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Prepublished online as a Blood First Edition Paper on July 12, 2002; DOI 10.1182/blood-2001-12-0235.

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Blood, 1 November 2002, Vol. 100, No. 9, pp. 3164-3174

HEMATOPOIESIS

Identification of primary structural features that define the differential actions of IL-3 and GM-CSF receptors

Caroline A. Evans, Shahrul Ariffin, Andrew Pierce, and Anthony D. Whetton

From the Leukaemia Research Fund Cellular Development Unit, Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology (UMIST), Manchester, United Kingdom


    Abstract
Top
Abstract
Introduction
Materials and methods
Results
Discussion
References

Activation of human interleukin 3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) receptors, ectopically expressed in FDCP-mix multipotent cells, stimulates self-renewal or myeloid differentiation, respectively. These receptors are composed of unique alpha  subunits that interact with common beta c subunits. A chimeric receptor (hGM/beta c), comprising the extracellular domain of the hGM-CSF receptor alpha  subunit (hGM Ralpha ) fused to the intracellular domain of hbeta c, was generated to determine whether hbeta c activation is alone sufficient to promote differentiation. hGM-CSF activation of hGM/beta c, expressed in the presence and absence of the hbeta c subunit, promoted maintenance of primitive phenotype. This indicates that the cytosolic domain of the hGM Ralpha chain is required for differentiation mediated by activation of the hGM Ralpha , beta c receptor complex. We have previously demonstrated that the alpha  cytosolic domain confers signal specificity for IL-3 and GM-CSF receptors. Bioinformatic analysis of the IL-3 Ralpha and GM Ralpha subunits identified a tripeptide sequence, adjacent to the conserved proline-rich domain, which was potentially a key difference between them. Cross-exchange of the equivalent tripeptides between the alpha  subunits altered receptor function compared to the wild-type receptors. Both the mutant and the corresponding wild-type receptors promoted survival and proliferation in the short-term but had distinct effects on developmental outcome. The mutated hGM Ralpha promoted long-term proliferation and maintenance of primitive cell morphology, whereas cytokine activation of the corresponding hIL-3 Ralpha mutant promoted myeloid differentiation. We have thus identified a region of the alpha  cytosolic domain that is of critical importance for defining receptor specificity. (Blood. 2002;100:3164-3174)

© 2002 by The American Society of Hematology.

    Introduction
Top
Abstract
Introduction
Materials and methods
Results
Discussion
References

Hematopoietic stem and progenitor cells can undergo self-renewal or commitment to lineage-specific differentiation. In the stem cell compartment, the basis for the decision to self-renew or differentiate is complex and still poorly understood. Recent data on the PAX-5 protein suggest that transcription factors can suppress certain developmental options.1 Several other transcription factors have been shown to be critical for hemopoietic development on the basis of the effects of their overexpression or deletion.2,3 These include PU.1, SCL, and GATA 1-3.4-8 Cytokines regulate the activity of transcription factors, via interaction with specific receptors, and can influence the developmental fate of hematopoietic stem and progenitor cells.2 For example, interleukin 3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) bind to type I cytokine receptors composed of alpha  and beta c subunits. These receptors are expressed on a range of hematopoietic progenitor cells and their activation is associated with myeloid development.9,10 The GM-CSF receptor (GM-CSF R) can specifically instruct for myeloid differentiation.11 The IL-3 receptor (IL-3 R) is expressed earlier in myeloid cell development than the GM-CSF R, promotes expansion of CD34+ progenitor cell populations to a greater extent than GM-CSF, and has the ability to promote development of a greater range of lineages than GM-CSF.9,12 The alpha  subunits bind IL-3 or GM-CSF with low affinity and interact with a common beta  subunit (beta c) to form a functional high-affinity receptor complex. IL-3 and GM-CSF cannot bind to the beta c subunit independently of the alpha  subunits that are unique to each receptor. The beta c subunit is believed to elicit many of the signaling events emanating from these receptors.13,14

Knock-in mutations of the GM-CSF R reveal a complex regulation of hematopoiesis in vivo by the alpha  and beta c subunits.15-17 Furthermore, in vitro studies demonstrate that there are distinct differences in the signals emanating from the GM-CSF, IL-3, and IL-5 receptor complexes.18-20 We have previously demonstrated that the alpha  subunits act in combination with the hbeta c to govern developmental fate.18 Here, we further analyze the role of the beta c subunit and the cytosolic domain of the human GM-CSF receptor alpha  (hGM Ralpha ) subunit in developmental regulation using the murine FDCP-mix cell line as a model system.

