|
|
Prepublished online as a Blood First Edition Paper on April 30, 2002; DOI 10.1182/blood-2001-12-0191.
 Previous Article | Table of Contents | Next Article 
Blood, 1 June 2002, Vol. 99, No. 11, pp. 4021-4029
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Host defense role of platelets: engulfment of HIV and
Staphylococcus aureus occurs in a specific subcellular
compartment and is enhanced by platelet activation
Tayebeh Youssefian,
Arnaud Drouin,
Jean-Marc Massé,
Josette Guichard, and
Elisabeth
M. Cramer
From INSERM U 474, Institut Cochin, Paris, France, and
Faculté de Médecine Paris-Ile de France-Ouest, France.
Platelets can bind and phagocytose infectious microorganisms and so
enable their transport for a prolonged time. To investigate the
subcellular events of these interactions, platelets were incubated either with Staphylococcus aureus or with HIV and analyzed
by electron microscopy (EM) and immuno-EM. HIV and bacteria
internalization occurred exclusively within platelets showing
morphological evidence of activation. Platelet activation enhanced the
degree of bacterial internalization. Immunolabeling revealed that the
engulfing vacuoles and the open canalicular system (OCS) were composed
of distinct antigens. The engulfing vacuoles eventually became the site
of prominent  -granule release. In platelets incubated with HIV, characteristic endocytic vacuoles were identified close to the plasma
membrane, tightly surrounding 1 or 2 HIV particles. Virus particles
were also located within the OCS. Immunogold labeling for the viral
core protein p24 confirmed the presence of HIV within platelets.
Finally, examination of platelets from a patient with acquired
immunodeficiency syndrome and high viremia suggested that HIV
endocytosis may also occur in vivo.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
P. von Hundelshausen and C. Weber
Platelets as Immune Cells: Bridging Inflammation and Cardiovascular Disease
Circ. Res.,
January 5, 2007;
100(1):
27 - 40.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Chaipan, E. J. Soilleux, P. Simpson, H. Hofmann, T. Gramberg, A. Marzi, M. Geier, E. A. Stewart, J. Eisemann, A. Steinkasserer, et al.
DC-SIGN and CLEC-2 Mediate Human Immunodeficiency Virus Type 1 Capture by Platelets.
J. Virol.,
September 1, 2006;
80(18):
8951 - 8960.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-C. Rouyez, M. Lestingi, M. Charon, S. Fichelson, A. Buzyn, and I. Dusanter-Fourt
IFN Regulatory Factor-2 Cooperates with STAT1 to Regulate Transporter Associated with Antigen Processing-1 Promoter Activity
J. Immunol.,
April 1, 2005;
174(7):
3948 - 3958.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. White
Platelets Are Covercytes, Not Phagocytes.
Blood (ASH Annual Meeting Abstracts),
November 16, 2004;
104(11):
3533 - 3533.
[Abstract]
|
|
|
|
|
|
|
P. Pawar, P. K. Shin, S. A. Mousa, J. M. Ross, and K. Konstantopoulos
Fluid Shear Regulates the Kinetics and Receptor Specificity of Staphylococcus aureus Binding to Activated Platelets
J. Immunol.,
July 15, 2004;
173(2):
1258 - 1265.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R.-C. Mercier, R. M. Dietz, J. L. Mazzola, A. S. Bayer, and M. R. Yeaman
Beneficial Influence of Platelets on Antibiotic Efficacy in an In Vitro Model of Staphylococcus aureus-Induced Endocarditis
Antimicrob. Agents Chemother.,
July 1, 2004;
48(7):
2551 - 2557.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
