|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
Prepublished online as a Blood First Edition Paper on October 3, 2002; DOI 10.1182/blood-2002-05-1459.
CLINICAL OBSERVATIONS, INTERVENTIONS, AND THERAPEUTIC TRIALS
From the Institute of Hematology, Erasmus Medical
Centre, Rotterdam, Netherlands; the Department of Cell Biology and
Genetics, Erasmus Medical Centre, Rotterdam, Netherlands;
the Department of Clinical Genetics, Erasmus Medical Centre, Rotterdam,
Netherlands; the Department of Hematology, Vrije
Universiteit Medical Centre, Amsterdam, Netherlands; the
Department of Hematology, University Medical Centre, Utrecht,
Netherlands; and the Department of Hematology, University
Hospitals, Katholieke Universiteit Leuven, Leuven,
Belgium.
The proto-oncogene EVI1 encodes a DNA binding protein
and is located on chromosome 3q26. The gene is aberrantly expressed in
acute myeloid leukemia (AML) patients carrying 3q26 abnormalities. Two
mRNAs are transcribed from this locus: EVI1 and a fusion of EVI1 with MDS1
(MDS1-EVI1), a gene located 5' of
EVI1. The purpose of this study was to investigate which of
the 2 gene products is involved in transformation in human AML. To
discriminate between EVI1 and
MDS1-EVI1 transcripts, distinct real-time
quantitative polymerase chain reaction (PCR) assays were
developed. Patients with 3q26 abnormalities often showed high
EVI1 and MDS1-EVI1 expression. In a
cohort of 319 AML patients, 4 subgroups could be distinguished: EVI1+ and
MDS1-EVI1 The EVI1 proto-oncogene is located on
human chromosome 3q26 and is involved in pathogenesis of human acute
myeloid leukemia (AML) or myelodysplastic syndrome (MDS) carrying 3q26
rearrangements.1,2 Although these rearrangements are
infrequent in AML, they are of remarkable prognostic value. Patients
with these karyotypes often do not respond to therapy, even when the
most active antileukemic therapeutic options are
used.3
EVI1 encodes a nuclear DNA binding protein with 2 zinc
finger domains, an N-terminal domain containing 7 zinc fingers and a
more C-terminal domain with 3 zinc fingers. Both domains recognize and
bind to specific DNA consensus sequences.4,5 While most reports indicate that EVI1 gene expression is not detectable
in normal blood or bone marrow,6-9 other studies suggest
low but detectable expression of EVI1 in normal bone marrow
cells.10 High expression of EVI1 has been
observed in developing oocytes and in the kidney.11
Although the exact mechanism of transformation by EVI1 is
still obscure, several studies have shown that inappropriate expression
of EVI1 in immature hematopoietic cells interferes with
erythroid and granulocytic development.12 It has become evident that EVI1 may form a fusion transcript with the
MDS1 gene. MDS1 is a 4-exon gene located upstream
of EVI1. Splicing may occur from exon 2 of MDS1
to the second exon of EVI1 to form the fusion transcript
MDS1-EVI1. This intergenic splicing may occur
in normal tissues as well as in myeloid
leukemia.1,13 MDS1-EVI1 encodes a
longer protein containing the entire EVI1 protein but with an additional, unique N-terminal extension. Although related, the 2 proteins EVI1 and MDS1-EVI1 may have opposite
properties.14,15
Previous studies showed that EVI1 may be expressed in
patients without 3q268,9,16,17; however, the sets of
polymerase chain reaction (PCR) primers that were chosen in
these studies did not discriminate between EVI1 and
MDS1-EVI1. We designed different primer and probe
combinations to discriminate between EVI1 and MDS-EVI1 and quantify the transcript levels by means of
real-time PCR analysis. To provide an answer to the question, which of
these transcripts are expressed in patients with 3q26 rearrangements, we first screened 7 patients carrying 3q26 abnormalities. Using the
same technique, we studied the expression levels of these transcripts
in the bone marrow samples of healthy volunteers. To investigate how
frequently EVI1, MDS1-EVI1, or
MDS1 may be expressed in de novo AML, we determined
expression levels of these transcripts in a cohort of 319 AML patients
at diagnosis. The results were analyzed in relation to hematologic,
cytogenetic, and clinical characteristics as well as outcome of
therapy. Our data demonstrate that expression of EVI1 and
not of MDS1-EVI1 is associated with highly
aggressive AML. High EVI1 expression occurs with high
frequency in patients without 3q26 abnormalities, suggesting other
mechanisms of aberrant EVI1 expression.
