Supplemental materials for: Lamouille et al, Vol 100, Issue 13, 4495-4501.

Supplemental Videos:

Comparison of the paths of migration of ALK-1QD�expressing cells and -gal�infected cells. Human microvascular endothelial cells were infected with Adb-gal or AdALK-1QD (MOI of 10) for 15 hours. The cell monolayers were then wounded with a plastic pipet tip and placed back at 37^�C in a CO₂ incubator. Closure of the wound was followed by time-lapse microscopy with pictures taken every 30 minutes for 48 hours, recorded using an Axiovert Zeiss 135M microscope (Goettingen, Germany). Note that, while -gal�infected cells have a relatively direct trajectory toward the center of the wound, ALK-1QD�expressing cells move in an unorientated manner, and many of them detach shortly after wounding.

MOV video 1 (4.3 MB): Closure of a wound in -gal-infected cells (control).

MOV video 2 (4.3 MB): Absence of closure of a wound in ALK-1QD-infected cells.