SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Campagnoli, C. Articles by Fisk, N. M. Search for Related Content PubMed PubMed Citation Articles by Campagnoli, C. Articles by Fisk, N. M. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 October 2001, Vol. 98, No. 8, pp. 2396-2402 HEMATOPOIESIS Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow Cesare Campagnoli, Irene A. G. Roberts, Sailesh Kumar, Phillip R. Bennett, Ilaria Bellantuono, and Nicholas M. Fisk From the Department of Maternal and Fetal Medicine, Institute of Reproductive and Developmental Biology; and the Departments of Haematology and Immunology, Imperial College School of Medicine, London, United Kingdom. Human mesenchymal stem/progenitor cells (MSCs) have been identified in adult bone marrow, but little is known about their presence during fetal life. MSCs were isolated and characterized in first-trimester fetal blood, liver, and bone marrow. When 106 fetal blood nucleated cells (median gestational age, 10+2 weeks [10 weeks, 2 days]) were cultured in 10% fetal bovine serum, the mean number (± SEM) of adherent fibroblastlike colonies was 8.2 ± 0.6/106 nucleated cells (69.6 ± 10/µL fetal blood). Frequency declined with advancing gestation. Fetal blood MSCs could be expanded for at least 20 passages with a mean cumulative population doubling of 50.3 ± 4.5. In their undifferentiated state, fetal blood MSCs were CD29+, CD44+, SH2+, SH3+, and SH4+; produced prolyl-4-hydroxylase, -smooth muscle actin, fibronectin, laminin, and vimentin; and were CD45, CD34, CD14, CD68, vWF, and HLA-DR. Fetal blood MSCs cultured in adipogenic, osteogenic, or chondrogenic media differentiated, respectively, into adipocytes, osteocytes, and chondrocytes. Fetal blood MSCs supported the proliferation and differentiation of cord blood CD34+ cells in long-term culture. MSCs were also detected in first-trimester fetal liver (11.3 ± 2.0/106 nucleated cells) and bone marrow (12.6 ± 3.6/106 nucleated cells). Their morphology, growth kinetics, and immunophenotype were comparable to those of fetal blood-derived MSCs and similarly differentiated along adipogenic, osteogenic, and chondrogenic lineages, even after sorting and expansion of a single mesenchymal cell. MSCs similar to those derived from adult bone marrow, fetal liver, and fetal bone marrow circulate in first-trimester human blood and may provide novel targets for in utero cellular and gene therapy. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. Mizokami, H. Hisha, S. Okazaki, T. Takaki, X.-L. Wang, C.-Y. Song, Q. Li, J. Kato, N. Hosaka, M. Inaba, et al. Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched amnion-derived mesenchymal stem cells Haematologica, May 1, 2009; 94(5): 618 - 628. [Abstract] [Full Text] [PDF] Y. Kamada, Y. Yoshida, Y. Saji, J. Fukushima, S. Tamura, S. Kiso, and N. Hayashi Transplantation of basic fibroblast growth factor-pretreated adipose tissue-derived stromal cells enhances regression of liver fibrosis in mice Am J Physiol Gastrointest Liver Physiol, February 1, 2009; 296(2): G157 - G167. [Abstract] [Full Text] [PDF] J. Chan, S. Kumar, and N. M. Fisk First trimester embryo-fetoscopic and ultrasound-guided fetal blood sampling for ex vivo viral transduction of cultured human fetal mesenchymal stem cells Hum. Reprod., November 1, 2008; 23(11): 2427 - 2437. [Abstract] [Full Text] [PDF] N. Derval, L. Barandon, P. Dufourcq, L. Leroux, J.