Patients and healthy volunteers
RNA isolation, cDNA synthesis, and real-time PCR
An aliquot of one 20th of the resulting cDNA was used for quantitative
PCR amplification. Real-time PCR amplification was performed with the
ABI PRISM 7700 Sequence Detector (Applied Biosystems, Nieuwerkerk aan
den IJssel, Netherlands), using 50 µL mix containing 2 µL cDNA
sample; 250 µM deoxyribonucleoside triphosphates (dNTPs; Amersham Pharmacia Biotech, Roosendaal, Netherlands); 15 pmol forward
and reverse primer (Life Technologies); 3 mM MgCl2 (5 mM
for porphobilinogen deaminase [PBGD] reaction); 200 nM
probe, labeled at the 5' end with the reporter dye molecule FAM
(6-carboxy-fluorescein) for EVI1,
MDS1-EVI1, and MDS1 or with JOE
(carboxyrhodamine) for PBGD and at the 3' end with the quencher dye
molecule TAMRA (6-carboxy-tetramethyl-rhodamine; Eurogentec,
Maastricht, Netherlands); 5 µL 10 × buffer A; 30 µL water; and
1.25 U AmpliTaq Gold (Applied Biosystems). The thermal cycling
conditions included 10 minutes at 95°C followed by 45 cycles of
denaturation for 15 seconds at 95°C and annealing/extension at 60°C
for 30 seconds. The primer/probe combinations were chosen such that we
could discriminate between EVI1,
MDS1/EVI1, and MDS1 transcripts
(Figure 1). The oligonucleotide sequences
of the primers and probes are shown in Table
2.
Using 7 different dilutions of a cDNA sample (equal to 0.0064-100 ng
total RNA) prepared from AML cells that were positive for each of the
transcripts (patient 2, Table 3),
standard curves were made for EVI1 and
MDS1-EVI1. As the expression levels of MDS1 transcripts were too low to measure the efficiency of
amplification, MDS1 expression was considered positive (+)
when the threshold cycle (Ct) value was below 35.
To determine the expression levels in AML, all samples were tested in
duplicate and the average values were used for quantification. To
quantify the relative expression of EVI1 and
MDS1-EVI1, the Ct values were normalized for
endogenous reference
(
To define high EVI1 and MDS1-EVI1
expression, a cutoff value of 50 (relative expression
2 Northern blotting Northern blotting was carried out on mRNA isolated from healthy bone marrow samples as well as AML samples. A portion (20 µg) of total RNA from each sample was separated on a 1% agarose, 6% formaldehyde gel and blotted with 10 × SSC (sodium chloride/sodium citrate; Amersham) onto Hybond-N+ nylon membrane (Amersham). The blot was hybridized in 1N NaH2PO4 buffer containing 7% sodium dextran sulfate and 1 mM EDTA (ethylenediaminetetraacetic acid), pH 8.0. As probe, human EVI1 (600-bp HindIII-Ncol fragment) and murine GAPDH (777-bp HindIII-EcoRI fragment)23 were 32P-labeled by random priming (Boehringer, Mannheim, Germany). The blot was hybridized at 65°C overnight and washed for 15 minutes at 65°C in 2 × SSC/0.5% sodium dodecyl sulfate (SDS) and for 15 minutes at 65°C in 1 × SSC/0.5% SDS. It was then analyzed by autoradiography.Cytogenetic analysis and stratification according to karyotype risk group Cytogenetic analysis was carried out according to standard techniques, and the abnormalities were categorized in 3 cytogenetic groups. Patients with inv(16)/t(16;16), t(8;21), and t(15;17) abnormalities were considered as being in the favorable-risk category. The unfavorable-risk category was defined by the presence of 5/del(5q), 7del(7q), t(6;9), t(9;22), 3q26 abnormality or complex
karyotype (more than 3 abnormalities). All other patients were
classified as intermediate risk. Karyotypes were described
according to the International System for Human Cytogenetic
Nomenclature.22
