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Blood, 1 January 2002, Vol. 99, No. 1, pp. 378-380
BRIEF REPORT
Identification of the hemoglobin scavenger receptor/CD163 as a
natural soluble protein in plasma
Holger Jon Møller,
Niels Anker Peterslund,
Jonas
Heilskov Graversen, and
Søren Kragh Moestrup
From the Department of Clinical Biochemistry and the
Department of Hematology, AAS Aarhus University Hospital, and
Department of Medical Biochemistry, University of Aarhus, Denmark.
The hemoglobin scavenger receptor (HbSR/CD163) is an
interleukin-6- and glucocorticoid-regulated
macrophage/monocyte receptor for uptake of
haptoglobin-hemoglobin complexes. Moreover, there are strong
indications that HbSR serves an anti-inflammatory function. Immunoprecipitation and immunoblotting enabled identification of a
soluble plasma form of HbSR (sHbSR) having an electrophoretic mobility
equal to that of recombinant HbSR consisting of the extracellular domain (scavenger receptor cysteine-rich 1-9). A sandwich
enzyme-linked immunosorbent assay was established and used to measure
the sHbSR level in 130 healthy subjects (median, 1.87 mg/L; range,
0.73-4.69 mg/L). To evaluate the sHbSR levels in conditions with
increased leukocyte stimulation and proliferation, 140 patients
admitted to a hematological department were screened. Several patients, with a broad spectrum of diagnoses, had a level of sHbSR above the
range of healthy persons. Patients with myelomonocytic leukemias and
pneumonia/sepsis exhibited the highest levels (up to 67.3 mg/L). In
conclusion, sHbSR is an abundant plasma protein potentially valuable in
monitoring patients with infections and myelomonocytic leukemia.
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