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Blood, 15 March 2002, Vol. 99, No. 6, pp. 2070-2076
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Shear-dependent morphology of von Willebrand factor
bound to immobilized collagen
Levente Novák,
Hans Deckmyn,
Sándor Damjanovich, and
Jolán Hársfalvi
From the University of Debrecen, Medical and Health
Science Center, Department of Clinical Biochemistry and Molecular
Pathology, and the Department of Biophysics and Cell Biology, Debrecen,
Hungary; and the Catholic University of Leuven, Campus Kortrijk,
Interdisciplinary Research Center, Kortrijk, Belgium.
We have developed an immunogold von Willebrand factor (VWF)
detection method that permits almost complete coverage of individual VWF molecules, and by this unequivocal localization and morphologic analysis of collagen-bound VWF by atomic force microscopy (AFM). Perfusion of gel filtration-purified VWF in parallel plate perfusion chambers over glass coverslips coated with calf skin collagen, followed
by AFM imaging in air, enabled us to assess possible morphologic
differences between VWF bound at low (0.07 N/m2 = 0.7
dynes/cm2) and high (4.55 N/m2 = 45.5
dynes/cm2) shear stresses. No significant differences in
VWF morphology were found, the molecules were oriented almost randomly,
and there were no clear signs of VWF "uncoiling" either at a high
or at a low shear regime. After perfusing 1 µg/mL VWF for 5 minutes, surface coverage at high shear was almost twice the one seen at low
shear, and some larger and more irregularly shaped VWF molecules could
be seen at high shear. This difference disappeared, however, at 15 minutes of perfusion and was probably caused by diffusion kinetics.
Moreover, the presence of 68 × 109/L washed fixed
platelets in the perfusate did not have any visible effect on VWF
morphology at high versus low shear stress. These findings suggest that
shear stress does not influence significantly the overall molecular
morphology of VWF during its binding to collagen-coated surface and are
consistent with a constitutively expressed affinity of collagen-bound
VWF for glycoprotein Ib.
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