Analysis of FLT3 internal tandem duplication mutations in AML The internal tandem duplications in exon 11 of the human FLT3 gene were determined as described previously.24 Briefly, cDNA (derived from 50 ng total RNA) and genomic DNA (1 µg) were subjected to PCR using primers 11F 5'-CAATTTAGGTAT-3' and 11R 5'-CAAACTCTAAATTTTCTCT-3'. The PCR cycling conditions were as follows: 3 minutes at 94°C; 30 cycles of 1 minute at 94°C, 1 minute at 54°C, 1 minute at 72°C; and a final step of 10 minutes at 72°C. PCR products were resolved on a 2.5% agarose gel.Statistical analysis Statistical analysis was performed with Stata Statistical Software, Release 7.0 (Stata, College Station, TX). Spearman rank correlation, Pearson 2 test, and Kruskal-Wallis test
were used to assess the association between EVI1 and
MDS1-EVI1 expression and the clinical and
hematologic characteristics of patients. Actuarial probabilities of
overall survival (OS, with failure death due to any cause) and
event-free survival (EFS, with failure in case of no complete remission
(CR) at day 1 or at relapse or at death in first CR) were
estimated by the method of Kaplan and Meier. The Cox proportional
hazards model was applied to determine the association of high
EVI1 expression with OS and EFS, without and with adjustment
for other factors such as age, cytogenetic risk, and FLT3 internal
tandem duplication (FLT3-ITD). All tests were 2-sided, and a
P of less than .05 was considered statistically significant.
Quantification of EVI1, MDS1-EVI1, and MDS1 by real-time PCR EVI1, MDS1-EVI1, and MDS1 expression levels were analyzed by real-time PCR employing specific primer/probe combinations (Figure 1; Table 2). Efficiency of the quantification method for EVI1 and MDS1-EVI1 was examined by standard curves made using mRNA isolated from AML samples that were positive for EVI1 or MDS1-EVI1. Linear correlation between Ct values and copy numbers was obtained for EVI1 and MDS1-EVI1, with correlation coefficients of 0.94 and 0.98, respectively. The efficiency of amplification, determined in cDNA obtained from a bone marrow sample from patient 2, was approximately 1.00 for EVI1, 0.95 for MDS1-EVI1, and 0.96 for PBGD. To evaluate whether the Ct method used in our study was indeed applicable, we
verified the differences in efficiencies of amplification in another 4 AML samples ( samples 25, 28, 29, and 30). The slopes of the fitted
lines for the mean Ct values at different mRNA concentrations were
0.089 for EVI1 and 0.036 for
MDS1-EVI1, indicating that the Ct method
was indeed applicable. The expression levels of MDS1
transcripts were too low to measure the efficiency of amplification.
Therefore we decided not to quantify the MDS1 expression
level but to show whether it was expressed (+) or not ().
As calibrator, we used the average expression of EVI1 and MDS1-EVI1 in 9 bone marrow samples from healthy volunteers. The mean Ct values of EVI1 and MDS1-EVI1 in these normal samples were 38.4 ± 1.5 and 38.3 ± 2.6, respectively. The values obtained were normalized for the internal reference, PBGD. The mean PBGD value for normal bone marrow samples was 23.2 ± 0.9. MDS1 expression was undetectable in bone marrow samples from healthy volunteers. EVI1, MDS1-EVI1, and MDS1 expression in AML patients carrying 3q26 abnormalities The relative expression of EVI1 and MDS1-EVI1 transcripts in patients with 3q26 abnormalities is shown in Table 3. In 3 AML patients carrying an inv(3)(q22;q26) and in 1 AML patient with a translocation t(3;3)(q22;q26), high expression of EVI1 as well as MDS1-EVI1 transcripts was observed. One of the 2 RAEB-t patients with t(3;12) showed high EVI1 levels and a moderate increase in MDS1-EVI1 expression, while in the other RAEB-t patient MDS1-EVI1 expression was high and EVI1 expression was comparable to that of normal bone marrow. A CML patient with t(3;17) showed weak expression of EVI1 and no detectable expression of MDS1-EVI1. Expression of MDS1 was observed in patient 6 only.Northern blot analysis was carried out using an EVI1 cDNA
probe on total mRNA isolated from 2 patients (patients 2 and 3) carrying inv(3)(q22q26) and a CML patient (patient 7) with a
translocation t(3;17) (Figure 2).
