SEARCH:   Advanced

Blood, 15 October 2008, Vol. 112, No. 8, pp. 3425-3433. Prepublished online as a Blood First Edition Paper on June 10, 2008; DOI 10.1182/blood-2008-02-137372. This Article Full Text (PDF) Supplemental Table and Figure All Versions of this Article: blood-2008-02-137372v1 112/8/3425    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rimsza, L. M. Articles by Gascoyne, R. D Search for Related Content PubMed PubMed Citation Articles by Rimsza, L. M. Articles by Gascoyne, R. D Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 5, 2008 Accepted May 17, 2008 Gene expression predicts overall survival in paraffin embedded tissues of diffuse large B cell lymphoma treated with R-CHOP Lisa M. Rimsza*, Michael L LeBlanc, Joseph M Unger, Thomas P Miller, Thomas M Grogan, Daniel O Persky, Ralph R Martel, Constantine M Sabalos, Bruce Seligmann, Rita M Braziel, Elias Campo, Andreas Rosenwald, Joseph M Connors, Laurie H Sehn, Nathalie Johnson, and Randy D Gascoyne Pathology Department, University of Arizona, Tucson, AZ, United States Public Health Sciences, Fred Hutchison Cancer Research Center, Seattle, WA, United States Arizona Cancer Center, University of Arizona, Tucson, AZ, United States High Throughput Genomics, Inc., Tucson, AZ, United States Pathology Department, OHSU, Portland, OR, United States Pathology Department, University of Barcelona, Barcelona, Spain Pathology Department, Universtiy of Wurzberg, Wurzburg, Germany Department of Medical Oncology, British Columbia Cancer Agency, Vancouver, British Columbia, Canada Pathology Department, British Columbia Cancer Agency, Vancouver, British Columbia, Canada * Corresponding author; email: lrimsza{at}email.arizona.edu. Gene expression profiling (GEP) on frozen tissues has identified genes predicting outcome in patients with diffuse large B cell lymphoma (DLBCL). Confirmation of results in current patients is limited by availability of frozen samples and addition of monoclonal antibodies to treatment regimens. We used a quantitative nuclease protection assay (qNPA) to analyze formalin-fixed, paraffin embedded tissue (FFPET) blocks for 36 previously identified genes (N=209, 93 chemotherapy; 116 rituximab+chemotherapy). By qNPA, 208 cases were successfully analyzed (99.5%). Additionally, 15/36 and 11/36 genes, representing each functional group previously identified by GEP, were associated with survival (p < 0.05) in the 2 treatment groups respectively. Additionally, 30/36 hazard ratios of death trended in the same direction compared to the original studies. Multivariate and variable cut point analysis identified low levels of HLA-DR (<20%) and high levels of MYC (>80%) as independent indicators of survival, together distinguishing cases with the worst prognosis. Our results solve a clinical research problem by demonstrating that: prognostic genes can be meaningfully quantified using the qNPA technology on FFPET blocks; previous GEP findings in DLBCL are relevant with current treatments; and 2 genes, representing immune escape and proliferation, are the common features of the most aggressive DLBCL. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Blocks to paraffin get the CHOP, +R Richard Byers Blood 2008 112: 3006-3007. [Full Text] [PDF] This article has been cited by other articles: L. M. Rimsza, W. C. Chan, R. D. Gascoyne, E. Campo, E. S. Jaffe, L. M. Staudt, J. Delabie, A. Rosenwald, and S. P. Murphy CIITA or RFX coding region loss of function mutations occur rarely in diffuse large B-cell lymphoma cases and cell lines with low levels of major histocompatibility complex class II expression Haematologica, April 1, 2009; 94(4): 596 - 598. [Full Text] [PDF]

	Copyright © 2008 by American Society of Hematology         Online ISSN: 1528-0020