Ectopic expression of wild-type and mutant human IL-3 receptor (hIL-3 R) and granulocyte-macrophage receptor (GM R) in the murine hematopoietic cell line, FDCP-mix, facilitates their analysis in the context of a multipotent hematopoietic progenitor cell line and allows an unambiguous description of their biologic effects. IL-3 and GM-CSF are species specific; thus, hIL-3 and hGM-CSF selectively activate the transfected human IL-3 and GM-CSF receptors. Our previous data have shown that coexpression of hGM Ralpha and hbeta c in murine FDCP-mix cells promotes myeloid differentiation, whereas the hIL-3 Ralpha , hbeta c promotes maintenance of multipotential cells. Furthermore, transfection and activation of a hIL-3 Ralpha (extracellular domain), hGM Ralpha , (cytosolic domain) chimeric receptor demonstrated that signal specificity resided in the cytosolic domain.18 The features of the hIL-3 and hGM receptors that signal for self-renewal and differentiation are further characterized by analysis of the function of receptor mutants in FDCP-mix cells.


    Materials and methods
Top
Abstract
Introduction
Materials and methods
Results
Discussion
References

The FDCP-mix cell line

The FDCP-mix (clone A4) cells were routinely cultured in Fischer medium supplemented with 5% (vol/vol) medium conditioned by the X63-Ag-653 cell line (used as a source of murine IL-3),21 20% (vol/vol) horse serum. For granulocyte-macrophage differentiation (G/M Diff) cells were cultured in Iscove modified Dulbecco medium supplemented with fetal calf serum (20% vol/vol), recombinant murine (rm) GM-CSF (5 ng/mL), rmG-CSF (5 ng/mL), and rmIL-3 (0.1 ng/mL), as previously described.22 The effects of activation of hIL-3 R or hGM R were assessed by culturing the receptor transfects with hGM-CSF or hIL-3, respectively, as the single cytokine present. They were thus studied in the absence of murine cytokines. Murine cytokines were supplied by Calbiochem (Nottingham, United Kingdom). Recombinant hIL-3 and hGM-CSF were gifts from Sandoz Pharma (Basel, Switzerland) and Glaxo (Greenford, United Kingdom), respectively, as previously described.18

Transfection of FDCP-mix cells with hIL-3 and hGM-CSF receptor mutants

Retroviral transfections were performed using the pM5 vector containing the receptor subunit gene and an antibiotic-resistance gene as a selectable marker.18 Neomycin phosphotransferase (neo) and hygromycin phosphatases (hgr) were used to select for the mutated receptor alpha  subunits and hbeta c, respectively. The chimeric receptor alpha  subunit was produced by introducing a NheI restriction site (position 330 for the hGM Ralpha and 446 for the hbeta c) into the transmembrane domain by site-directed mutagenesis. The mutagenesis was carried out on dsDNA using the Chameleon ds mutagenesis kit (Stratagene, Amsterdam, The Netherlands) in accordance with the manufacturer's instructions. The mutant hGM Ralpha (hGM (M) Ralpha ) and the corresponding hIL-3 (M) Ralpha were generated by site-directed mutagenesis using the Quickchange mutagenesis kit (Stratagene). All mutations and constructs were confirmed by DNA sequencing. The receptor constructs were transfected into the Phoenix fibroblast packaging cell line23 using calcium phosphate precipitation (Gibco, Paisley, United Kingdom). Retroviral transfection was performed by culturing FDCP-mix cells in viral supernatant for 48 hours. FDCP-mix cells were harvested, washed, and selected for antibiotic resistance by culturing in medium supplemented with 1 mg/mL G418 or 0.15 mg/mL hygromycin B (or both) as appropriate. Polyclonal cell populations were labeled for flow cytometric analysis using antibodies directed against the extracellular domain of the receptor subunits (see below). Cells were sorted on the basis of receptor subunit expression using the cell sorting facility of the FACS Vantage flow cytometer and 3 polyclonal populations obtained for analysis (Becton Dickinson, Oxford, United Kingdom). As evaluated by flow cytometry, the expression level of mutant receptors was found to be similar in the populations generated and equivalent to more than 10 000 receptors/cell.