-M. D. Lamaziere, D. Daret, T. Couffinhal, and C. Duplaa Epicardial deposition of endothelial progenitor and mesenchymal stem cells in a coated muscle patch after myocardial infarction in a murine model. Eur. J. Cardiothorac. Surg., August 1, 2008; 34(2): 248 - 254. [Abstract] [Full Text] [PDF] C. K. Rebelatto, A. M. Aguiar, M. P. Moretao, A. C. Senegaglia, P. Hansen, F. Barchiki, J. Oliveira, J. Martins, C. Kuligovski, F. Mansur, et al. Dissimilar Differentiation of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, and Adipose Tissue Experimental Biology and Medicine, July 1, 2008; 233(7): 901 - 913. [Abstract] [Full Text] [PDF] P. J. Mishra, P. J. Mishra, R. Humeniuk, D. J. Medina, G. Alexe, J. P. Mesirov, S. Ganesan, J. W. Glod, and D. Banerjee Carcinoma-Associated Fibroblast-Like Differentiation of Human Mesenchymal Stem Cells Cancer Res., June 1, 2008; 68(11): 4331 - 4339. [Abstract] [Full Text] [PDF] I. A. Potapova, P. R. Brink, I. S. Cohen, and S. V. Doronin Culturing of Human Mesenchymal Stem Cells as Three-dimensional Aggregates Induces Functional Expression of CXCR4 That Regulates Adhesion to Endothelial Cells J. Biol. Chem., May 9, 2008; 283(19): 13100 - 13107. [Abstract] [Full Text] [PDF] X. Hu, S. P. Yu, J. L. Fraser, Z. Lu, M. E. Ogle, J.-A. Wang, and L. Wei Transplantation of hypoxia-preconditioned mesenchymal stem cells improves infarcted heart function via enhanced survival of implanted cells and angiogenesis. J. Thorac. Cardiovasc. Surg., April 1, 2008; 135(4): 799 - 808. [Abstract] [Full Text] [PDF] N. Sessarego, A. Parodi, M. Podesta, F. Benvenuto, M. Mogni, V. Raviolo, M. Lituania, A. Kunkl, G. Ferlazzo, F. D. Bricarelli, et al. Multipotent mesenchymal stromal cells from amniotic fluid: solid perspectives for clinical application Haematologica, March 1, 2008; 93(3): 339 - 346. [Abstract] [Full Text] [PDF] X.-Y. Wang, Y. Lan, W.-Y. He, L. Zhang, H.-Y. Yao, C.-M. Hou, Y. Tong, Y.-L. Liu, G. Yang, X.-D. Liu, et al. Identification of mesenchymal stem cells in aorta-gonad-mesonephros and yolk sac of human embryos Blood, February 15, 2008; 111(4): 2436 - 2443. [Abstract] [Full Text] [PDF] E. Jones and D. McGonagle Human bone marrow mesenchymal stem cells in vivo Rheumatology, February 1, 2008; 47(2): 126 - 131. [Abstract] [Full Text] [PDF] P. V. Guillot, O. Abass, J. H. D. Bassett, S. J. Shefelbine, G. Bou-Gharios, J. Chan, H. Kurata, G. R. Williams, J. Polak, and N. M. Fisk Intrauterine transplantation of human fetal mesenchymal stem cells from first-trimester blood repairs bone and reduces fractures in osteogenesis imperfecta mice Blood, February 1, 2008; 111(3): 1717 - 1725. [Abstract] [Full Text] [PDF] S. Jones, N. Horwood, A. Cope, and F. Dazzi The Antiproliferative Effect of Mesenchymal Stem Cells Is a Fundamental Property Shared by All Stromal Cells J. Immunol., September 1, 2007; 179(5): 2824 - 2831. [Abstract] [Full Text] [PDF] C. E. Gargett Review Article: Stem Cells in Human Reproduction Reproductive Sciences, July 1, 2007; 14(5): 405 - 424. [Abstract] [PDF] Y. Zhao and S. Ding A high-throughput siRNA library screen identifies osteogenic suppressors in human mesenchymal stem cells PNAS, June 5, 2007; 104(23): 9673 - 9678. [Abstract] [Full Text] [PDF] S. Chateauvieux, J.