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Blood, 1 January 2002, Vol. 99, No. 1, pp. 364-371
TRANSPLANTATION
A comparison of murine T-cell-depleted adult bone marrow and
full-term fetal blood cells in hematopoietic engraftment and immune
reconstitution
Benny J. Chen,
Xiuyu Cui,
Gregory D. Sempowski,
Maria E. Gooding,
Congxiao Liu,
Barton F. Haynes, and
Nelson J. Chao
From the Department of Medicine and Immunology, Human
Vaccine Institute, Duke University Medical Center, Durham, NC.
Umbilical cord blood has been increasingly used as a source of
hematopoietic stem cells. A major area of concern for the use of cord
blood transplantation is the delay in myeloid and lymphoid recovery. To
directly compare myeloid and lymphoid recovery using an animal model of
bone marrow and cord blood as sources of stem cells, hematopoietic
engraftment and immune recovery were studied following infusion
of T-cell-depleted adult bone marrow or full-term fetal blood cells,
as a model of cord blood in a murine allogeneic transplantation model
(C57BL/6 [H-2b]  BALB/c [H-2d]).
Allogeneic full-term fetal blood has poorer radioprotective capacity
but greater long-term engraftment potential on a cell-to-cell basis
compared with T-cell-depleted bone marrow. Allogeneic full-term fetal
blood recipients had decreased absolute numbers of T, B, and
dendritic cells compared with bone marrow recipients. Splenic T cells
in allogeneic full-term fetal blood recipients proliferated poorly,
were unable to generate cytotoxic effectors against third-party alloantigens in vitro, and failed to generate alloantigen-specific cytotoxic antibodies in vivo. In addition, reconstituting T cells in
fetal blood recipients had decreased mouse T-cell receptor single-joint excision circles compared with bone marrow recipients. At
a per-cell level, B cells from fetal blood recipients did not proliferate as well as those found in bone marrow recipients. These
results suggest that full-term fetal blood can engraft allogeneic hosts
across the major histocompatibility barrier with slower hematopoietic engraftment and impaired immune reconstitution.
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