Analysis of hGM-CSF R and hIL-3 R subunit expression

Ectopic receptor expression was confirmed by flow cytometry using a 2-step antibody labeling procedure as previously described.18

Measurement of proliferation

DNA synthesis was used as a measure of proliferation and was performed by determining incorporation of [3H]-thymidine as previously described.24 Briefly, cells were washed and incubated with cytokines (5 × 104 cells/sample) for 16 hours. Samples were then pulsed for 4 hours with 10 µCi/mL [3H]-thymidine (0.37 MBq), harvested using a Hewlett Packard Top Count cell harvester and the incorporated radioactivity measured by scintillation counting.

Morphologic analysis

Morphologic analysis of cells in liquid culture was performed as described.25 Slides were prepared using a Shandon cytospin centrifuge and stained with May-Grünwald-Giemsa stain. At least 100 cells were scored for each slide.

Analysis of differentiation markers

Cell surface expression of Sca-1, Gr-1, and Mac-1 (CD11b) differentiation markers were analyzed by flow cytometry as previously described.18


    Results
Top
Abstract
Introduction
Materials and methods
Results
Discussion
References

Generation of hIL-3 R and hGM R mutants

To further characterize the role of the alpha  and beta c subunits in hGM-CSF and hIL-3 receptor-mediated signaling, we have constructed and evaluated the function of 2 types of mutant GM R and hIL-3 R. A schematic representation of the mutants tested is shown in Figures 1 and 2. The chimeric hGM/beta c receptor is composed of the extracellular domain of the alpha  subunit and a beta c cytosolic domain (Figure 1). This was used to study the biologic response elicited by activation of hbeta c in the absence of the alpha  cytosolic domain. The hIL-3 (M) Ralpha and hGM (M) Ralpha mutants were generated based on sequence comparison of the alpha  cytosolic domains that indicated unique tripeptide sequences, PIG and KLN, in IL-3 Ralpha and GM Ralpha , respectively (shown as underlined sequences in Figure 2). These tripeptides were different between the receptors but conserved across species and therefore postulated to perhaps play a role in conferring receptor specificity. For the hIL-3 (M) Ralpha , the tripeptide PIG344-346 was replaced with the corresponding KLN365-367 tripeptide from the hGM Ralpha subunit using site-directed mutagenesis. Replacing the KLN sequence with PIG of hIL-3 Ralpha generated the corresponding mutant hGM Ralpha , designated hGM (M) Ralpha (Figure 2).


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Figure 1. Sequence of transmembrane region of the hGM/beta c chimera. The transmembrane domain sequence of the chimeric hGM/beta c receptor mutant is shown with the adjacent amino acids. The sequences from wild-type hGM Ralpha and hbeta c subunits are shown in plain and bold text, respectively. The putative transmembrane region is underlined and is based on sequence data for the hGM Ralpha and h/beta c.51,52



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Figure 2. Sequence comparison of cytosolic domains of hGM Ralpha , IL -3 Ralpha , and IL-5 Ralpha . Aligned cytosolic sequences of hGM Ralpha , IL-3 Ralpha , and the related IL-5 Ralpha are shown. Amino acids present in all 3 Ralpha subunits or that are subject to conservative change are shown in bold. Those conserved between species are boxed and those present in 2 alpha  subunits are marked with an asterisk (*). The PIG and KLN sequences of hIL-3 Ralpha and hGM Ralpha are indicated by underlining.

FDCP-mix cells are nonleukemic, karyotypically normal and their survival, proliferation, and development are subject to regulation by cytokines.26,27 The cell line is maintained in murine IL-3, in which cells maintain a primitive phenotype and multipotential differentiation capacity. Cells were cultured in murine IL-3 during transfection and antibiotic selection of cells to preserve the multipotential differentiation capacity. Cells were then analyzed for human receptor gene expression by flow cytometry, using antibodies directed against the extracellular domain of the hIL-3 and hGM R subunits. The cells maintained multipotency following transfection and selection (see below). Human cytokine receptor function was assessed in the panel of cell lines generated by determining the effect of culturing the cells with either hIL-3 or hGM-CSF as the only cytokine present.

