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Blood, 15 January 2002, Vol. 99, No. 2, pp. 472-478
HEMATOPOIESIS
Multiple hematopoietic cell lineages develop in vivo from
transplanted Pax5-deficient pre-B I-cell clones
Christoph Schaniel,
Ludovica Bruno,
Fritz Melchers, and
Antonius G. Rolink
From the Basel Institute for Immunology, Switzerland.
Pax5-deficient pre-B I-cell clones,
transplanted into natural killer
(NK)-cell-deficient RAG2 /
IL-2R / hosts, populate the NK-cell
compartment with functional NK cells. NK-cell generation from
Pax5/ pre-B I cells is also observed in
NK-cell-proficient Balb/c RAG2/ hosts. In
the same Balb/c RAG2/ hosts,
Pax5/ pre-B I-cell clones not only
populate the pre-B I-cell compartment and fill the deficient
T-cell-lineage compartment in the thymus and the periphery of all
hosts, as shown before, they also generate CD8 and
CD8+ dendritic cells (DCs), macrophages, and
granulocytes in vivo in approximately half the hosts. In some
recipients, practically all the mature myeloid cells are of
Pax5/ origin, indicating the effectiveness
by which Pax5/ pre-B I cells can compete
with endogenous myeloid precursors. In a smaller percentage of hosts,
the generation of Pax5/ pre-B
I-cell-derived erythrocytes is observed 4 to 6 months after transplantation. The results indicate that Pax5/
pre-B I cells can develop in vivo in hosts that have undergone transplantation to erythroid, myeloid, and lymphoid cell
lineages. Hence, the Pax5/ mutation
introduces an unusual instability of differentiation in pre-B I cells
so that they appear to dedifferentiate as far back as the pluripotent
hematopoietic stem cell.
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