|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 November 2001, Vol. 98, No. 9, pp. 2673-2680
HEMATOPOIESIS
Forced expression of the Ikaros 6 isoform in human placental
blood CD34+ cells impairs their ability to differentiate
toward the B-lymphoid lineage
Cécile Tonnelle,
Florence Bardin,
Christine Maroc,
Anne-Marie Imbert,
Fanny Campa,
Ali Dalloul,
Christian Schmitt, and
Christian Chabannon
From the Institut Paoli-Calmettes, Centre
Régional de Lutte Contre le Cancer Provence-Alpes-Côte
d'Azur, Marseille, France; and Laboratoire d'Immunologie
Cellulaire, UMR7627 Centre National de la Recherche Scientifique,
Hôpital Pitié-Salpêtrière, Paris, France.
Studies in mice suggest that the Ikaros (Ik)
gene encodes several isoforms and is a critical regulator of
hematolymphoid differentiation. Little is known on the role of Ikaros
in human stem cell differentiation. Herein, the biological consequences
of the forced expression of Ikaros 6 (Ik6) in human placental blood
CD34+ progenitors are evaluated. Ik6 is one of the
isoforms produced from the Ikaros premessenger RNA by alternative
splicing and is thought to behave as a dominant negative isoform of the
gene product because it lacks the DNA binding domain present in
transcriptionally active isoforms. The results demonstrate that human
cord blood CD34+ cells that express high levels of Ik6 as a
result of retrovirally mediated gene transfer have a reduced capacity
to produce lymphoid B cells in 2 independent assays: (1) in vitro
reinitiation of human hematopoiesis during coculture with the MS-5
murine stromal cell line and (2) xenotransplantation in
nonobese diabetic-severe combined immunodeficient mice.
These results suggest that Ikaros plays an important role in stem cell
commitment in humans and that the balance between the different
isoforms is a key element of this regulatory system; they support the
hypothesis that posttranscriptional events can participate in the
control of human hematopoietic differentiation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
F. Ahmed, N. Arseni, H. Glimm, W. Hiddemann, C. Buske, and M. Feuring-Buske
Constitutive Expression of the ATP-Binding Cassette Transporter ABCG2 Enhances the Growth Potential of Early Human Hematopoietic Progenitors
Stem Cells,
March 1, 2008;
26(3):
810 - 818.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Dijon, F. Bardin, A. Murati, M. Batoz, C. Chabannon, and C. Tonnelle
The role of Ikaros in human erythroid differentiation
Blood,
February 1, 2008;
111(3):
1138 - 1146.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A.-M. Imbert, G. Belaaloui, F. Bardin, C. Tonnelle, M. Lopez, and C. Chabannon
CD99 expressed on human mobilized peripheral blood CD34+ cells is involved in transendothelial migration
Blood,
October 15, 2006;
108(8):
2578 - 2586.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Chadwick, F. Shojaei, L. Gallacher, and M. Bhatia
Smad7 alters cell fate decisions of human hematopoietic repopulating cells
Blood,
March 1, 2005;
105(5):
1905 - 1915.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-H. Wang, Z. Zhang, P. D. Burrows, H. Kubagawa, S. L. Bridges Jr, H. W. Findley, and M. D. Cooper
V(D)J recombinatorial repertoire diversification during intraclonal pro-B to B-cell differentiation
Blood,
February 1, 2003;
101(3):
1030 - 1037.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
