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Blood First Edition Paper, prepublished online November 4, 2009; DOI 10.1182/blood-2009-07-232322. This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Google Scholar Articles by Kobayashi, I. Articles by Suda, T. PubMed PubMed Citation Articles by Kobayashi, I. Articles by Suda, T. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 14, 2009; accepted October 5, 2009. Comparative gene expression analysis of zebrafish and mammals identifies common regulators in hematopoietic stem cells Isao Kobayashi1,3, Hiromasa Ono2, Tadaaki Moritomo3, Koichiro Kano2, Teruyuki Nakanishi3 and Toshio Suda1 1 Department of Cell Differentiation, The Sakaguchi Laboratory, School of Medicine, Keio University, Shinjuku-ku, Tokyo, Japan; 2 Laboratory of Cell and Tissue Biology, Graduate School of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan; 3 Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan * Corresponding author; email: kobaisao{at}sc.itc.keio.ac.jp Abstract Hematopoiesis in teleost fish is maintained in the kidney. We previously reported that Hoechst dye efflux activity of hematopoietic stem cells (HSCs) is highly conserved in vertebrates, and that Hoechst can be used to purify HSCs from teleost kidneys. Regulatory molecules that are strongly associated with HSC activity may also be conserved in vertebrates. Here, we identified evolutionarily conserved molecular components in HSCs by comparing the gene expression profiles of zebrafish, murine, and human HSCs. Microarray data of zebrafish kidney side population cells (zSPs) showed that genes involved in "cell junction" and "signal transduction" tended to be up-regulated in zSPs, while genes involved in "DNA replication" tended to be down-regulated. These properties of zSPs were similar to those of mammalian HSCs. Overlapping gene expression analysis showed that 40 genes were commonly up-regulated in these three HSCs. Some of these genes, such as egr1, gata2, and id1, have been previously implicated in the regulation of HSCs. In situ hybridization in zebrafish kidney revealed that expression domains of egr1, gata2, and id1 overlapped with that of abcg2a, a marker for zSPs. These results suggest that the overlapping genes identified here are regulated in HSCs and play important roles in their functions. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

	Copyright © 2009 by American Society of Hematology         Online ISSN: 1528-0020