|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 May 2002, Vol. 99, No. 9, pp. 3310-3318
IMMUNOBIOLOGY
Interleukin-7-treated naive T cells can be productively infected
by T-cell-adapted and primary isolates of human immunodeficiency
virus 1
Carolyn M. Steffens,
Elizabeth Z. Managlia,
Alan Landay, and
Lena Al-Harthi
From the Department of Immunology/Microbiology at
Rush-Presbyterian-St Luke's Medical Center, Rush University,
Chicago, IL.
Although human immunodeficiency virus (HIV)
gag/pol DNA can be detected in naive T cells,
whether naive T cells can be productively infected by HIV is still
questionable. Given that interleukin-7 (IL-7) is a prospective
therapeutic immunomodulator for the treatment of HIV, we
evaluated the effect of IL-7 on promoting naive T-cell infection
of laboratory-adapted (IIIB), M-tropic, and primary isolates of HIV.
Initially, we determined that the 3 cell surface markers widely used to
identify naive T cells (CD45RA+CD45RO ,
CD45RA+CD62L+, and
CD45ROCD27+CD95low) are all
equivalent in T-cell receptor excision circle content, a marker for the
replicative history of a cell as well as for de novo T cells. We
therefore used CD45RA+CD45RO expression to
define naive T cells in this study. We demonstrate that although
untreated or IL-2-treated naive T cells are not productively infected
by HIV, IL-7 pretreatment mediated the productive infection of
laboratory-adapted, M-tropic, and primary isolates of HIV as determined
by p24 core antigen production. This up-regulation was between 8- and
58-fold, depending on the HIV isolate used. IL-7 pretreatment of naive
T cells also potently up-regulated surface expression of CXCR4 but not
CCR5 and mediated the expansion of naive T cells without the
acquisition of the primed CD45RO phenotype. Collectively, these data
indicate that IL-7 augments naive T-cell susceptibility to HIV and that
under the appropriate environmental milieu, naive T cells may be a
source of HIV productive infection. This information needs to be
considered in evaluating IL-7 as an immunomodulator for HIV-infected patients.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
V. Ostiguy, E.-L. Allard, M. Marquis, J. Leignadier, and N. Labrecque
IL-21 promotes T lymphocyte survival by activating the phosphatidylinositol-3 kinase signaling cascade
J. Leukoc. Biol.,
September 1, 2007;
82(3):
645 - 656.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Hryniewicz, D. A. Price, M. Moniuszko, A. Boasso, Y. Edghill-Spano, S. M. West, D. Venzon, M. Vaccari, W.-P. Tsai, E. Tryniszewska, et al.
Interleukin-15 but Not Interleukin-7 Abrogates Vaccine-Induced Decrease in Virus Level in Simian Immunodeficiency Virusmac251-Infected Macaques
J. Immunol.,
March 15, 2007;
178(6):
3492 - 3504.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Swainson, S. Kinet, C. Mongellaz, M. Sourisseau, T. Henriques, and N. Taylor
IL-7-induced proliferation of recent thymic emigrants requires activation of the PI3K pathway
Blood,
February 1, 2007;
109(3):
1034 - 1042.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Oswald-Richter, V. J. Torres, M. S. Sundrud, S. E. VanCompernolle, T. L. Cover, and D. Unutmaz
Helicobacter pylori VacA Toxin Inhibits Human Immunodeficiency Virus Infection of Primary Human T Cells
J. Virol.,
December 1, 2006;
80(23):
11767 - 11775.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Zaunders, S. Ip, M. L. Munier, D. E. Kaufmann, K. Suzuki, C. Brereton, S. C. Sasson, N. Seddiki, K. Koelsch, A. Landay, et al.
Infection of CD127+ (Interleukin-7 Receptor+) CD4+ Cells and Overexpression of CTLA-4 Are Linked to Loss of Antigen-Specific CD4 T Cells during Primary Human Immunodeficiency Virus Type 1 Infection.
J. Virol.,
October 1, 2006;
80(20):
10162 - 10172.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Zhang, J. Drenkow, C. S. R. Lankford, D. M. Frucht, R. L. Rabin, T. R. Gingeras, C. Venkateshan, F. Schwartzkopff, K. A. Clouse, and A. I. Dayton
HIV regulation of the IL-7R: a viral mechanism for enhancing HIV-1 replication in human macrophages in vitro
J. Leukoc. Biol.,
June 1, 2006;
79(6):
1328 - 1338.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Swainson, E. Verhoeyen, F.-L. Cosset, and N. Taylor
IL-7R{alpha} Gene Expression Is Inversely Correlated with Cell Cycle Progression in IL-7-Stimulated T Lymphocytes.
