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Prepublished online as a Blood First Edition Paper on October 31, 2002; DOI 10.1182/blood-2002-05-1461.
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
From the Departments of Surgery and Molecular Genetics
and Biochemistry, School of Medicine, University of Pittsburgh Cancer
Institute, University of Pittsburgh, PA; Department of Geriatric and
Respiratory Medicine, Tohoku University School of Medicine, Sendai,
Japan; Department of Medical Biochemistry, Graduate School
of Medical Sciences, Kyushu University, Fukuoka, Japan;
Department of Immunology, Schering-Plough Research Institute,
Kenilworth, NJ; Cell Resource Center for Biomedical Research, Institute
of Development, Aging and Cancer, Tohoku University, Sendai,
Japan.
Interleukin-17 (IL-17) is a CD4 T-cell-derived proinflammatory
cytokine. We investigated the effects of locally produced IL-17 by
tumors as a means to evaluate its biologic function. Although recombinant IL-17 protein or retroviral transduction of
IL-17 gene into tumors did not affect in vitro
proliferation, IL-17 transfectants grew more rapidly in vivo when
compared with controls. Immunostaining for Factor VIII revealed that
tumors transduced with IL-17 had significantly higher vascular density
when compared with controls. IL-17 indeed elicited neovascularization
in rat cornea. In addition, angiogenic activity present in the
conditioned media of CD4 T cells was markedly suppressed by
neutralizing monoclonal antibody to IL-17. IL-17 had no
direct effect on the growth of vascular endothelial cells, whereas
IL-17 significantly stimulated migration. IL-17 also markedly promoted
the cord formation of vascular endothelial cells. In addition, IL-17
up-regulated elaboration of a variety of proangiogenic factors by
fibroblasts as well as tumor cells. These findings reveal a novel role
for IL-17 as a CD4 T-cell-derived mediator of angiogenesis that
stimulates vascular endothelial cell migration and cord formation and
regulates production of a variety of proangiogenic factors.
Furthermore, they suggest that inhibition of biologic action of IL-17
may have therapeutic benefits when applied to angiogenesis-related disorders.
(Blood. 2003;101:2620-2627) Angiogenesis, the formation of new capillaries from
pre-existing blood vessels, is a fundamental process involved in
physiologic and pathologic conditions, including wound healing,
rheumatoid arthritis, psoriasis, and tumorigenesis.1 The
physiologic regulation of angiogenesis is dependent on the proper
temporal and spatial delicate balance of activators and inhibitors
present within the vascular microenvironment.2 Tumor
growth is an angiogenesis-dependent process that requires the sustained
new vessel growth.3 Tumors or interacting stromal
cells may cause imbalance to increase elaboration of angiogenic
inducers or decrease production or effects of angiogenic suppressors.
Identification of endogenous angiogenesis stimulators or inhibitors is
an area of great interest.3
Interleukin-17 (IL-17) was originally cloned by subtractive
hybridization from an activated T-cell library and initially termed cytotoxic T-lymphocyte-associated antigen (CTLA)-8.4
Recently, several homologous proteins with similar or different
biologic profiles have been identified.5 Human IL-17 is
produced predominantly by activated CD4 T cells and has sequence
homology with the open reading frame 13 of the T-lymphotropic
herpesvirus saimiri.6 IL-17 has several biologic
activities, including induction of IL-6, IL-8, and prostaglandin
E2 (PGE2), as well as enhancement of
intercellular adhesion molecule (ICAM)-1 expression in fibroblasts and
keratinocytes.6-9 In addition, IL-17 induces secretion of tumor necrosis factor (TNF)- There are multiple means to evaluate biologic functions of cytokines
and chemokines. One way to define the biologic actions of a cytokine is
to express it under a strong constitutive or inducible promoter in cell
lines such as tumors or in mice and observe its local and systemic
effects. We constructed a retroviral vector-expressing mouse IL-17 and
the NeoR selectable marker (DFG-mIL-17-IRES-Neo) and transduced tumor
cells to evaluate biologic effects in situ. IL-17 secreted by
transfectants markedly enhanced in vivo tumor growth and significantly
increased tumor vascularity. These results suggest that IL-17 produced
by tumors may promote in vivo growth via potentiation of tumor
angiogenesis. Actually, IL-17 elicited neovascularization in rat cornea
assay. Our findings indicate that CD4 T-cell-derived cytokine IL-17 is
a novel member of the angiogenic factor family and has a prominent role
in angiogenesis-related situations.