The effects of chimeric hGM/beta c

To perform a complete analysis of the potential of beta c to promote myeloid differentiation, we determined the effects of expression of a chimeric receptor hGM/beta c expressed in the presence and absence of full-length hbeta c in FDCP-mix cells. The chimeric hGM/beta c receptor represents a "minimal" form of the receptor with the ligand-binding domain of the alpha  subunit and the cytosolic signaling domain of the hbeta c.

As previously reported, no specific fluorescence labeling of the parental FDCP-mix cells was detected with anti-hIL-3 Ralpha , anti-hGM Ralpha , or anti-hbeta c antibodies.18 Cells positive for hbeta c and hGM/beta c coexpression were identified and sorted by flow cytometry using anti-hGM Ralpha and anti-hbeta c antibodies, respectively, following antibiotic selection (Figure 3A). The expression profiles of hGM/beta c and hbeta c in a representative populations are shown in Figure 3, panels Ai and Aii, respectively, where a log increase in fluorescence was observed relative to the control. The level of receptor subunit expression was similar to that previously obtained when the hbeta c was coexpressed with the hGM Ralpha or hIL-3 Ralpha .18


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Figure 3. Effect of expression of hGMbeta c chimera with or without hbeta c in FDCP-mix cells. (A) Analysis of receptor subunit expression. Parental and hGM/beta c transfected cells were analyzed for expression of the extracellular domain of the (i) hGM Ralpha and (ii) hbeta c subunit using a 2-step labeling procedures and flow cytometry. The solid gray histogram represents the cell autofluorescence obtained in the absence of antibody staining. Representative histograms are shown for labeling obtained in the presence of secondary antibody only (gray) and in the presence of both primary and secondary antibody (black). (B) Effects on cellular proliferation. Cells expressing hGM/beta c (open circle ) and hGM/beta c, hbeta c () were cultured in hGM-CSF (1 ng/mL) for 14 days. Cellular viability was assessed at intervals and the results are expressed as viable cell number (× 105/mL) and are mean ± SEM from 3 experiments. The results obtained with the wild-type hGM Ralpha ,beta c are shown for comparison (). (C) Effect of activation of hGM/beta c expressed alone or in combination with the hbeta c subunit on cell morphology. (i) hGM/beta c cells, (ii) hGM/beta c, hbeta c, and (iii) wild-type hGM Ralpha , hbeta c were cultured in hGM-CSF (1 ng/mL) for 7 days and photomicrographs were prepared following May-Grünwald-Giemsa staining of cytospin preparations. The morphology of cells cultured in the presence of murine cytokines (G/M Diff conditions), which promote myeloid development, are shown for comparison for (iv) hGM/beta c cells and (v) hGM/beta c,beta c and (vi) wild-type hGM Ralpha , hbeta c. Results are shown from an experiment representative of 3. Bar is 10 µm.

The functional capability of beta c to promote self-renewal/differentiation in the absence of the alpha  subunit cytosolic domain was assessed and the results are shown in Figure 3, panels B and C. Cells expressing hGM/beta c alone or in combination with hbeta c survived and proliferated in response to hGM-CSF (at >=  0.01 ng/mL). There was significantly greater proliferation of cells coexpressing the hGM/beta c plus hbeta c compared to hGM/beta c alone in response to hGM-CSF (Figure 3B) and both could promote proliferation for more than 10 days, unlike the wild-type hGM Ralpha , hbeta c. These data suggest that the formation of higher order beta c complexes stimulates proliferation more effectively. Interestingly, chimeric receptors, composed of the extracellular and transmembrane domains of the hGM Ralpha (or IL-5 Ralpha ) and the intracellular domain of the hbeta c, have been shown to form functional oligomeric receptor complexes in combination with hbeta c.28-30