Although this probe does not discriminate between the EVI1
and MDS1-EVI1 transcripts, high expression as observed by real-time PCR was confirmed. No expression was observed by
Northern blot analysis in a normal bone marrow sample (N1) or in a
sample from an AML patient without 3q26 abnormality (C1); these 2 samples were negative for different transcripts as determined by
real-time PCR (Figure 2).
EVI1, MDS1-EVI1, and MDS1 expression in a cohort of 319 de novo AML patients The expression levels of EVI1, MDS1-EVI1, and MDS1 were next investigated by real-time PCR in bone marrow samples of 319 patients newly diagnosed with AML. This cohort did not include the 7 patients from Table 3. Of these 319 patients, 44 expressed EVI1, MDSI-EVI1, or both (Table 4): 6 expressed EVI1 only (group I), 26 expressed EVI1 as well as MDS1-EVI1 (group II), and 12 expressed MDS1-EVI1 only (group III). In the remaining 275 patients (group IV), neither EVI1 nor MDS1-EVI1 was expressed. In 15 patients the MDS1 gene expression (+) was detectable. MDS1 expression was always associated with high levels of EVI1 plus MDS1-EVI1 (Table 4).To confirm the results obtained by real-time PCR, Northern blot analysis was carried out in 6 cases. Patients who appeared to be highly positive for EVI1 and/or MDS1-EVI1 by real-time PCR (patients 8, 9, and 34) also showed the proper size transcripts by Northern blotting analysis (Figure 2). Two patients who were negative by real-time PCR (C2 and C3) and a normal bone marrow sample (N2) showed no transcripts by Northern blot analysis. 3q26 abnormalities in de novo AML samples expressing EVI1 or MDS1-EVI1 Cytogenetic analysis among the 44 patients who were positive for EVI1 and/or MDS1-EVI1 revealed that only 4 AML patients (patients 12, 23, 35, and 39) carried a 3q26 abnormality (Table 4). None of the patients within group IV (EVI1 and
MDS1-EVI1) carried 3q26 aberrations.
EVI1 expression correlates with unfavorable karyotypes 3q26 defects in AML are frequently accompanied by additional unfavorable cytogenetic abnormalities (eg,7/7q). In fact, in 2 cases shown in Table 3 (patients 1 and 6) and all 4 cases with a 3q26
aberration shown in Table 4 (patients 12, 23, 35, and 39), chromosome 7 abnormalities were observed. We next investigated whether
EVI1 and/or MDS1-EVI1 expression in de
novo AML without a 3q26 abnormality also correlated with the presence
of poor-risk karyotypes (Tables 4 and 5). In 67% of patients (4 of 6)
from group I (EVI1 only) and 42% of patients (11 of 26)
from group II (EVI1+ and
MDS1-EVI1+), unfavorable karyotypic
abnormalities (ie, 7/7q, 5/5q, t(9;22), t(6;9)) or complex
karyotypes (> 3 abnormalities) were present. In contrast, only 8% (1 of 12) of the patients from group III (EVI1
and MDS1-EVI1+) and 12% (33 of 275)
of the patients from group IV (EVI1 and
MDS1-EVI1) carried unfavorable
karyotypes. Thus EVI1 expression (groups I and II)
correlated with unfavorable karyotypes (P < .0001), whereas the presence of MDS1-EVI1 alone (group
III) did not. Moreover, favorable-risk karyotypes, that is, t(8;21),
t(15;17) or inv(16), were not noted in any of the
EVI1-expressing AML patients from group I or group II. In
contrast, 33% (4 of 12) of the patients in group III
(EVI1 and
MDS1-EVI1+) and 19% (53 of 275) of
the patients in group IV (EVI1 and
MDS1-EVI1) exhibited favorable-risk
karyotypes (Table 5).
