cronwright, K. Le Blanc, C. Gotherstrom, P. Darcy, M. Ehnman, and B. Brodin
Cancer/Testis Antigen Expression in Human Mesenchymal Stem Cells: Down-regulation of SSX Impairs Cell Migration and Matrix Metalloproteinase 2 Expression
Cancer Res., March 15, 2005; 65(6): 2207 - 2215.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
J. Chan, K. O'Donoghue, J. de la Fuente, I. A. Roberts, S. Kumar, J. E. Morgan, and N. M. Fisk
Human Fetal Mesenchymal Stem Cells as Vehicles for Gene Delivery
Stem Cells, January 1, 2005; 23(1): 93 - 102.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
R. A. Panepucci, J. L.C. Siufi, W. A. Silva Jr., R. Proto-Siquiera, L. Neder, M. Orellana, V. Rocha, D. T. Covas, and M. A. Zago
Comparison of Gene Expression of Umbilical Cord Vein and Bone Marrow-Derived Mesenchymal Stem Cells
Stem Cells, December 1, 2004; 22(7): 1263 - 1278.
[Abstract] [Full Text] [PDF]


Home page
Stem CellsHome page
P. S. in 't Anker, S. A. Scherjon, C. Kleijburg-van der Keur, G. M.J.S. de Groot-Swings, F. H.J. Claas, W. E. Fibbe, and H. H.H. Kanhai
Isolation of Mesenchymal Stem Cells of Fetal or Maternal Origin from Human Placenta
Stem Cells, December 1, 2004; 22(7): 1338 - 1345.
[Abstract] [Full Text] [PDF]


Home page