SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Moutaftsi, M. Articles by Tabi, Z. Search for Related Content PubMed PubMed Citation Articles by Moutaftsi, M. Articles by Tabi, Z. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 April 2002, Vol. 99, No. 8, pp. 2913-2921 IMMUNOBIOLOGY Human cytomegalovirus inhibits maturation and impairs function of monocyte-derived dendritic cells Magdalena Moutaftsi, Anja M. Mehl, Leszek K. Borysiewicz, and Zsuzsanna Tabi From the Section of Infection and Immunity, University of Wales College of Medicine, Cardiff, United Kingdom, and the Imperial College School of Medicine, London, United Kingdom. Dendritic cells (DCs) play a pivotal role in the generation of virus-specific cytotoxic T-cell responses, but some viruses can render DCs inefficient in stimulating T cells. We studied whether infection of DCs with human cytomegalovirus (HCMV) results in a suppression of DC function which may assist HCMV in establishing persistence. The effect of HCMV infection on the phenotype and function of monocyte-derived DCs and on their ability to mature following infection with an endothelial cell-adapted clinical HCMV isolate were studied. HCMV infection induced no maturation of DCs; instead, it efficiently down-regulated the expression of surface major histocompatibility complex (MHC) class I, CD40, and CD80 molecules. Slight down-regulation of MHC class II and CD86 molecules was also observed. Lipopolysaccharide (LPS)-induced maturation of infected DCs was strongly inhibited, as indicated by lower levels of surface expression of MHC class I, class II, costimulatory, and CD83 molecules. The down-regulation or inhibition of these surface markers occurred only in HCMV antigen-positive DCs. DCs produced no interleukin 12 (IL-12) and only low levels of tumor necrosis factor alpha (TNF-) upon HCMV infection. Furthermore, cytokine production upon stimulation with LPS or CD40L was significantly impaired. Inhibition of cytokine production did not depend on viral gene expression as UV-irradiated HCMV resulted in the same effect. Proliferation and cytotoxicity of T cells specific to a recall antigen presented by DCs were also reduced when DCs were HCMV infected. This study shows that HCMV inhibits DC function, revealing a powerful viral strategy to delay or prevent the generation of virus-specific cytotoxic T cells. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: P. Jagannathan, C. M. Osborne, C. Royce, M. M. Manion, J. C. Tilton, L. Li, S. Fischer, C. W. Hallahan, J. A. Metcalf, M. McLaughlin, et al. Comparisons of CD8+ T Cells Specific for Human Immunodeficiency Virus, Hepatitis C Virus, and Cytomegalovirus Reveal Differences in Frequency, Immunodominance, Phenotype, and Interleukin-2 Responsiveness J. Virol., March 15, 2009; 83(6): 2728 - 2742. [Abstract] [Full Text] [PDF] S. Saghafian-Hedengren, Y. Sundstrom, E. Sohlberg, C. Nilsson, A. Linde, M. Troye-Blomberg, L. Berg, and E. Sverremark-Ekstrom Herpesvirus Seropositivity in Childhood Associates with Decreased Monocyte-Induced NK Cell IFN-{gamma} Production J. Immunol., February 15, 2009; 182(4): 2511 - 2517. [Abstract] [Full