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Blood, 1 December 2006, Vol. 108, No. 12, pp. 3662-3667. Prepublished online as a Blood First Edition Paper on August 15, 2006; DOI 10.1182/blood-2006-06-030577. This Article Full Text (PDF) All Versions of this Article: blood-2006-06-030577v1 108/12/3662    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shepherd, R. M Articles by Link, D. C Search for Related Content PubMed PubMed Citation Articles by Shepherd, R. M Articles by Link, D. C Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 21, 2006 Accepted July 18, 2006 Angiogenic cells can be rapidly mobilized and efficiently harvested from the blood following treatment with AMD3100 Rebecca M Shepherd, Benjamin J Capoccia, Steven M Devine, John DiPersio, Kathryn M Trinkaus, David Ingram, and Daniel C Link* Washington University School of Medicine, Saint Louis, Missouri, USA Ohio State University School of Medicine and Public Health, Ohio, USA Herman B. Wells Center for Pediatric Research, Indiana Univesity School of Medicine, Indiana, USA * Corresponding author; email: dlink{at}im.wustl.edu. Circulating endothelial progenitor cells (EPC) are thought to contribute to angiogenesis following vascular injury, stimulating interest in their ability to mediate therapeutic angiogenesis. However, the number of EPC in the blood is low, limiting endogenous repair, and a method to rapidly mobilize EPC has not been reported. In this study, healthy donors were mobilized sequentially with the CXCR4 antagonist, AMD3100, and G-CSF. The number of EPC and circulating angiogenic cells (CAC) in blood and pheresis product was determined and the angiogenic capacity of each cell population assessed. Compared to baseline, treatment with AMD3100 or G-CSF increased the number of blood CAC 10.0± 4.4 and 8.8± 3.7-fold, respectively. The number of EPC in the blood increased 10.2± 3.3 and 21.8± 5.4-fold, respectively. On a per cell basis, CAC harvested from G-CSF mobilized blood displayed increased in vivo angiogenic potential compared with AMD3100 mobilized CAC. Mobilized EPC displayed a greater proliferative capacity than EPC isolated from baseline blood. Both CAC and EPC were efficiently harvested by leukapheresis. Cryopreserved CAC but not EPC retained functional activity after thawing. These data show that AMD3100 is a potent and rapid mobilizer of angiogenic cells and demonstrates the feasibility of obtaining and storing large numbers of angiogenic cells by leukapheresis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: "Quick" fix to add to the mix? Mervin C. Yoder Blood 2006 108: 3625-3626. [Full Text] [PDF] This article has been cited by other articles: A. Reinisch, N. A. Hofmann, A. C. Obenauf, K. Kashofer, E. Rohde, K. Schallmoser, K. Flicker, G. Lanzer, W. Linkesch, M. R. Speicher, et al. Humanized large-scale expanded endothelial colony-forming cells function in vitro and in vivo Blood, June 25, 2009; 113(26): 6716 - 6725. [Abstract] [Full Text] [PDF] Y. J. Cao, C. W. Flexner, S. Dunaway, J.-G. Park, K. Klingman, I. Wiggins, J. Conley, C. Radebaugh, A. D. Kashuba, R. MacFarland, et al. Effect of Low-Dose Ritonavir on the Pharmacokinetics of the CXCR4 Antagonist AMD070 in Healthy Volunteers Antimicrob. Agents Chemother., May 1, 2008; 52(5): 1630 - 1634. [Abstract] [Full Text] [PDF] K. Asosingh, M. A. Aldred, A. Vasanji, J. Drazba, J. Sharp, C. Farver, S. A.A. Comhair, W. Xu, L. Licina, L. Huang, et al. Circulating Angiogenic Precursors in Idiopathic Pulmonary Arterial Hypertension Am. J. Pathol., March 1, 2008; 172(3): 615 - 627. [Abstract] [Full Text] [PDF] S. Nogueras, A. Merino, R. Ojeda, J. Carracedo, M. Rodriguez, A. Martin-Malo, R. Ramirez, and P. Aljama Coupling of endothelial injury and repair: an analysis using an in vivo experimental model Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H708 - H713. [Abstract] [Full Text] [PDF] A. Zernecke, I. Bot, Y. Djalali-Talab, E. Shagdarsuren, K. Bidzhekov, S. Meiler, R. Krohn, A. Schober, M. Sperandio, O. Soehnlein, et al. Protective Role of CXC Receptor 4/CXC Ligand 12 Unveils the Importance of Neutrophils in Atherosclerosis Circ. Res., February 1, 2008; 102(2): 209 - 217. [Abstract] [Full Text] [PDF] C. Xu, J. Sui, H. Tao, Q. Zhu, and W. A. Marasco Human Anti-CXCR4 Antibodies Undergo VH Replacement, Exhibit Functional V-Region Sulfation, and Define CXCR4 Antigenic Heterogeneity J. Immunol., August 15, 2007; 179(4): 2408 - 2418. [Abstract] [Full Text] [PDF]

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