We compared the morphology of hGM-CSF-treated cells expressing hGM/beta c with or without hbeta c to cells expressing the wild-type hGM Ralpha , hbeta c. The results are shown in Figure 3, panel Ci-iii, and Table 1. Cells were cultured at a dose of 1 ng/mL since there is clear receptor activation at this dose and it avoids activation of endogenous mGM-CSF R subunit, which would occur at higher doses (100 ng/mL). The wild-type hGM Ralpha , hbeta c promotes formation of granulocytes and macrophages in response to hGM-CSF (Figure 3Ciii and Table 118). In contrast, the hGM/beta c with or without hbeta c cells cultured in hGM-CSF for 7 days exhibited primitive cell morphology, composed mainly of blast cells and early granulocytes. The cells, however, retained their ability to undergo differentiation into granulocytes and macrophages in response to murine cytokines (Figure 3Civ-vi). This infers that the hbeta c alone does not elicit a differentiation stimulus equivalent to that of wild-type hGM Ralpha , hbeta c and further supports our hypothesis that it is the cytosolic domain of the GM-CSF Ralpha that acts, in combination with the beta c, to promote differentiation.

                              
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Table 1. Effect of activation of hGM/beta c chimera with or without hbeta c on cell morphology

Bioinformatic analysis of the primary sequence of the IL-3 Ralpha and GM-CSF Ralpha subunits

The differences between the IL-3 Ralpha - and GM Ralpha -mediated maintenance of pluripotentiality and development signals lie in the cytosolic domain sequence18 and are potentially revealing about the molecular mechanisms of cytokine receptor activation. Sequence comparisons were made between the cytosolic domains of the alpha  subunits of the GM-CSF, IL-3, and IL-5 receptor subfamily (Figure 2). Areas of sequence that are divergent between receptor cytosolic domains but conserved between species were deemed to be important in the functional differences between the receptors. A further consideration was the potential existence of functional domains. There is no evidence of tyrosine or serine/threonine phosphorylation of the alpha  cytosolic domains and there are no apparent WW, SH3, or SH2 domains present. However, previous work on the alpha  subunit has shown that the first 29 cytosolic amino acids (from the membrane region) are sufficient for cell signaling, proliferation, and differentiation.31,32 This encompasses the proline-rich juxtamembrane region and adjacent amino acids. Figure 2 shows that there is cross-species and cross-receptor conservation of this region (QRLFP**P). However, there is a divergent region close to the proline-rich domain, which may be important in that a proline residue in the IL-3 Ralpha is replaced by lysine in the hGM Ralpha . Predictions of hydrophobicity and secondary structure revealed that the proline residue was likely to be exposed to the cytosol, making it a candidate for mediating differential signaling. Furthermore, proline residues have the potential to affect secondary structure by introducing kinks in the helices.33

We therefore exchanged the tripeptide KLN365-367 in GM Ralpha for PIG from the IL-3Ralpha sequence to generate a mutant hGM Ralpha subunit (hGM (M) Ralpha ). The corresponding hIL-3 Ralpha mutant was also generated for comparison (Figure 2). The aim was to define a region of the receptor that may regulate GM Ralpha -mediated differentiation.

Substitution of the PIG region of IL-3 Ralpha with the KLN sequence from GM Ralpha generates a receptor that promotes myeloid differentiation

FDCP-mix cells were cotransfected with the hIL-3 (M) Ralpha and hbeta c subunits and compared to the wild type hIL-3 Ralpha , hbeta c cells. The expression of the hIL-3 (M) Ralpha and hbeta c subunits was analyzed by flow cytometry (Figure 4Ai,ii). These expression levels were similar to those obtained for the wild-type hIL-3 R.18 We have previously determined that culture of wild-type receptor transfects with hIL-3 promoted maintenance of blast cell phenotype and proliferative potential.18 Addition of hIL-3 promoted survival and proliferation of both the wild-type and mutant hIL-3 R (Figure 4Bi,ii). However, whereas activation of the wild-type hIL-3 receptors promotes self-renewal (Figure 4Ci), addition of hIL-3 to hIL-3 (M) Ralpha , hbeta c cells generated cells with a much more differentiated phenotype (Table 2). The hIL-3 (M) Ralpha , hbeta c cells therefore more closely resembled the response of wild-type hGM R-expressing cells (Figure 4Cii, Table 118). The dose of hIL-3 used (0.1-100 ng/mL) did not influence the outcome observed when mutated IL-3 Ralpha , hbeta c was activated. In all cases mature myeloid cells were formed in a 7-day period. The hIL-3 (M) Ralpha , hbeta c cells show an increased expression of Gr-1 and Mac-1 compared to the wild-type cells in response to hIL-3 (Table 3). These data show that PIGright-arrowKLN substitution thus transforms a signal for proliferation and self-renewal into a differentiation signal.