Text] [PDF] A. Saez-Borderias, N. Romo, G. Magri, M. Guma, A. Angulo, and M. Lopez-Botet IL-12-Dependent Inducible Expression of the CD94/NKG2A Inhibitory Receptor Regulates CD94/NKG2C+ NK Cell Function J. Immunol., January 15, 2009; 182(2): 829 - 836. [Abstract] [Full Text] [PDF] V. Bohm, C. O. Simon, J. Podlech, C. K. Seckert, D. Gendig, P. Deegen, D. Gillert-Marien, N. A. W. Lemmermann, R. Holtappels, and M. J. Reddehase The Immune Evasion Paradox: Immunoevasins of Murine Cytomegalovirus Enhance Priming of CD8 T Cells by Preventing Negative Feedback Regulation J. Virol., December 1, 2008; 82(23): 11637 - 11650. [Abstract] [Full Text] [PDF] S. Arimilli, J. B. Johnson, K. M. Clark, A. H. Graff, M. A. Alexander-Miller, S. B. Mizel, and G. D. Parks Engineered Expression of the TLR5 Ligand Flagellin Enhances Paramyxovirus Activation of Human Dendritic Cell Function J. Virol., November 15, 2008; 82(22): 10975 - 10985. [Abstract] [Full Text] [PDF] W. Dejnirattisai, T. Duangchinda, C.-L. S. Lin, S. Vasanawathana, M. Jones, M. Jacobs, P. Malasit, X.-n. Xu, G. Screaton, and J. Mongkolsapaya A Complex Interplay among Virus, Dendritic Cells, T Cells, and Cytokines in Dengue Virus Infections J. Immunol., November 1, 2008; 181(9): 5865 - 5874. [Abstract] [Full Text] [PDF] T. Kessler, M. Reich, G. Jahn, E. Tolosa, A. Beck, H. Kalbacher, H. Overkleeft, S. Schempp, and C. Driessen Human cytomegalovirus infection interferes with major histocompatibility complex type II maturation and endocytic proteases in dendritic cells at multiple levels J. Gen. Virol., October 1, 2008; 89(10): 2427 - 2436. [Abstract] [Full Text] [PDF] S. Munir, C. Le Nouen, C. Luongo, U. J. Buchholz, P. L. Collins, and A. Bukreyev Nonstructural Proteins 1 and 2 of Respiratory Syncytial Virus Suppress Maturation of Human Dendritic Cells J. Virol., September 1, 2008; 82(17): 8780 - 8796. [Abstract] [Full Text] [PDF] L. A. Smyth, N. Harker, W. Turnbull, H. El-Doueik, L. Klavinskis, D. Kioussis, G. Lombardi, and R. Lechler The Relative Efficiency of Acquisition of MHC:Peptide Complexes and Cross-Presentation Depends on Dendritic Cell Type J. Immunol., September 1, 2008; 181(5): 3212 - 3220. [Abstract] [Full Text] [PDF] M. J. Raftery, M. Hitzler, F. Winau, T. Giese, B. Plachter, S. H. E. Kaufmann, and G. Schonrich Inhibition of CD1 Antigen Presentation by Human Cytomegalovirus J. Virol., May 1, 2008; 82(9): 4308 - 4319. [Abstract] [Full Text] [PDF] C. Jenkins, W. Garcia, M. J. Godwin, J. V. Spencer, J. L. Stern, A. Abendroth, and B. Slobedman Immunomodulatory Properties of a Viral Homolog of Human Interleukin-10 Expressed by Human Cytomegalovirus during the Latent Phase of Infection J. Virol., April 1, 2008; 82(7): 3736 - 3750. [Abstract] [Full Text] [PDF] C. A. Benedict, A. Loewendorf, Z. Garcia, B. R. Blazar, and E. M. Janssen Dendritic Cell Programming by Cytomegalovirus Stunts Naive T Cell Responses via the PD-L1/PD-1 Pathway J. Immunol., April 1, 2008; 180(7): 4836 - 4847. [Abstract] [Full Text] [PDF] M. Mandron, H. Martin, B. Bonjean, J. Lule, E. Tartour, and C. Davrinche Dendritic cell-induced apoptosis of human cytomegalovirus-infected fibroblasts promotes cross-presentation of pp65 to CD8+ T cells J. Gen. Virol., January 1, 2008; 89(1): 78 - 86. [Abstract] [Full Text] [PDF] C. S. Wagner, L. Walther-Jallow, E. Buentke, H.