-L. Ichante, B. Delorme, V. Frouin, G. Pietu, A. Langonne, N. Gallay, L. Sensebe, M. T. Martin, K. A. Moore, et al. Molecular profile of mouse stromal mesenchymal stem cells Physiol Genomics, April 24, 2007; 29(2): 128 - 138. [Abstract] [Full Text] [PDF] X. Hu, J. Wang, J. Chen, R. Luo, A. He, X. Xie, and J. Li Optimal temporal delivery of bone marrow mesenchymal stem cells in rats with myocardial infarction Eur. J. Cardiothorac. Surg., March 1, 2007; 31(3): 438 - 443. [Abstract] [Full Text] [PDF] A. J. Nauta, A. B. Kruisselbrink, E. Lurvink, R. Willemze, and W. E. Fibbe Mesenchymal Stem Cells Inhibit Generation and Function of Both CD34+-Derived and Monocyte-Derived Dendritic Cells J. Immunol., August 15, 2006; 177(4): 2080 - 2087. [Abstract] [Full Text] [PDF] S. Y. Lim, Y. S. Kim, Y. Ahn, M. H. Jeong, M. H. Hong, S. Y. Joo, K. I. Nam, J. G. Cho, P. M. Kang, and J. C. Park The effects of mesenchymal stem cells transduced with Akt in a porcine myocardial infarction model Cardiovasc Res, June 1, 2006; 70(3): 530 - 542. [Abstract] [Full Text] [PDF] I. Pountos, E. Jones, C. Tzioupis, D. McGonagle, and P. V. Giannoudis Growing bone and cartilage: THE ROLE OF MESENCHYMAL STEM CELLS J Bone Joint Surg Br, April 1, 2006; 88-B(4): 421 - 426. [Full Text] [PDF] J. C. Horowitz, Z. Cui, T. A. Moore, T. R. Meier, R. C. Reddy, G. B. Toews, T. J. Standiford, and V. J. Thannickal Constitutive activation of prosurvival signaling in alveolar mesenchymal cells isolated from patients with nonresolving acute respiratory distress syndrome Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L415 - L425. [Abstract] [Full Text] [PDF] J. J. Minguell and A. Erices Mesenchymal Stem Cells and the Treatment of Cardiac Disease Experimental Biology and Medicine, January 1, 2006; 231(1): 39 - 49. [Abstract] [Full Text] [PDF] V. Sordi, M. L. Malosio, F. Marchesi, A. Mercalli, R. Melzi, T. Giordano, N. Belmonte, G. Ferrari, B. E. Leone, F. Bertuzzi, et al. Bone marrow mesenchymal stem cells express a restricted set of functionally active chemokine receptors capable of promoting migration to pancreatic islets Blood, July 15, 2005; 106(2): 419 - 427. [Abstract] [Full Text] [PDF] K. Khosrotehrani and D. W. Bianchi Multi-lineage potential of fetal cells in maternal tissue: a legacy in reverse J. Cell Sci., April 15, 2005; 118(8): 1559 - 1563. [Abstract] [Full Text] [PDF] G. Cronwright, K. Le Blanc, C. Gotherstrom, P. Darcy, M. Ehnman, and B. Brodin Cancer/Testis Antigen Expression in Human Mesenchymal Stem Cells: Down-regulation of SSX Impairs Cell Migration and Matrix Metalloproteinase 2 Expression Cancer Res., March 15, 2005; 65(6): 2207 - 2215. [Abstract] [Full Text] [PDF] C. H. Parsons, B. Szomju, and D. H. Kedes Susceptibility of human fetal mesencyhmal stem cells to Kaposi sarcoma-associated herpesvirus Blood, November 1, 