Mice and reagents
Cells and cell cultures
Construction of recombinant retroviral vector carrying mouse IL-17 A retroviral vector DFG-mIL-17-IRES-Neo was constructed as follows. MFG-mIL-17 was constructed using fragments of Nco1-BsmA1 adaptor, BsmA1-EcoR1 fragment of mouse IL-17 cDNA, EcoR1-BamH1 fragment of the polymerase chain reaction products of IL-17 cDNA, and a retroviral vector termed MFG.13 IRES-Neo cassette, consisting of an EMCV IRES sequence (Novagen, Madison, WI) and cDNA of Neo obtained from pMC1neo poly(A) (Stratagene, La Jolla, CA), was subcloned into the BamH1 site of MFG-mIL-17. Transfecting DFG-mIL-17-IRES-Neo proviral construct into ecotropic packaging cell line BOSC2314 (provided by Drs Pear and Baltimore, Rockefeller University, New York, NY) generated retroviral supernatant. The titer of the retroviral supernatant used in the subsequent experiments was 1.0 to 1.2 × 106 CFU/mL. Target cells were infected with 2 mL DFG-mIL-17-IRES-Neo retroviral supernatants in the presence of polybrene (8 µg/mL) and selected in CM with 800 µg/mL G418. As a control, a retroviral vector carrying only neomycin phosphotransferase gene (G1Na, provided by GTI, Germantown, MD) was used.Flow cytometric analysis Surface expression of MHC class I (H-2Kb), CD56, and CD44 antigens on tumor cells was examined by a fluorescence-activated cell sorter scan (Becton Dickinson, San Jose, CA) by using the monoclonal antibodies conjugated with fluorescein isothiocyanate (FITC) or phycoerythrin (PE) purchased from PharMingen (San Diego, CA).In vitro cell growth assay To evaluate the effect of IL-17 on the proliferation of vascular endothelial cells, HUVECs or HMVECs were suspended in EBM with 50 µg/mL gentamycin, 50 ng/mL amphotericin-B, and 2% FCS and were plated at 1.5 × 105 cells in 10-cm culture dish coated with collagen (Becton Dickinson, Bedford, MA). After 24 hours (on day 0), medium was then replaced with EBM containing 50 µg/mL gentamycin, 50 ng/mL amphotericin-B, and 2% FCS with or without cytokines. The cell number was counted on day 5.3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyltetrazolium bromide (MTT) assay Cells (1 × 103) were seeded into 96-well flat-bottomed plates and cultured in RPMI 1640 containing 3.5% FCS with or without 0.5 to 500 ng/mL IL-17. On day 5, cells were washed with medium, and 100 µL MTT (Sigma) solution (2.5 mg/mL in RPMI 1640 with 10% FCS) was added to each well. Plates were incubated for 90 minutes. Next, MTT solution was removed and 50 µL dimethylsulfoxide (DMSO) (Sigma) was added to each well to solubilize formazan crystals formed in viable cells. The absorbance was read at a wavelength of 590 nm on enzyme-linked immunosorbent assay (ELISA) plate reader.In vivo evaluation of tumor growth Mice were inoculated subcutaneously in the right flank with 1 × 105 MCA205WT, MCA205Neo, or MCA205IL-17 or with 2 × 105 MC38WT, MC38Neo, or MC38IL-17. In some experiments, mice received 500 rad of whole-body irradiation. After 24 hours, tumor cells were inoculated. Tumor volume (cubic millimeter) was calculated by using the formula ab2/2 (a = largest diameter; b = smallest diameter).15Histologic examination and quantitation of vessel density Tumor tissues were harvested, fixed in 10% buffered formalin, processed, embedded in paraffin, and sectioned with a microtome. Sections were stained with hematoxylin and eosin or stained for Factor VIII.For Factor VIII staining, sections were deparaffinized in xylene, dehydrated in graded concentrations of ethanol, and then rinsed in distilled water. Sections were digested with protease XXIV (Sigma), and then washed with distilled water. Endogenous peroxidase activity was blocked by 3% aqueous hydrogen peroxide, and nonspecific proteins were blocked by using protein blocker (Shanndon Lipshaw, Pittsburgh, PA). Then, rabbit