Among the 212 cases that belong to the leukemias with intermediate-risk karyotypes, 14 showed an 11q23 translocation (MLL rearrangement). Interestingly, 8 of these 14 patients (57%) were found in group II, that is, patients expressing EVI1+ and MDS1-EVI1+ (Table 4). These data suggest a strong correlation between EVI1 expression and the presence of MLL rearrangements. Correlation of EVI1 and MDS1/EVI1 expression with other prognostic indicators We next investigated whether EVI1 and/or MDS1/EVI1 expression showed any correlation with other known prognostic indicators. Internal tandem duplication in the FLT3 receptor tyrosine kinase gene has been observed in approximately 20% to 30% of patients with AML. Moreover, this mutation appears to confer a poor prognosis in many studies carried out in the past 5 years.25,26 In 85 (27%) of the 319 cases investigated, an FLT3-ITD was found. As shown in Table 5, high EVI1 and MDS1-EVI1 expression rarely coincided with FLT3-ITD mutation. Almost all the patients with an FLT3-ITD mutation belonged to group IV, and 82% (70 of 85) carried an intermediate-risk karyotype. No significant correlation was found between EVI1 and/or MDS1-EVI1 expression and sex, age, WBC count, platelet count, blast counts in blood or bone marrow, or FAB classification (data not shown).EVI1 an independent unfavorable prognostic marker in the intermediate-risk karyotypic group All patients received induction therapy and were included in the survival analysis. Clinical outcome was investigated in the distinct groups of patients based on their EVI1 and MDS1-EVI1 expression. Survival analysis was performed using a cutoff value of 50. The remission rates for patients in groups I, II, III, and IV were 50%, 77%, 67%, and 79%, respectively (Table 6). All patients in group I died within 12 months, and 25 of 26 patients in group II (EVI1+ and MDS1-EVI1+) died within 30 months (Table 6; Figure 3A,B).
The actuarial survival probabilities at 60 months were 33% in groups III and IV and only 0% for group I and 8% for group II (Table 6; Figure 3A,B). Thus patients in groups I and II had a significantly shorter OS (P = .002). The EFS probabilities at 60 months for groups I and II (0% and 4%, respectively) were much lower than those for groups III and IV (25% and 27%, respectively). A significant difference (P = .002) was also observed between EFS in patients with high EVI1 expression (groups I and II) and that in AML patients without EVI1 expression (groups III and IV). We also analyzed survival using alternative cutoff values (ie, 10, 25, and 100). Although the numbers of patients within the 4 different subgroups were slightly different, changing the cutoff values did not alter the conclusions drawn from the analysis at cutoff value 50 (data not shown). Cox regression analysis was applied to assess the prognostic
significance of high EVI1 expression for OS and EFS (Table
7). High EVI1 expression was
associated with an increased hazard ratio for death (OS; HR = 1.85)
or failure (EFS; no CR, death in CR, or relapse, HR = 1.82), which
was statistically significant in univariable analysis. After adjustment
for karyotypic risk factors, age, and FLT3 in a multivariable analysis,
high EVI1 expression was still associated with an increased
hazard ratio (P = .09). As high EVI1 expression
is often associated with unfavorable karyotypes, its prognostic value
seemed to be overshadowed. To exclude the effect of unfavorable
cytogenetics, we decided to investigate the prognostic value of
EVI1 in the intermediate-risk group.