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Figure 4. Effects of hIL-3 (M) Ralpha activation. (A) Cell surface expression. hIL-3 (M) Ralpha , hbeta c cell transfects were analyzed for expression of the extracellular domains of the (i) hIL-3 Ralpha and (ii) hbeta c by flow cytometry. Cells were sequentially incubated with anti-hIL-3 Ralpha antibody and fluorescein isothiocyanate (FITC)-conjugated antimouse secondary antibody. The solid gray histogram represents the cell autofluorescence obtained in the absence of antibody staining. Representative histograms are shown for labeling obtained in the presence of secondary antibody only (gray) and in the presence of both primary and secondary antibody (black). (B) Cell survival proliferation. Cells expressing wild-type hIL-3 Ralpha , hbeta c () or hIL-3 (M) Ralpha , hbeta c (black-square) were cultured in hIL-3 (0-100 ng/mL). (i) Cell viability was assessed at 48 hours using trypan blue. The results are expressed as cell viability (percent rmIL-3 response) and are mean values ± SEM from at least 3 experiments. (ii) [3H]-thymidine incorporation was assessed after 16 hours in culture. The results are expressed as percentage of the response obtained with rmIL-3 (10 ng/mL). (C) Cell morphology. Cells expressing (i) wild-type hIL-3 Ralpha , hbeta c or (ii) hIL-3 (M) Ralpha , hbeta c were cultured in hIL-3 (1 ng/mL) for 7 days. Photomicrographs were prepared from May-Grünwald-Giemsa-stained cytospin preparations. Results are representative of 3 experiments. Bar is 10 µm.


                              
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Table 2. Effect of activation of hIL-3 (M) Ralpha , hbeta c compared to wild-type hIL-3 Ralpha , hbeta c on cell morphology


                              
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Table 3. Effect of activation of hIL-3 (M) Ralpha , hbeta c compared to wild-type hIL-3 Ralpha , hbeta c on expression of differentiation markers

Effect of reciprocal KLNright-arrowPIG substitution on hGM R function

A key question arising from these results obtained is the nature of the effect of the reciprocal substitution of the KLN tripeptide in the hGM Ralpha cytosolic domain with the PIG tripeptide region from the hIL-3 Ralpha . If this region is of critical importance, it would be predicted that such a mutant hGM receptor would exhibit a perturbation of the hGM-CSF-mediated differentiation response. Retroviral vectors containing the mutant hGM (M) Ralpha with KLN365-367 replaced by PIG and the hbeta c were used to generate cotransfected populations of FDCP-mix cells expressing both the GM (M) Ralpha and hbeta c subunits. A profile of receptor subunit expression as determined by flow cytometry is shown in Figure 5A. The expression levels detected by anti-hGM Ralpha and hbeta c antibodies were similar to those previously obtained for the hGM/beta c, hbeta c transfects (Figure 3A) and also the wild-type receptor.18


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Figure 5. Coexpression of hGM (M) Ralpha and hbeta c in FDCP-mix cells. (A) Cell surface expression. Cells transfected with hGM (M) Ralpha in combination with hbeta c cells were analyzed for expression of the extracellular domains of the hGM Ralpha and hbeta c by flow cytometry. Cells were sequentially incubated with (i) anti-hGM Ralpha antibody or (ii) anti-hbeta c antibody and FITC-conjugated antimouse secondary antibody. The solid gray histogram represents the cell autofluorescence obtained in the absence of antibody staining. Representative histograms are shown for labeling obtained in the presence of secondary antibody only (gray) and in the presence of both primary and secondary antibody (black). (B) Effects on cell survival. Cells coexpressing hGM (M) Ralpha , hbeta c () were cultured in the presence of hGM-CSF (0-100 ng/mL) for 48 hours prior to assessment of cell viability based on trypan blue exclusion. The data obtained for the chimeric hGM/beta c, hbeta c (black-square) are shown for comparison. Results are expressed as a percentage of the rmIL-3 (10 ng/mL) response and are mean values ± SEM of 3 experiments. (C) Long-term proliferation. Cells coexpressing either hGM/beta c, hbeta c () or hGM (M) Ralpha , hbeta c (black-triangle) were cultured for 60 days in the presence of hGM-CSF (1 ng/mL). The growth rate was determined from the initial and subsequent cultures of cells seeded at 1 × 105/mL and resuspended in fresh media when the cell number was more than 5 × 105/mL. The results are expressed as log viable cell number/mL. The growth rates of hGM/beta c, hbeta c () and hGM (M) Ralpha , hbeta c () cells in response to rmIL-3 (10 ng/mL) are also shown. Results are mean ± SEM of 3 experiments.

