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Blood First Edition Paper, prepublished online November 6, 2009; DOI 10.1182/blood-2009-05-219923. This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Han, J. Articles by Koh, G. Y. PubMed PubMed Citation Articles by Han, J. Articles by Koh, G. Y. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 6, 2009; accepted September 25, 2009. Adipose tissue is an extramedullary reservoir for functional hematopoietic stem and progenitor cells Jinah Han1, Young Jun Koh1, Hye Rin Moon2, Hyun Gee Ryoo2, Chung-Hyun Cho3, Injune Kim4 and Gou Young Koh1,3 1 National Research Laboratory of Vascular Biology and Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea, Republic of; 2 Graduate School of Nanoscience & Technology (WCU), Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea, Republic of; 3 Department of Physiology, College of Medicine Chungnam National University, Daejeon, Korea, Republic of; 4 Graduate School of Medical Sciences and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea, Republic of * Corresponding author; email: gykoh{at}kaist.ac.kr Abstract The stromal vascular fraction (SVF) in adipose tissue contains a pool of various stem and progenitor cells, but the existence of hematopoietic stem and progenitor cells (HSPCs) in the SVF has not been seriously considered. We detected the presence of HSPCs in the SVF by phenotypically probing with Lin-Sca-1+c-kit+ (LSK) and functionally confirming the presence using colony-forming cell assay and assessing the long-term multilineage reconstitution ability after SVF transplantation. The LSK population in the SVF was 0.004±0.001%, and 5x105 freshly isolated SVF cells gave rise to 13±4 multilineage colonies. Also, 0.15±0.03% of SVF cells was home to bone marrow (BM), especially near vascular and endosteal regions, 24 hours after blood transplantation. SVF transplantation was capable of generating a long-term (>16 weeks), but variable extent (2.1-32.1%) multilineage reconstitution in primary recipients, which was subsequently transferred to the secondary recipients by BM transplantation. All HSPCs within the SVF originated from the BM. Furthermore, the G-CSF mobilization of HSPCs from BM markedly elevated the number of phenotypic and functional HSPCs in the SVF, which induced a high efficiency long-term reconstitution in multilineage hematopoiesis in vivo. Our results provide compelling evidence that adipose tissue is a novel extramedullary tissue possessing phenotypic and functional HSPCs. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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