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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wagner, K. Articles by Eder, M. Search for Related Content PubMed PubMed Citation Articles by Wagner, K. Articles by Eder, M. Related Collections Signal Transduction Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 November 2001, Vol. 98, No. 9, pp. 2689-2696 HEMATOPOIESIS Inhibition of granulocyte-macrophage colony-stimulating factor receptor function by a splice variant of the common -receptor subunit Katharina Wagner, Sabine Kafert-Kasting, Gerhard Heil, Arnold Ganser, and Matthias Eder From the Department of Hematology and Oncology, Hannover Medical School, and the Center for Cell Therapy/Cytonet, Hannover, Germany. The receptors for human granuloctye-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and IL-5 are composed of a ligand-specific -chain (eg, -GM-CSF receptor [-GMR]) and a common -subunit (-GMR). Ligand binding is believed to induce assembly or conformational changes in preformed complexes containing more than one - and -subunit in the activated receptor complex. To analyze the function of a splice variant of -GMR with a truncation in the intracellular domain (-GMRIT), BaF-3 cells expressing human -GMR plus -GMR were transfected with -GMRIT. In these cells, coexpression of -GMRIT inhibits GM-CSF-mediated survival and proliferation in a GM-CSF concentration-dependent manner. To analyze the effect of cytoplasmic assembly of truncated and full-length intracellular -GMR sequences, -GMR and -GMRIT were coexpressed with different chimeric /-GMR constructs. Whereas both -GMR and -GMRIT generate high-affinity GMR complexes in the presence of /-GMR, -GMRIT inhibits while -GMR supports proliferation and cell survival mediated by /-GMR. Correspondingly, -GMR, but not -GMRIT, generates functional GMR complexes when coexpressed with a defective /-GMR construct. These data indicate that -GMRIT can inhibit survival and mitogenic signaling of the wild-type GMR and demonstrate that recruitment of alternatively spliced receptor subunits may regulate the function of heteromeric cytokine receptors. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. J Brennan, J. A Sharp, E. Khalil, M. R Digby, S. L Mailer, C. M Lefevre, and K. R Nicholas A population of mammary epithelial cells do not require hormones or growth factors to survive J. Endocrinol., March 1, 2008; 196(3): 483 - 496. [Abstract] [Full Text] [PDF] S. Wu, R. Gessner, T. Taube, A. von Stackelberg, G. Henze, and K. Seeger Expression of Interleukin-10 Splicing Variants Is a Positive Prognostic Feature in Relapsed Childhood Acute Lymphoblastic Leukemia J. Clin. Oncol., May 1, 2005; 23(13): 3038 - 3042. [Abstract] [Full Text] [PDF] J. Coers, C. Ranft, and R. C. Skoda A Truncated Isoform of c-Mpl with an Essential C-terminal Peptide Targets the Full-length Receptor for Degradation J. Biol. Chem., August 27, 2004; 279(35): 36397 - 36404. [Abstract] [Full Text] [PDF] C. A. Evans, S. Ariffin, A. Pierce, and A. D. Whetton Identification of primary structural features that define the differential actions of IL-3 and GM-CSF receptors Blood, October 16, 2002; 100(9): 3164 - 3174. [Abstract] [Full Text] [PDF]

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