-G. Ljunggren, A. Achour, and B. J. Chambers Human cytomegalovirus-derived protein UL18 alters the phenotype and function of monocyte-derived dendritic cells J. Leukoc. Biol., January 1, 2008; 83(1): 56 - 63. [Abstract] [Full Text] [PDF] S. Varani, M. Cederarv, S. Feld, C. Tammik, G. Frascaroli, M. P. Landini, and C. Soderberg-Naucler Human Cytomegalovirus Differentially Controls B Cell and T Cell Responses through Effects on Plasmacytoid Dendritic Cells J. Immunol., December 1, 2007; 179(11): 7767 - 7776. [Abstract] [Full Text] [PDF] R. Hochreiter, C. Ptaschinski, S. L. Kunkel, and R. Rochford Murine gammaherpesvirus-68 productively infects immature dendritic cells and blocks maturation J. Gen. Virol., July 1, 2007; 88(7): 1896 - 1905. [Abstract] [Full Text] [PDF] C. Molle, M. Nguyen, V. Flamand, J. Renneson, F. Trottein, D. De Wit, F. Willems, M. Goldman, and S. Goriely IL-27 Synthesis Induced by TLR Ligation Critically Depends on IFN Regulatory Factor 3 J. Immunol., June 15, 2007; 178(12): 7607 - 7615. [Abstract] [Full Text] [PDF] E. J. Wiertz, R. Devlin, H. L. Collins, and M. E. Ressing Herpesvirus Interference with Major Histocompatibility Complex Class II-Restricted T-Cell Activation J. Virol., May 1, 2007; 81(9): 4389 - 4396. [Full Text] [PDF] J. D. Mintern, E. J. Klemm, M. Wagner, M. E. Paquet, M. D. Napier, Y. M. Kim, U. H. Koszinowski, and H. L. Ploegh Viral Interference with B7-1 Costimulation: A New Role for Murine Cytomegalovirus Fc Receptor-1 J. Immunol., December 15, 2006; 177(12): 8422 - 8431. [Abstract] [Full Text] [PDF] A. N. Harman, J. Wilkinson, C. R. Bye, L. Bosnjak, J. L. Stern, M. Nicholle, J. Lai, and A. L. Cunningham HIV Induces Maturation of Monocyte-Derived Dendritic Cells and Langerhans Cells J. Immunol., November 15, 2006; 177(10): 7103 - 7113. [Abstract] [Full Text] [PDF] A. W. Lee, L. Hertel, R. K. Louie, T. Burster, V. Lacaille, A. Pashine, D. A. Abate, E. S. Mocarski, and E. D. Mellins Human Cytomegalovirus Alters Localization of MHC Class II and Dendrite Morphology in Mature Langerhans Cells J. Immunol., September 15, 2006; 177(6): 3960 - 3971. [Abstract] [Full Text] [PDF] K.-D. Park, L. Marti, J. Kurtzberg, and P. Szabolcs In vitro priming and expansion of cytomegalovirus-specific Th1 and Tc1 T cells from naive cord blood lymphocytes Blood, September 1, 2006; 108(5): 1770 - 1773. [Abstract] [Full Text] [PDF] G. Frascaroli, S. Varani, B. Moepps, C. Sinzger, M. P. Landini, and T. Mertens Human cytomegalovirus subverts the functions of monocytes, impairing chemokine-mediated migration and leukocyte recruitment. J. Virol., August 1, 2006; 80(15): 7578 - 7589. [Abstract] [Full Text] [PDF] G. Frascaroli, S. Varani, A. Mastroianni, S. Britton, D. Gibellini, G. Rossini, M. P. Landini, and C. Soderberg-Naucler Dendritic cell function in cytomegalovirus-infected patients with mononucleosis J. Leukoc. Biol., May 1, 2006; 79(5): 932 - 940. [Abstract] [Full Text] [PDF] S. Arimilli, M. A. Alexander-Miller, and G. D. Parks A Simian Virus 5 (SV5) P/V Mutant Is Less Cytopathic