2004; 104(9): 2736 - 2738. [Abstract] [Full Text] [PDF] H T Hassan and M El-Sheemy Adult bone-marrow stem cells and their potential in medicine J R Soc Med, October 1, 2004; 97(10): 465 - 471. [Full Text] [PDF] M. Abedin, Y. Tintut, and L. L. Demer Mesenchymal Stem Cells and the Artery Wall Circ. Res., October 1, 2004; 95(7): 671 - 676. [Abstract] [Full Text] [PDF] K. Khosrotehrani, K. L. Johnson, D. H. Cha, R. N. Salomon, and D. W. Bianchi Transfer of Fetal Cells With Multilineage Potential to Maternal Tissue JAMA, July 7, 2004; 292(1): 75 - 80. [Abstract] [Full Text] [PDF] W. Xu, X. Zhang, H. Qian, W. Zhu, X. Sun, J. Hu, H. Zhou, and Y. Chen Mesenchymal Stem Cells from Adult Human Bone Marrow Differentiate into a Cardiomyocyte Phenotype In Vitro Experimental Biology and Medicine, July 1, 2004; 229(7): 623 - 631. [Abstract] [Full Text] [PDF] M.-S. Tsai, J.-L. Lee, Y.-J. Chang, and S.-M. Hwang Isolation of human multipotent mesenchymal stem cells from second-trimester amniotic fluid using a novel two-stage culture protocol Hum. Reprod., June 1, 2004; 19(6): 1450 - 1456. [Abstract] [Full Text] [PDF] S. Kumar and A. O'Brien Recent developments in fetal medicine BMJ, April 24, 2004; 328(7446): 1002 - 1006. [Full Text] [PDF] Y. Tintut, Z. Alfonso, T. Saini, K. Radcliff, K. Watson, K. Bostrom, and L. L. Demer Multilineage Potential of Cells From the Artery Wall Circulation, November 18, 2003; 108(20): 2505 - 2510. [Abstract] [Full Text] [PDF] M. Kuwana, Y. Okazaki, H. Kodama, K. Izumi, H. Yasuoka, Y. Ogawa, Y. Kawakami, and Y. Ikeda Human circulating CD14+ monocytes as a source of progenitors that exhibit mesenchymal cell differentiation J. Leukoc. Biol., November 1, 2003; 74(5): 833 - 845. [Abstract] [Full Text] [PDF] P. S. in `t Anker, S. A. Scherjon, C. Kleijburg-van der Keur, W. A. Noort, F. H. J. Claas, R. Willemze, W. E. Fibbe, and H. H. H. Kanhai Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation Blood, August 15, 2003; 102(4): 1548 - 1549. [Full Text] [PDF] K. O'Donoghue, M. Choolani, J. Chan, J. de la Fuente, S. Kumar, C. Campagnoli, P.R. Bennett, I.A.G. Roberts, and N.M. Fisk Identification of fetal mesenchymal stem cells in maternal blood: implications for non-invasive prenatal diagnosis Mol. Hum. Reprod., August 1, 2003; 9(8): 497 - 502. [Abstract] [Full Text] [PDF] J. Chagraoui, A. Lepage-Noll, A. Anjo, G. Uzan, and P. Charbord Fetal liver stroma consists of cells in epithelial-to-mesenchymal transition Blood, April 15, 2003; 101(8): 2973 - 2982. [Abstract] [Full Text] [PDF] H. A. Elicha Gussin, F. Z. Bischoff, R. Hoffman, and S. Elias Endothelial Precursor Cells in the Peripheral Blood of Pregnant Women Reproductive Sciences, November 1, 2002; 9(6): 357 - 361. [Abstract] [PDF] W E Fibbe Mesenchymal stem cells. A potential source for skeletal repair Ann Rheum Dis, November 1, 2002; 61(90002): ii29 - 31. [Full Text] [PDF] T. Ozawa, D. A. G. Mickle, R. D. Weisel, N. Koyama, S. Ozawa, and R.-K. Li Optimal Biomaterial for Creation of Autologous Cardiac Grafts Circulation, September 24, 2002; 106(12_suppl_1): I-176 - I-182. [Abstract] [Full Text] [PDF]

	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020