Effects of coexpression of hGM (M) Ralpha and hbeta c were determined by culturing cells in the presence of hGM-CSF. Cell viability, proliferation, and differentiation were assessed and compared to those obtained by activation of the hGM/beta c, hbeta c receptor. Both types of mutant receptor promoted cell survival and proliferation (Figure 5B,C). In contrast to the wild-type hGM Ralpha ,beta c receptor, however, the hGM (M) Ralpha , hbeta c and hGM/beta c, hbeta c promoted the long-term proliferation of FDCP-mix cells, which were continuously cultured in hGM-CSF for a period of 60 days (Figure 5C).

The morphology of the cells was assessed at intervals during this time period and the cells maintained a primitive morphology, with the cultures being composed of mainly blasts and early granulocytes (Figure 6Ai,ii and Bi,ii). The dose of hGM-CSF (0.1-100 ng/mL) did not influence the outcome observed when mutated GM R was activated. The cells remained with a blast cell or early granulocytic morphology over the time course of the experiment. They did not acquire factor independence during this time as they remained responsive to mIL-3 and hGM-CSF, undergoing apoptosis following cytokine removal (data not shown). The differentiation potential of the cells was maintained because cells washed free of hGM-CSF after 60 days differentiated into granulocytes and macrophages after 7 days of culture with murine cytokines, which promote G/M Diff (Figure 6Biii,iv).


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Figure 6. Effects of activation of hGM (M) Ralpha , hbeta c and hGM/beta c, hbeta c on cell development. (A) Cell morphology. (i) hGM (M) Ralpha , hbeta c and (ii) hGM/beta c, hbeta c cells were cultured in hGM-CSF (1 ng/mL) for 60 days and cytospin samples prepared at intervals during this time. Morphology was assessed following May-Grünwald-Giemsa staining. The morphology results are expressed as percentage of the total cells scored and pooled data from 3 experiments. The SEMs were less than 10%. (B) Ability to differentiate following long-term culture in hGM-CSF. Photomicrographs are shown of (i) hGM (M) Ralpha , hbeta c cells and (ii) hGM/beta c, hbeta c cells cultured in hGM-CSF (1 ng/mL) for 60 days. After this time, (iii) hGM (M) Ralpha , hbeta c cells and (iv) hGM/beta c, hbeta c cells were harvested, washed, and cultured in murine cytokines that promote myeloid development for a further 7 days. Cytospin preparations were May-Grünwald-Giemsa stained and are representative of 3 separate experiments. Bar is 10 µm.

These data demonstrate that the PIGright-arrowKLN mutation fundamentally alters the response to hGM-CSF compared to wild-type hGM Ralpha , beta c. Differentiation status was also assessed by the expression of cell surface maturation markers. Expression levels of stem cell antigen-1 (Sca-1, Ly-6) and mature myeloid marker antigens recognized by Gr-1 (Ly6/G) and Mac-1 were measured by flow cytometry. At day 7, control cultures of the hGM R mutants in murine cytokines, which promote G/M Diff, expressed increased levels of Gr-1 and Mac-1 with the level of Sca-1 being decreased (hGM/beta c, hbeta c) or similar (hGM (M) Ralpha , hbeta c) to that obtained for cells cultured in murine IL-3 (Figure 7A,B). Wild-type hGM Ralpha , beta c cells increase expression of the differentiation markers Gr-1 and Mac-1 but not Sca-118 (Table 4). This is consistent with myeloid differentiation. For the hGM/beta c, hbeta c and hGM (M) alpha , hbeta c cells cultured with hGM-CSF the situation with respect to expression of biochemical markers of differentiation was more complex. While these cells were primitive in terms of morphology and proliferative potential, there was increased expression of not only a primitive marker, Sca-1, but also the differentiation marker, Mac-1, at day 7. Interestingly, cells expressing hIL-3 Ralpha , hbeta c do not show changes in differentiation marker expression and undergo self-renewal (Tables 3 and 4). The results obtained for the GM R mutants indicate that there is uncoupling of the regulation of differentiation marker expression from clonal suppression that is regulated by the wild-type hGM R. 