J. Immunol.,
June 1, 2006;
176(11):
6702 - 6708.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Gondois-Rey, A. Biancotto, M. A. Fernandez, L. Bettendroffer, J. Blazkova, K. Trejbalova, M. Pion, and I. Hirsch
R5 Variants of Human Immunodeficiency Virus Type 1 Preferentially Infect CD62L- CD4+ T Cells and Are Potentially Resistant to Nucleoside Reverse Transcriptase Inhibitors
J. Virol.,
January 15, 2006;
80(2):
854 - 865.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Carroll-Anzinger and L. Al-Harthi
Gamma Interferon Primes Productive Human Immunodeficiency Virus Infection in Astrocytes
J. Virol.,
January 1, 2006;
80(1):
541 - 544.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Audige, E. Schlaepfer, H. Joller, and R. F. Speck
Uncoupled Anti-HIV and Immune-Enhancing Effects when Combining IFN-{alpha} and IL-7
J. Immunol.,
September 15, 2005;
175(6):
3724 - 3736.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-D. Lelievre, F. Petit, D. Arnoult, J.-C. Ameisen, and J. Estaquier
Interleukin 7 Increases Human Immunodeficiency Virus Type 1 LAI-Mediated Fas-Induced T-Cell Death
J. Virol.,
March 1, 2005;
79(5):
3195 - 3199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Bahbouhi, A. Landay, and L. Al-Harthi
Dynamics of cytokine expression in HIV productively infected primary CD4+ T cells
Blood,
June 15, 2004;
103(12):
4581 - 4587.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-T. Nugeyre, V. Monceaux, S. Beq, M.-C. Cumont, R. H. T. Fang, L. Chene, M. Morre, F. Barre-Sinoussi, B. Hurtrel, and N. Israel
IL-7 Stimulates T Cell Renewal Without Increasing Viral Replication in Simian Immunodeficiency Virus-Infected Macaques
J. Immunol.,
October 15, 2003;
171(8):
4447 - 4453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. de la Rosa and M. Leal
Thymic involvement in recovery of immunity among HIV-infected adults on highly active antiretroviral therapy
J. Antimicrob. Chemother.,
August 1, 2003;
52(2):
155 - 158.
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Napolitano, C. A. Stoddart, M. B. Hanley, E. Wieder, and J. M. McCune
Effects of IL-7 on Early Human Thymocyte Progenitor Cells In Vitro and in SCID-hu Thy/Liv Mice
J. Immunol.,
July 15, 2003;
171(2):
645 - 654.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Cavalieri, S. Cazzaniga, M. Geuna, Z. Magnani, C. Bordignon, L. Naldini, and C. Bonini
Human T lymphocytes transduced by lentiviral vectors in the absence of TCR activation maintain an intact immune competence
Blood,
July 15, 2003;
102(2):
497 - 505.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Jaleco, L. Swainson, V. Dardalhon, M. Burjanadze, S. Kinet, and N. Taylor
Homeostasis of Naive and Memory CD4+ T Cells: IL-2 and IL-7 Differentially Regulate the Balance Between Proliferation and Fas-Mediated Apoptosis
J. Immunol.,
July 1, 2003;
171(1):
61 - 68.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Llano, J. Barretina, A. Gutierrez, B. Clotet, and J. A. Este
Interleukin-7-Dependent Production of RANTES That Correlates with Human Immunodeficiency Virus Disease Progression
J. Virol.,
April 1, 2003;
77(7):
4389 - 4395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Verhoeyen, V. Dardalhon, O. Ducrey-Rundquist, D. Trono, N. Taylor, and F.-L. Cosset
IL-7 surface-engineered lentiviral vectors promote survival and efficient gene transfer in resting primary T lymphocytes
Blood,
March 15, 2003;
101(6):
2167 - 2174.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Jaleco, S. Kinet, J. Hassan, V. Dardalhon, L. Swainson, D. Reen, N. Taylor, and L. Al-Harthi
IL-7 and CD4+ T-cell proliferation
Blood,
December 15, 2002;
100(13):
4676 - 4677.
[Full Text]
[PDF]
|
|
|
|
|
