|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 January 2002, Vol. 99, No. 1, pp. 159-167
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Shear-dependent tether formation during platelet translocation on
von Willebrand factor
Sacha M. Dopheide,
Mhairi
J. Maxwell, and
Shaun P. Jackson
From the Australian Centre for Blood Diseases,
Department of Medicine, Monash Medical School, Australia.
The adhesion and aggregation of platelets at sites of vascular
injury is dependent on the initial binding of the GP Ib/V/IX receptor
complex to immobilized von Willebrand factor (VWF). Under flow
conditions, this interaction supports platelet translocation that is
characteristically stop-start in nature. High resolution imaging of
platelets during surface translocation on immobilized VWF revealed that
thin membrane tethers (length: 0.91 µm-47.90 µm) were pulled from
the surface of these cells. Membrane tethers were dynamic structures
that extended from small, localized adhesion contacts under the
influence of flow. Perfusion of platelets in the presence of blocking
antibodies against integrin  IIb 3, or over
isolated A1 domains, demonstrated that the VWF-GP Ib interaction was
sufficient to induce membrane tether formation. The rate and extent of
tether elongation was shear-dependent (shear range: 150 s 1-10 000 s1), with mean tether length
ranging from 3.23 µm to 16.55 µm, tether frequency from 2.67% to
97.33%, and tether growth rate from 0.04 µm/sec to 8.39 µm/sec.
Tether formation and retraction did not require platelet activation;
however, the growth rate, lifetime, and dimensions were
significantly affected by the actin polymerization inhibitor,
cytochalasin D, and by chelating intracellular calcium. Single-cell
analysis revealed that formation of membrane tethers regulates the
stop-start phases of platelet translocation on VWF, suggesting a
potentially important role for this phenomenon in regulating the
dynamics of the platelet-VWF interaction under flow.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. M. Auger and S. P. Watson
Dynamic Tyrosine Kinase-Regulated Signaling and Actin Polymerisation Mediate Aggregate Stability Under Shear
Arterioscler. Thromb. Vasc. Biol.,
August 1, 2008;
28(8):
1499 - 1504.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Jackson
The growing complexity of platelet aggregation
Blood,
June 15, 2007;
109(12):
5087 - 5095.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. J. Clemetson
How complex is the platelet GP Ib complex?
Blood,
January 15, 2007;
109(2):
393 - 394.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Maxwell, E. Westein, W. S. Nesbitt, S. Giuliano, S. M. Dopheide, and S. P. Jackson
Identification of a 2-stage platelet aggregation process mediating shear-dependent thrombus formation
Blood,
January 15, 2007;
109(2):
566 - 576.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Nurden, N. Debili, W. Vainchenker, R. Bobe, R. Bredoux, E. Corvazier, R. Combrie, E. Fressinaud, D. Meyer, A. T. Nurden, et al.
Impaired megakaryocytopoiesis in type 2B von Willebrand disease with severe thrombocytopenia
Blood,
October 15, 2006;
108(8):
2587 - 2595.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. M. Ruggeri, J. N. Orje, R. Habermann, A. B. Federici, and A. J. Reininger
Activation-independent platelet adhesion and aggregation under elevated shear stress
Blood,
September 15, 2006;
108(6):
1903 - 1910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Jackson, P. Mangin, and Y. Yuan
"Pulling strings": shear, platelets, and microparticles
Blood,
May 1, 2006;
107(9):
3418 - 3419.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Reininger, H. F. G. Heijnen, H. Schumann, H. M. Specht, W. Schramm, and Z. M. Ruggeri
Mechanism of platelet adhesion to von Willebrand factor and microparticle formation under high shear stress
Blood,
May 1, 2006;
107(9):
3537 - 3545.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Maxwell, S. M. Dopheide, S. J. Turner, and S. P. Jackson
Shear Induces a Unique Series of Morphological Changes in Translocating Platelets: Effects of Morphology on Translocation Dynamics
Arterioscler. Thromb. Vasc. Biol.,
March 1, 2006;
26(3):
663 - 669.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Ramachandran, M. Williams, T. Yago, D. W. Schmidtke, and R. P. McEver
Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin
PNAS,
September 14, 2004;
101(37):
13519 - 13524.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Dyson, A. D. Munday, A. M. Kong, R. D. Huysmans, M. Matzaris, M. J. Layton, H. H. Nandurkar, M. C. Berndt, and C. A. Mitchell
SHIP-2 forms a tetrameric complex with filamin, actin, and GPIb-IX-V: localization of SHIP-2 to the activated platelet actin cytoskeleton
Blood,
August 1, 2003;
102(3):
940 - 948.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Navdaev and K. J. Clemetson
Glycoprotein Ib Cross-linking/Ligation on Echicetin-coated Surfaces or Echicetin-IgMkappa in Stirred Suspension Activates Platelets by Cytoskeleton Modulated Calcium Release
J. Biol. Chem.,
November 22, 2002;
277(48):
45928 - 45934.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Schoenwaelder, S. C. Hughan, K. Boniface, S. Fernando, M. Holdsworth, P. E. Thompson, H. H. Salem, and S. P. Jackson
RhoA Sustains Integrin alpha IIbbeta 3 Adhesion Contacts under High Shear
J. Biol. Chem.,
April 19, 2002;
277(17):
14738 - 14746.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