We investigated whether high EVI1 expression would be of
prognostic value for patients carrying intermediate-risk karyotypes, as
half of the patients with EVI1 expression (groups I and II) belonged to this risk group. As shown in Figure
4A-B, intermediate-risk patients with
high EVI1 expression (n = 16) had significantly shorter OS
and EFS (P = .05 and P = .03) than their
EVI1-negative counterparts (n = 196). Furthermore, the
disease-free survival was significantly shorter in patients
with high EVI1 expression (P = .007). None of
the EVI1-overexpressing patients carried an FLT3-ITD, whereas FLT3-ITD was observed in 36% (70 of 196) of the patients without high EVI1 expression. Univariable and
multivariable analysis revealed that high EVI1 expression
serves as an independent prognostic marker for EFS and OS in the
intermediate-risk group (Table 8).
We developed a sensitive method to quantify EVI1 and its fusion transcript MDS1-EVI1 in AML. Our data demonstrate that EVI1 rather than MDS1-EVI1 is a strong indicator for poor treatment response and survival. MDS1 expression was seen in 5% (15 of 319) of the AML patients and was always associated with high EVI1 and MDS1-EVI1 expression. High EVI1 mRNA expression was observed in 10% (32 of 319) of the patients with newly diagnosed AML. Poor clinical outcome of patients with 3q26 abnormality has previously been reported.3,27-29 As we demonstrated in this study, patients with 3q26 abnormality represent a minor subgroup of patients with high EVI1 expression. In fact, only 12.5% (4 of 32) of the patients with high EVI1 expression carried a 3q26 abnormality. High EVI1 expression was significantly correlated with the presence of unfavorable cytogenetic abnormalities. Favorable-risk karyotypes were not present among the EVI1-expressing groups. Evi1 was first discovered as a proto-oncogene in retrovirally induced myeloid leukemias in the mouse. Retroviral insertions in the Evi1 locus in those tumors mostly occurred in close vicinity of the first 2 or 3 exons of Evi1, causing Evi1 overexpression.30 These data underline that EVI1 rather than MDS1-EVI1 is the transforming gene in AML. This conclusion is further strengthened by the fact that in the cohort of de novo AML patients investigated in the present study, EVI1 and not MDS1-EVI1 expression correlated with unfavorable-risk leukemias. In contrast to what has been published previously, we demonstrate that EVI1 expression in de novo AML may be an important parameter to define a subgroup of poor-risk AML patients. Langabeer et al31 studied 197 de novo AML patients but did not find any prognostic value for high EVI1 expression. This is not surprising, however, as the investigators did not discriminate between EVI1 and MDS1-EVI1 expression. A number of EVI1-positive patients in this study carried a favorable-risk karyotype, indicating that these patients are most likely expressing MDS1-EVI1 rather than EVI1. Furthermore, previous studies were carried out using classical reverse transcriptase-PCR, while we performed quantitative real-time PCR to be able to determine high EVI1 expression levels based on a defined cutoff value. Cytogenetic analysis provides a powerful approach to discriminate
between favorable-risk and unfavorable-risk groups of AML patients.
However, using karyotyping, only 30% to 40% of the AML patients can
be classified within these 2 subgroups. In other words, a majority of
AML patients belong to intermediate or unknown karyotypic risk groups.