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Figure 7. hGM-CSF mediated changes in expression of differentiation markers of cells expressing mutant hGM-CSF receptors. Cells expressing (A) hGM (M) Ralpha , hbeta c and (B) hGM/beta c, hbeta c cells were cultured in 1 ng/mL hGM-CSF for 7 days. The levels of expression of the differentiation markers are shown as representative histograms from at least 3 experiments (____). Nonspecific labeling was determined using the corresponding isotype control (- - -). The results obtained for cells cultured in murine cytokines that promote G/M Diff for 7 days are also shown for comparison.


                              
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Table 4. Effects of activation of wild-type and mutant hIL-3 and hGM-CSF receptors in FDCP-mix cells

Cells washed to remove the cytokine after 60 days, and subsequently cultured with murine cytokines that promote G/M Diff, differentiated morphologically into granulocytes and macrophages (Figure 6Biii,iv) and showed decreased levels of Sca-1 and increased levels of Gr-1 and Mac-1 (Figure 8A,B). This confirms the maintenance of differentiation potential when cultured in hGM-CSF, even after a prolonged period. Culture of hGM (M) Ralpha , hbeta c cells and hGM/beta c, hbeta c cells with hGM-CSF resulted in continued perturbation of differentiation marker expression; in particular, there was increased Sca-1 expression relative to time-matched controls of cells cultured in murine IL-3, the cytokine in which cells are maintained routinely.


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Figure 8. hGM-CSF mediated changes in expression of differentiation markers of cells expressing mutant hGM-CSF receptors. Cells expressing (A) hGM (M) Ralpha , hbeta c and (B) hGM/beta c, hbeta c cells were cultured in 1 ng/mL hGM-CSF for 60 days. The levels of expression of the differentiation markers are shown as representative histograms from at least 3 experiments (____). Nonspecific labeling was determined using the corresponding isotype control (- - -). The results obtained for cells cultured in murine cytokines that promote G/M Diff for 7 days after 60 days culture in hGM-CSF are also shown.

The data obtained for the wild-type and mutant hIL-3 and hGM-CSF receptors after 7 days in culture are summarized in Table 4.


    Discussion
Top
Abstract
Introduction
Materials and methods
Results
Discussion
References

Analysis of the role of the cytosolic domain of the hGM Ralpha and the functional capability of the hbeta c subunit

The GM-CSF receptor consists of a ligand-specific alpha  subunit and the beta c subunit that is shared in common with the IL-3 and IL-5 receptors. The beta c subunit becomes tyrosine phosphorylated on receptor activation, and beta c has been proposed to be the primary signal transduction protein in the receptor alpha , beta c subunit complex.34,35 However, our data, and those of others, shows the alpha  subunit cytosolic domains are essential for receptor function and stimulate different signaling pathways.18,19,28,31,32,36,37 More important, perhaps, is the fact that these alpha  subunits can influence differentiation.18 However, the significance of beta c cannot be understated. Constitutively activated beta c can promote myeloproliferative disorders when expressed in marrow reconstitution systems or transgenic mice.38,39 It is not clear, however, if activation of beta c alone is sufficient to promote myeloid cell development.

Experiments with a chimeric hGM/beta c receptor transgenic mouse have addressed this question.17 This receptor was composed of the extracellular and transmembrane domain of the alpha  subunit and the cytoplasmic domain of hbeta c. Administration of hGM-CSF to these mice results in a phenotype equivalent to that of transgenic mice expressing wild-type hGM-CSF receptor in which white blood cell counts are elevated and splenomegaly is observed. The cytoplasmic region of the alpha  subunit would, thus, appear to be functionally redundant and it was proposed that the box 1 region of the cytosolic domain of beta c functionally compensates for the lack of the alpha  cytosolic