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Blood, Vol. 101, Issue 3, 929-936, February 1, 2003
Supplemental materials for: Patel et al, Vol 101, Issue 3, 929-936.
Supplemental Videos:
Video A1. Adhesion of untreated, mouse IgG-treated and monoclonal antibody 7E3-treated human platelets to a collagen-coated surface (MOV file, 5 MB)
With untreated platelets and mouse IgG–treated platelets, initially a small number of platelets ("vanguard" platelets) adhere directly to the collagen. Then, while additional vanguard platelets adhere directly to the collagen, other platelets in suspension ("follower" platelets) become tethered to vanguard platelets via filopodia. Follower platelets remain tethered to vanguard platelets for variable periods of time, after which they adhere to nearby collagen directly and spread, or begin to spread over the vanguard platelet to which they initially attached. This results in the development of a sheetlike underlayer of platelets and the growth of platelet thrombi as islands. Ultimately, platelets spreading from different islands contact each other, and there appears to be movement of the contents of the platelets towards each other. Active ruffling of the edges of the interacting platelets continues thereafter.
In the presence of monoclonal antibody 7E3, platelets adhere to collagen and extend multiple filopodia that show dynamic movements, but spread more variably and less extensively than in the absence of 7E3. In marked contrast to the results in the absence of 7E3, there is virtually no tethering of follower platelets, platelet thrombi, or evidence of platelets acting as an interacting mass. In addition, the platelets develop a more uniformly scattered pattern, and the borders of the platelets remain distinct. This video runs at 5 frames per second. 15 seconds of running video time corresponds to about 23 minutes of elapsed time of adhesion. The distance between the 2 white dots in the upper right is 10  m.
Video A2. Adhesion of human platelets to collagen in the presence of mouse IgG, 7E3, or tirofiban (MOV file, 5 MB)
The results with mouse IgG and 7E3 are similar to those using the same treatments observed in Video A1. The results with tirofiban are similar to those observed with antibody 7E3. The video runs at 5 frames per second. 15 seconds of running video time corresponds to 90 minutes of elapsed time of adhesion.
Video B. Adhesion of wild-type mouse platelets,  3-null mouse platelets, and wild-type mouse platelets in the presence of antibody 1B5 (anti- IIb3) to collagen (MOV file, 5 MB)
Wild-type mouse platelets adhere to collagen much like human platelets (see Video A1-2) although loss of the granulomere structures is variable and less pronounced. 3-null mouse platelets and wild-type mouse platelets in the presence of 1B5 adhere to collagen in a manner similar to that of human platelets in the presence of antibody 7E3 or tirofiban (see Video A1-2). The video runs at 5 frames per second. 15 seconds of running video time corresponds to 25 minutes of elapsed time of adhesion.
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