than Wild-Type SV5 in Human Dendritic Cells and Is a More Effective Activator of Dendritic Cell Maturation and Function. J. Virol., April 1, 2006; 80(7): 3416 - 3427. [Abstract] [Full Text] [PDF] E. O. Kvale, J. Dalgaard, F. Lund-Johansen, H. Rollag, L. Farkas, K. Midtvedt, F. L. Jahnsen, J. E. Brinchmann, and J. Olweus CD11c+ dendritic cells and plasmacytoid DCs are activated by human cytomegalovirus and retain efficient T cell-stimulatory capability upon infection Blood, March 1, 2006; 107(5): 2022 - 2029. [Abstract] [Full Text] [PDF] C. M. Bosio and S. W. Dow Francisella tularensis Induces Aberrant Activation of Pulmonary Dendritic Cells J. Immunol., November 15, 2005; 175(10): 6792 - 6801. [Abstract] [Full Text] [PDF] S. Coleman, A. Clayton, M. D. Mason, B. Jasani, M. Adams, and Z. Tabi Recovery of CD8+ T-Cell Function During Systemic Chemotherapy in Advanced Ovarian Cancer Cancer Res., August 1, 2005; 65(15): 7000 - 7006. [Abstract] [Full Text] [PDF] H. Barth, A. Ulsenheimer, G. R. Pape, H. M. Diepolder, M. Hoffmann, C. Neumann-Haefelin, R. Thimme, P. Henneke, R. Klein, G. Paranhos-Baccala, et al. Uptake and presentation of hepatitis C virus-like particles by human dendritic cells Blood, May 1, 2005; 105(9): 3605 - 3614. [Abstract] [Full Text] [PDF] A. P. Smith, C. Paolucci, G. Di Lullo, S. E. Burastero, F. Santoro, and P. Lusso Viral Replication-Independent Blockade of Dendritic Cell Maturation and Interleukin-12 Production by Human Herpesvirus 6 J. Virol., March 1, 2005; 79(5): 2807 - 2813. [Abstract] [Full Text] [PDF] G. Gerna, E. Percivalle, D. Lilleri, L. Lozza, C. Fornara, G. Hahn, F. Baldanti, and M. G. Revello Dendritic-cell infection by human cytomegalovirus is restricted to strains carrying functional UL131-128 genes and mediates efficient viral antigen presentation to CD8+ T cells J. Gen. Virol., February 1, 2005; 86(2): 275 - 284. [Abstract] [Full Text] [PDF] S. Varani, G. Frascaroli, M. Homman-Loudiyi, S. Feld, M. P. Landini, and C. Soderberg-Naucler Human cytomegalovirus inhibits the migration of immature dendritic cells by down-regulating cell-surface CCR1 and CCR5 J. Leukoc. Biol., February 1, 2005; 77(2): 219 - 228. [Abstract] [Full Text] [PDF] A. Loewendorf, C. Kruger, E. M. Borst, M. Wagner, U. Just, and M. Messerle Identification of a Mouse Cytomegalovirus Gene Selectively Targeting CD86 Expression on Antigen-Presenting Cells J. Virol., December 1, 2004; 78(23): 13062 - 13071. [Abstract] [Full Text] [PDF] A. Smed-Sorensen, K. Lore, L. Walther-Jallow, J. Andersson, and A.-L. Spetz HIV-1-infected dendritic cells up-regulate cell surface markers but fail to produce IL-12 p70 in response to CD40 ligand stimulation Blood, November 1, 2004; 104(9): 2810 - 2817. [Abstract] [Full Text] [PDF] M. J. Raftery, D. Wieland, S. Gronewald, A. A. Kraus, T. Giese, and G. Schonrich Shaping Phenotype, Function, and Survival of Dendritic Cells by Cytomegalovirus-Encoded IL-10 J. Immunol., September 1, 2004; 173(5): 3383 - 3391. [Abstract] [Full Text] [PDF] W. L. W. Chang, N. Baumgarth, D. Yu, and P. A. Barry Human Cytomegalovirus-Encoded Interleukin-10 Homolog Inhibits Maturation of Dendritic Cells and Alters Their