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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jetmore, A. Articles by Srour, E. F. Search for Related Content PubMed PubMed Citation Articles by Jetmore, A. Articles by Srour, E. F. Related Collections Hematopoiesis and Stem Cells Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 March 2002, Vol. 99, No. 5, pp. 1585-1593 HEMATOPOIESIS Homing efficiency, cell cycle kinetics, and survival of quiescent and cycling human CD34+ cells transplanted into conditioned NOD/SCID recipients Anna Jetmore, P. Artur Plett, Xia Tong, Frances M. Wolber, Robert Breese, Rafat Abonour, Christie M. Orschell-Traycoff, and Edward F. Srour From the Departments of Medicine and Pediatrics, Herman B Wells Center for Pediatric Research and the Department of Microbiology/Immunology, Indiana University School of Medicine, Indianapolis. Differences in engraftment potential of hematopoietic stem cells (HSCs) in distinct phases of cell cycle may result from the inability of cycling cells to home to the bone marrow (BM) and may be influenced by the rate of entry of BM-homed HSCs into cell cycle. Alternatively, preferential apoptosis of cycling cells may contribute to their low engraftment potential. This study examined homing, cell cycle progression, and survival of human hematopoietic cells transplanted into nonobese diabetic severe combined immunodeficient (NOD/SCID) recipients. At 40 hours after transplantation (AT), only 1% of CD34+ cells, or their G0 (G0CD34+) or G1 (G1CD34+) subfractions, was detected in the BM of recipient mice, suggesting that homing of engrafting cells to the BM was not specific. BM of NOD/SCID mice receiving grafts containing approximately 50% CD34+ cells harbored similar numbers of CD34+ and CD34 cells, indicating that CD34+ cells did not preferentially traffic to the BM. Although more than 64% of human hematopoietic cells cycled in culture at 40 hours, more than 92% of cells recovered from NOD/SCID marrow were quiescent. Interestingly, more apoptotic human cells were detected at 40 hours AT in the BM of mice that received xenografts of expanded cells in S/G2+M than in recipients of G0/G1 cells (34.6% ± 5.9% and 17.1% ± 6.3%, respectively; P < .01). These results suggest that active proliferation inhibition in the BM of irradiated recipients maintains mitotic quiescence of transplanted HSCs early AT and may trigger apoptosis of cycling cells. These data also illustrate that trafficking of transplanted cells to the BM is not selective, but lodgment of BM-homed cells may be specific. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Hoggatt, P. Singh, J. Sampath, and L. M. Pelus Prostaglandin E2 enhances hematopoietic stem cell homing, survival, and proliferation Blood, May 28, 2009; 113(22): 5444 - 5455. [Abstract] [Full Text] [PDF] B. A. Poteat, B. R. Chitteti, K. E. Pollok, A. Suvannasankha, and E. F. Srour Differential Maintenance of Cell Cycle Status of Human Bone Marrow- and Umbilical Cord Blood-Derived CD34+ Cells after Homing to the Bone Marrow Microenvironment. 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