|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 April 2002, Vol. 99, No. 8, pp. 2922-2928
IMMUNOBIOLOGY
Level of galactosylation determines cryoglobulin activity of
murine IgG3 monoclonal rheumatoid factor
Aki Kuroki,
Yasuhiro Kuroda,
Shuichi Kikuchi,
Frédéric Lajaunias,
Thierry Fulpius,
Yves Pastore,
Liliane Fossati-Jimack,
Luc Reininger,
Toho Toda,
Munehiro Nakata,
Naoya Kojima,
Tsuguo Mizuochi, and
Shozo Izui
From the Department of Pathology, Faculty of Medicine,
University of Geneva, Switzerland; Institute of Glycotechnology and
Department of Applied Biochemistry, Tokai University, Hiratsuka,
Kanagawa, Japan; and Institut National de la Santé et de la
Recherche Médicale U 399, Marseille, France.
Autoantibodies of the cryoprecipitating IgG3 isotype have
been shown to play a significant role in the development of murine lupus-like autoimmune syndrome. At present, the structural basis of
IgG3 cryoprecipitation and its role in autoantibody pathogenicity remain to be defined. Using molecular variants of an IgG3 monoclonal rheumatoid factor, 6-19, derived from an autoimmune
MRL-Faslpr mouse, we have investigated the
implication of charged residues in the heavy-chain variable (VH)
region, potential CH3-linked oligosaccharides, and galactosylation of
CH2-linked oligosaccharides in its cryoglobulin activity. The
cryoglobulin activity of the IgG3 6-19 mutant bearing more negatively
charged residues at VH 6 and 23 was found to be reduced but still
highly significant, whereas that of the mutant lacking a potential CH3
glycosylation site remained unchanged. In marked contrast, IgG3 6-19 variants obtained from 6-19 heavy-chain transgenic mice displayed
barely detectable cryoglobulin activity associated with an increased level of galactosylation in the CH2 oligosaccharide side chains. Thus,
our data strongly suggest that the cryoglobulin activity of IgG3 6-19 autoantibody is critically determined by levels of galactosylation in
the CH2 oligosaccharide side chains, whereas VH residues play a
secondary role in 6-19 IgG3 cryoglobulin activity.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Baudino, Y. Shinohara, F. Nimmerjahn, J.-I. Furukawa, M. Nakata, E. Martinez-Soria, F. Petry, J. V. Ravetch, S.-I. Nishimura, and S. Izui
Crucial Role of Aspartic Acid at Position 265 in the CH2 Domain for Murine IgG2a and IgG2b Fc-Associated Effector Functions
J. Immunol.,
November 1, 2008;
181(9):
6664 - 6669.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Baudino, F. Nimmerjahn, S. Azeredo da Silveira, E. Martinez-Soria, T. Saito, M. Carroll, J. V. Ravetch, J. S. Verbeek, and S. Izui
Differential Contribution of Three Activating IgG Fc Receptors (Fc{gamma}RI, Fc{gamma}RIII, and Fc{gamma}RIV) to IgG2a- and IgG2b-Induced Autoimmune Hemolytic Anemia in Mice
J. Immunol.,
February 1, 2008;
180(3):
1948 - 1953.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Baudino, L. Fossati-Jimack, C. Chevalley, E. Martinez-Soria, M. J. Shulman, and S. Izui
IgM and IgA anti-erythrocyte autoantibodies induce anemia in a mouse model through multivalency-dependent hemagglutination but not through complement activation
Blood,
June 15, 2007;
109(12):
5355 - 5362.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Bobe, D. Bonardelle, K. Benihoud, P. Opolon, and M. K. Chelbi-Alix
Arsenic trioxide: a promising novel therapeutic agent for lymphoproliferative and autoimmune syndromes in MRL/lpr mice
Blood,
December 15, 2006;
108(13):
3967 - 3975.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kuroda, A. Kuroki, S. Kikuchi, T. Funase, M. Nakata, and S. Izui
A Critical Role for Sialylation in Cryoglobulin Activity of Murine IgG3 Monoclonal Antibodies
J. Immunol.,
July 15, 2005;
175(2):
1056 - 1061.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