antihuman factor VIII F(ab')2 (DAKO, Carpinteria, CA) was applied. After washing, the sections were incubated with biotinylated goat antirabbit F(ab')2 antibody (Jackson ImmunoResearch, West Grove, PA). After washing, avidin biotin complex solution (Vector, Burlingame, CA) was applied and then developed with 3-amino-9-ethylcarbazole (ScyTek, Logan, UT). Finally, the sections were weakly counterstained with hematoxylin. Tumor sections were examined in a blinded fashion for the presence of Factor VIII immunolocalization. Factor VIII staining was evaluated by quantifying the number of stained blood vessels in 5 randomly chosen high-power fields (HPFs) (× 200) per tumor. A total of 25 HPFs were examined and counted from 5 tumors of each of the groups. Any distinct area of positive staining for Factor VIII was counted as a single, regardless of size. The results were shown as mean ± SD per square millimeter considering the area of HPF. Rat cornea assay The cornea assay was performed as described previously.16 Briefly, hydron pellets (Interferon Sciences, New Brunswick, NJ) containing phosphate-buffered saline (PBS) alone, 250 or 500 ng mouse IL-17, or 100 ng human bFGF were implanted into the cornea of anesthetized rats. In some experiments, hydron pellets containing 250 ng mouse IL-17 and 1 µg control antibody or antimouse IL-17 mAb were implanted into the cornea. After 7 days, the animals were killed, and cornea vessels were photographed. Eight rats were used to assess the effect on neovascularization for each group. The experiment was repeated twice.Matrigel plug assay Mice were injected subcutaneously at the abdominal midline with 0.5 mL Growth Factor-Reduced Matrigel Matrix (Becton Dickinson) in the presence of 8 U/mL heparin (Sigma) supplemented with conditioned media of CD4 T cells and 20 µg/mL control Ab or neutralizing antihuman IL-17 mAb. After 6 days, Matrigel plugs were removed, fixed with 4% buffered paraformaldehyde, and embedded in paraffin. Sections were stained with hematoxylin and eosin. Vessel area and the total Matrigel area were planimetrically assessed from 3 stained sections per Matrigel plug with the use of National Institutes of Health imaging. The experiment was repeated twice with 5 mice per group in each experiment.Migration assay Migration of HMVECs or HUVECs was evaluated by using a modified Boyden chamber assay, as described previously.17 Cells were cultured in EBM with 2% FCS for 8 hours and plated at 12 × 104 cells/cm2 onto the polycarbonate filter with 5-µm pores (KURABO) coated with 10 µg/mL fibronectin (Sigma). Cytokines in EBM with 1% FCS were applied in the lower compartments of the chamber, and cells suspended in EBM with 1% FCS were cultured for 4 hours at 37°C. The filters were fixed and stained with Diff-Quick (Harleco, Gibbstown, NJ), and the number of migrating cells was quantified by counting cells in 5 randomly selected microscopic fields (× 200) in each well. For inhibitory assay, neutralizing antihuman IL-17 mAb (20 µg/mL) was added in the lower and upper compartments of the chamber.Endothelial cord formation In vitro cord formation was performed according to the manufacturer's instruction (KURABO).18 Briefly, HUVECs and human dermal fibroblasts were admixed and seeded into 24-well plates in medium with or without cytokine. Cells were incubated for up to 11 days, and the medium was replaced with fresh every 3 days. HUVECs were stained for Factor VIII. The analyses were made by computerized image analysis of the number of pixels occupied by the tubules in a total of 15 random areas from these separate wells.Cytokine and nitric oxide (NO) assays Fibroblasts or tumor cells (1 × 105/mL) were cultured in RPMI 1640 containing 3.5% FCS with or without IL-17 for 48 hours. Cell-free supernatants were collected and stored at 70°C.
