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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Moutaftsi, M. Articles by Tabi, Z. Search for Related Content PubMed PubMed Citation Articles by Moutaftsi, M. Articles by Tabi, Z. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 April 2002, Vol. 99, No. 8, pp. 2913-2921 IMMUNOBIOLOGY Human cytomegalovirus inhibits maturation and impairs function of monocyte-derived dendritic cells Magdalena Moutaftsi, Anja M. Mehl, Leszek K. Borysiewicz, and Zsuzsanna Tabi From the Section of Infection and Immunity, University of Wales College of Medicine, Cardiff, United Kingdom, and the Imperial College School of Medicine, London, United Kingdom. Dendritic cells (DCs) play a pivotal role in the generation of virus-specific cytotoxic T-cell responses, but some viruses can render DCs inefficient in stimulating T cells. We studied whether infection of DCs with human cytomegalovirus (HCMV) results in a suppression of DC function which may assist HCMV in establishing persistence. The effect of HCMV infection on the phenotype and function of monocyte-derived DCs and on their ability to mature following infection with an endothelial cell-adapted clinical HCMV isolate were studied. HCMV infection induced no maturation of DCs; instead, it efficiently down-regulated the expression of surface major histocompatibility complex (MHC) class I, CD40, and CD80 molecules. Slight down-regulation of MHC class II and CD86 molecules was also observed. Lipopolysaccharide (LPS)-induced maturation of infected DCs was strongly inhibited, as indicated by lower levels of surface expression of MHC class I, class II, costimulatory, and CD83 molecules. The down-regulation or inhibition of these surface markers occurred only in HCMV antigen-positive DCs. DCs produced no interleukin 12 (IL-12) and only low levels of tumor necrosis factor alpha (TNF-) upon HCMV infection. Furthermore, cytokine production upon stimulation with LPS or CD40L was significantly impaired. Inhibition of cytokine production did not depend on viral gene expression as UV-irradiated HCMV resulted in the same effect. Proliferation and cytotoxicity of T cells specific to a recall antigen presented by DCs were also reduced when DCs were HCMV infected. This study shows that HCMV inhibits DC function, revealing a powerful viral strategy to delay or prevent the generation of virus-specific cytotoxic T cells. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Mittal, S. Bulgheresi, C. Emami, and N. V. Prasadarao Enterobacter sakazakii Targets DC-SIGN to Induce Immunosuppressive Responses in Dendritic Cells by Modulating MAPKs J. Immunol., November 15, 2009; 183(10): 6588 - 6599. [Abstract] [Full Text] [PDF] S. M. Hughes, B. Amadi, M. Mwiya, H. Nkamba, A. Tomkins, and D. Goldblatt Dendritic Cell Anergy Results from Endotoxemia in Severe Malnutrition J. Immunol., August 15, 2009; 183(4): 2818 - 2826. [Abstract] [Full Text] [PDF] P. Jagannathan, C. M. Osborne, C. Royce, M. M. Manion, J. C. Tilton, L. Li, S. Fischer, C. W. Hallahan, J. A. Metcalf, M. McLaughlin, et al. 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