A major challenge will be discriminating favorable-risk from
unfavorable-risk patients within this heterogeneous group of patients
by means of molecular biologic approaches. Among the patients
with intermediate-risk karyotype studied, 33% (70 of 212) harbored an
FLT3-ITD mutation that predicts poor prognosis. Sixteen (8%) of the
212 patients had high EVI1 expression and showed very poor
survival. Interestingly, none of the EVI1-positive patients
harbored an FLT3-ITD, indicating that the EVI1-expressing group represents a distinct subclass of poor-response leukemias. Within
the same group of patients with intermediate-risk karyotype, a
subpopulation of poor responders has been defined with very low mRNA
levels of the CEBP In 8 of 14 patients with an 11q23 abnormality, we observed high EVI1 and MDS1-EVI1 expression. In previous studies, the correlation between EVI1 and 11q23 has been overlooked, as the cohorts were not large enough and the methods used did not discriminate between EVI1 and MDS1-EVI1. Two of 16 EVI1-positive patients screened by Ohyashiki et al17 and 1 of 29 EVI1-positive patients studied by Langabeer et al31 carried an 11q23 abnormality. Translocations involving chromosome band 11q23 disrupt the MLL gene. MLL is a putative transcription regulator that may form complexes with other transcription factors. It contains both a strong activation domain and a repression domain.33 In de novo leukemia, 75% of the breakpoints in MLL are mapped to the centromeric half of the breakpoint cluster region (BCR),34 which is located in the repression domain. Disruption of the repression domain in these particular 11q23 translocations might lead to an alteration of the repressor function of MLL, leading to an up-regulation of downstream target genes. A possible explanation for high EVI1 and MDS1-EVI1 expression in a large proportion of patients with 11q23 defects could therefore be that transcription of those genes is under the control of MLL. We hypothesize that MLL normally represses EVI1 and MDS1-EVI1 expression. This repression might then be disrupted as a result of a chimeric protein generated by 11q23 translocation, as has been suggested for Hox genes.5 Cloning and nucleotide sequencing analysis of the MLL fusion genes in EVI1-positive versus EVI1-negative patients may provide critical information on this issue. One of the goals of this study was to investigate whether EVI1, MDS1-EVI1, or both transcripts were expressed in AML patients with 3q26 aberrations. All of the 8 AML patients with a classical t(3;3) or an inv(3) (Tables 3 and 4) showed high expression of EVI1. High EVI1 expression in 7 patients was associated with high MDS1-EVI1. Thus, although our data suggest that EVI1 rather than MDS1-EVI1 is the critical gene involved in transformation of myeloid precursors, it is noteworthy that MDS1-EVI1 is also frequently expressed. Since the 3q26 breakpoints are often located between the MDS1 and EVI1 loci, the normal allele is responsible for MDS1-EVI1 expression. MDS1-EVI1 expression might be directly or indirectly up-regulated by EVI1 expression. Another possible explanation is that aberrantly expressed EVI1 as a result of 3q26 aberration mainly transforms progenitor cells that normally have high EVI1 and/or MDS1-EVI1 levels. Fractionated CD34+ progenitor cell populations indeed show high EVI1, MDS1-EVI1, and MDS1 expression (S.B.v.W.v.D.-K. et al, unpublished data, January 2001). Previous studies10,35 also confirmed EVI1 expression in early CD34+ progenitor cells. In fact, transformation in these progenitors may be a result of a disturbance in the tightly controlled balance between EVI1 and its fusion transcript. The mechanism by which EVI1 is expressed in patients without 3q26 is unknown. It is conceivable that defects in the EVI1 promoter or in EVI1-regulatory genes affect expression. It is also possible that CD34+ progenitor cells naturally expressing EVI1 and/or MDS1-EVI1 are transformed by other mechanisms and arrested at that particular stage of differentiation. It should be noted that high EVI1 expression is often associated with the presence of poor-risk abnormalities. For example, complex karyotypes are seen twice as often in patients with high EVI1 expression than in patients without EVI1 expression. Our data point to a critical role that EVI1 may play in genomic instability in AML patients expressing this gene. The data presented here demonstrate that EVI1 overexpression in AML patients correlates with poor treatment outcome. We propose that EVI1 gene expression measurements with real-time PCR should be incorporated into the diagnostic procedures for de novo AML patients, especially in the subpopulation with intermediate- or unknown-risk karyotypes. Determination of EVI1 expression levels in this particular category of AML patients will be useful in distinguishing a subgroup of patients with poor prognosis. Identification and classification of subtypes of AML with specific molecular defects will be of great value in designing unique stratified treatment approaches.
A. Hagemeijer of the Center for Human Genetics, Catholic University of Leuven, Belgium, is acknowledged for the cytogenetic analysis performed on patient samples obtained from that institute. We thank our colleagues from the bone marrow transplantation group and the molecular diagnostics laboratory, Institute of Hematology, Erasmus Medical Centre, for preparation and storage of AML samples. We wish to thank Karola van Rooijen for preparation of the figures.