Functionality J. Virol., August 15, 2004; 78(16): 8720 - 8731. [Abstract] [Full Text] [PDF] F. Goodrum, C. T. Jordan, S. S. Terhune, K. High, and T. Shenk Differential outcomes of human cytomegalovirus infection in primitive hematopoietic cell subpopulations Blood, August 1, 2004; 104(3): 687 - 695. [Abstract] [Full Text] [PDF] B. Senechal, A. M. Boruchov, J. L. Reagan, D. N. J. Hart, and J. W. Young Infection of mature monocyte-derived dendritic cells with human cytomegalovirus inhibits stimulation of T-cell proliferation via the release of soluble CD83 Blood, June 1, 2004; 103(11): 4207 - 4215. [Abstract] [Full Text] [PDF] A. Granelli-Piperno, A. Golebiowska, C. Trumpfheller, F. P. Siegal, and R. M. Steinman HIV-1-infected monocyte-derived dendritic cells do not undergo maturation but can elicit IL-10 production and T cell regulation PNAS, May 18, 2004; 101(20): 7669 - 7674. [Abstract] [Full Text] [PDF] M. Moutaftsi, P. Brennan, S. A. Spector, and Z. Tabi Impaired Lymphoid Chemokine-Mediated Migration due to a Block on the Chemokine Receptor Switch in Human Cytomegalovirus-Infected Dendritic Cells J. Virol., March 15, 2004; 78(6): 3046 - 3054. [Abstract] [Full Text] [PDF] W. Tu, S. Chen, M. Sharp, C. Dekker, A. M. Manganello, E. C. Tongson, H. T. Maecker, T. H. Holmes, Z. Wang, G. Kemble, et al. Persistent and Selective Deficiency of CD4+ T Cell Immunity to Cytomegalovirus in Immunocompetent Young Children J. Immunol., March 1, 2004; 172(5): 3260 - 3267. [Abstract] [Full Text] [PDF] P. Sarobe, J. J. Lasarte, A. Zabaleta, L. Arribillaga, A. Arina, I. Melero, F. Borras-Cuesta, and J. Prieto Hepatitis C Virus Structural Proteins Impair Dendritic Cell Maturation and Inhibit In Vivo Induction of Cellular Immune Responses J. Virol., October 15, 2003; 77(20): 10862 - 10871. [Abstract] [Full Text] [PDF] S. Gredmark and C. Soderberg-Naucler Human Cytomegalovirus Inhibits Differentiation of Monocytes into Dendritic Cells with the Consequence of Depressed Immunological Functions J. Virol., October 15, 2003; 77(20): 10943 - 10956. [Abstract] [Full Text] [PDF] X. Wang, M. Messerle, R. Sapinoro, K. Santos, P. K. Hocknell, X. Jin, and S. Dewhurst Murine Cytomegalovirus Abortively Infects Human Dendritic Cells, Leading to Expression and Presentation of Virally Vectored Genes J. Virol., July 1, 2003; 77(13): 7182 - 7192. [Abstract] [Full Text] [PDF] L. Hertel, V. G. Lacaille, H. Strobl, E. D. Mellins, and E. S. Mocarski Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus J. Virol., July 1, 2003; 77(13): 7563 - 7574. [Abstract] [Full Text] [PDF] L. E. Gamadia, E. B. M. Remmerswaal, J. F. Weel, F. Bemelman, R. A. W. van Lier, and I. J. M. Ten Berge Primary immune responses to human CMV: a critical role for IFN-gamma -producing CD4+ T cells in protection against CMV disease Blood, April 1, 2003; 101(7): 2686 - 2692. [Abstract] [Full Text] [PDF] V. J. Cavanaugh, Y. Deng, M. P. Birkenbach, J. S. Slater, and A. E. Campbell Vigorous Innate and Virus-Specific Cytotoxic T-Lymphocyte Responses to Murine Cytomegalovirus in the Submaxillary Salivary Gland J. Virol., February 1, 2003; 77(3): 1703 - 1717. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020