Blood, Vol. 101, Issue 3, 921-928, February 1, 2003
Supplemental materials for: Norman et al, Vol 101, Issue 3, 921-928.
Supplemental Videos:
Video 1. A typical cremaster venule 4 hours 0 minutes after stimulation with TNF-
(MOV file, 3.2 MB): A number of leukocytes are engaged in interaction (rolling and firm adhesion) with the endothelium, although the level of this interaction is typically less than that seen 2 to 3 hours after TNF-. Application of RB6-8C5 at 4 hours causes a rapid fall in blood flow, which comes to an almost complete standstill by 4 hours 3 minutes. RBCs continue to pack into the vessel until it finally takes on a glassy clotted appearance at 4 hours 5 minutes. Clearance of this vessel appears to begin at 4 hours 7 minutes, but it reclots by 4 hours 9 minutes. This mouse died at 4 hours 13 minutes. This video runs at 10 times normal speed and is best viewed with the video window maximized.
Video 2. A leukocyte-rich aggregate attached to a venule (comes into clear focus at 4 hours 15 minutes 15 seconds) (MOV file, 1.4 MB): This aggregate detaches at 4 hours 15 minutes 24 seconds and is washed downstream. TNF- was injected at 0 hours and RB6-8C5 at 4 hours 14 minutes. This video runs at normal speed and is best viewed with the video window maximized.
Video 3. Fluorescent albumin in 3 venules of a mouse cremaster about 4 hours after injection of TNF- (MOV file, 4.5 MB): FITC-albumin (50 
L) was injected intravenously at 4 hours 15 minutes and remained vessel bound until 4 hours 21 minutes when RB6-8C5 was injected. A gradual darkening of the vessel image and brightening of the surrounding tissues indicates marked plasma leakage. This video runs at 20 times normal speed and is best viewed with the video window maximized.
Video 4. A venule (left) and arteriole (right) in a mouse cremaster muscle 4 hours 17 minutes after injection of TNF- (MOV
file, 1.8 MB): RB6-8C5 was injected intravenously at 4 hours 17 minutes.
Rolling leukocytes can be seen in both the arteriole and venule, although at this time after TNF- there is
typically more arteriolar rolling. Emboli can be seen passing through the venule at 4 hours 17 minutes 30 seconds and through the arteriole at 4 hours 17 minutes 54 seconds. As blood flow falls, the hematocrit in the arteriole lowers until only plasma is left. This probably reflects an upstream obstruction, which might be a lodged embolus, preventing entry of cells to the vessel. This video runs at 10 times normal speed and is best viewed with the video window maximized.
Video 5. Leukocytes rolling in a venule 3 hours after
TNF- injection (MOV file, 1.7 MB). The 3-hour time point was chosen for this video because there is typically more rolling 3 hours after TNF- than at 4 hours. The sequence begins at 3 hours 1 minute 30 seconds and RB6-8C5 was injected at 3 hours 2 minutes 50 seconds. Rolling leukocytes arrest at 3 hours 3 minutes 15 seconds indicating that the effects of RB6-8C5 are transduced extremely rapidly. This video runs at 10 times normal speed and is best viewed with the video window maximized.