Submitted May 20, 2002; accepted September 6, 2002.
Prepublished online as Blood First Edition Paper, October 3, 2002; DOI 10.1182/blood-2002-05-1459.
Supported by grants from the Dutch Cancer Society (Koningin Wilhelmina Fonds) and the Erasmus University Medical Centre (Revolving Fund).
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734.
Reprints: Ruud Delwel, Institute of Hematology, Erasmus Medical Centre, PO Box 1738, 3000 DR Rotterdam, Netherlands; e-mail: delwel{at}hema.fgg.eur.nl.
1.
Jolkowska J, Witt M.
The EVI-1 gene
2.
Morishita K, Parganas E, William CL, et al.
Activation of EVI1 gene expression in human acute myelogenous leukemias by translocations spanning 300-400 kilobases on chromosome band 3q26.
Proc Natl Acad Sci U S A.
1992;89:3937-3941 3. Reiter E, Greinix H, Rabitsch W, et al. Low curative potential of bone marrow transplantation for highly aggressive acute myelogenous leukemia with inversion inv (3)(q21q26) or homologous translocation t(3;3) (q21;q26). Ann Hematol. 2000;79:374-377[CrossRef][Medline] [Order article via Infotrieve]. 4. Nucifora G. The EVI1 gene in myeloid leukemia. Leukemia. 1997;11:2022-2031[CrossRef][Medline] [Order article via Infotrieve]. 5. Kawagoe H, Kawagoe R, Sano K. Targeted down-regulation of MLL-AF9 with antisense oligodeoxyribonucleotide reduces the expression of the HOXA7 and -A10 genes and induces apoptosis in a human leukemia cell line, THP-1. Leukemia. 2001;15:1743-1749[Medline] [Order article via Infotrieve]. 6. Russell M, Thompson F, Spier C, Taetle R. Expression of the EVI1 gene in chronic myelogenous leukemia in blast crisis. Leukemia. 1993;7:1654-1657[Medline] [Order article via Infotrieve]. 7. Xi ZF, Russell M, Woodward S, Thompson F, Wagner L, Taetle R. Expression of the Zn finger gene, EVI-1, in acute promyelocytic leukemia. Leukemia. 1997;11:212-220[CrossRef][Medline] [Order article via Infotrieve].
8.
Russell M, List A, Greenberg P, et al.
Expression of EVI1 in myelodysplastic syndromes and other hematologic malignancies without 3q26 translocations.
Blood.
1994;84:1243-1248 9. Dreyfus F, Bouscary D, Melle J, Ribrag V, Guesnu M, Varet B. Expression of the Evi-1 gene in myelodysplastic syndromes. Leukemia. 1995;9:203-205[Medline] [Order article via Infotrieve]. 10. Privitera E, Longoni D, Brambillasca F, Biondi A. EVI-1 gene expression in myeloid clonogenic cells from juvenile myelomonocytic leukemia (JMML). Leukemia. 1997;11:2045-2048[CrossRef][Medline] [Order article via Infotrieve]. 11. Morishita K, Parganas E, Parham DM, Matsugi T, Ihle JN. The Evi-1 zinc finger myeloid transforming gene is normally expressed in the kidney and in developing oocytes. Oncogene. 1990;5:1419-1423[Medline] [Order article via Infotrieve].
12.
Kreider BL, Orkin SH, Ihle JN.
Loss of erythropoietin responsiveness in erythroid progenitors due to expression of the Evi-1 myeloid-transforming gene.
Proc Natl Acad Sci U S A.
1993;90:6454-6458 13. Fears S, Mathieu C, Zeleznik-Le N, Huang S, Rowley JD, Nucifora G. Intergenic splicing of MDS1 and EVI1 occurs in normal tissues as well as in myeloid leukemia and produces a new member of the PR domain family. Proc Natl Acad Sci U S A. 1996;93:1642-1647 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Top
Abstract
Introduction
gene.
its role in pathogenesis of human leukemias.
Leuk Res.
2000;24:553-558