Concentrations of angiogenic factors were measured by ELISA kits (R&D
Systems). To assess the production of NO, the concentration of nitrite
was measured with the use of the Griess reaction, as described
previously.19
Reverse transcription-polymerase chain reaction (RT-PCR) The primer sequences of the oligonucleotides used for PCR were as follows: -actin, sense: 5'-TTCTACAATGAGCTGCGTGTG-3',
antisense: 5'-CACTGTGTTGGCATAGAGGTC-3'; IL-17, sense:
5'-ATGAGTCCAGGGAGAGCTTCAT-3', antisense: 5'-TTAGGCTGC CTGGCGGACAATC-3';
acidic fibroblast growth factor (aFGF), sense: 5'-GCAGCCC
TGACCGAGAGGTTC-3', antisense: 5'-CTTACAGCTCCCGTTCTTCTT-3'; bFGF, sense:
5'-ACACGTCAAACTACAACTCCA-3', antisense: 5'-TCAGCTCTTAGCAGAC ATTGG-3';
transforming growth factor (TGF)-, sense: 5'-CTAATGGTGGACCGCAA
CAAC-3', antisense: 5'-CGGTTCATGTCATGGATGGTG-3'. Total RNA was
extracted by using RNAzolTMB (TEL-TEST, Friendswood, TX) according to
manufacturer's instruction. Total RNA (4 µg) was applied for the
synthesis of cDNA with SuperScriptTM RNaseH-Reverse Transcriptase (Life
Technologies). PCR was performed in a DNA Thermal Cycler (Perkin-Elmer
Cetus, Norwalk, CT) using Taq polymerase (Boehringer
Mannheim, Indianapolis, IN).
Statistical analysis Statistical analysis was performed using an unpaired 2-tailed Student t test with a confirmation by parametric and F tests. Difference was considered significant with a P value less than .05.
Establishment and characterization of IL-17-producing tumor cell lines We generated a retroviral vector DFG-mIL-17-IRES-Neo as described in "Materials and methods" and used it to transduce the fibrosarcoma MCA205 as well as the colon adenocarcinoma MC38. No expression of either IL-17 mRNA or protein could be detected in MCA205 and MC38 before transfection (data not shown). After G418 selection, expression of IL-17 mRNA by stable transfectants was determined by RT-PCR (data not shown). MCA205IL-17 or MC38IL-17 secretes 153 or 43 ng/1 × 106 cells per 48 hours, respectively, determined by ELISA. The biologic activity of expressed protein in the supernatants of MCA205IL-17 or MC38IL-17 was confirmed by its ability to induce IL-6 production (Table 1). Flow cytometric analysis demonstrated that the surface ICAM-1 expression of IL-17 transfectants was slightly enhanced when compared with parental cells (data not shown), which was consistent with a previous report.6 The expression of H-2Kb and CD44 was not altered (data not shown).
IL-17 significantly promotes in vivo tumor growth IL-17 has no direct effect on proliferation of either MCA205 or MC38 cells determined by MTT assay (Figure 1A). Significant changes were not observed in in vitro growth of IL-17 transfectants when compared with that of parental cells or neomycin phosphotransferase gene transfectants (Figure 1A). When MCA205WT, MCA205Neo, or MCA205IL-17 cells were implanted subcutaneously into syngeneic mice, they all formed solid tumors. However, MCA205IL-17 developed tumors with an increased growth rate compared with MCA205WT or MCA205Neo (MCA205IL-17 versus MCA205WT, P < .008; MCA205IL-17 versus MCA205Neo, P < .0004, on day 25) (Figure 1B). A similar increase in in vivo tumor growth was observed when MC38IL-17 was implanted subcutaneously into the syngeneic animals compared with MC38WT or MC38Neo (MC38IL-17 versus MC38WT, P < .0002; MC38IL-17 versus MC38Neo, P < .0002, on day 30) (Figure 1C).
We next examined whether immunologic mechanisms were involved in the increased in vivo growth of IL-17 transfectants. To address this issue, groups of mice were irradiated and challenged with MCA205Neo or MCA205IL-17 cells. Even in irradiated mice, MCA205IL-17 grew more rapidly when compared with control (MCA205IL-17 versus MCA205Neo, P < .002 [normal mice]; MCA205IL-17 versus MCA205Neo, P < .01 [irradiated mice] on day 28) (Figure 1D). Therefore, irradiation-sensitive cells, presumably immune cells including macrophages, do not primarily mediate the increased in vivo tumor growth induced by locally produced IL-17. IL-17 markedly enhanced tumor microvascularity To investigate the mechanisms by which the in vivo growth of tumors transduced with IL-17 was markedly enhanced, we first performed histologic examination of tumor tissues.Hematoxylin-eosin-stained sections demonstrated that MCA205IL-17 tumors were more infiltrated with neutrophils and were edematous compared with controls (Figure 2A-B). Similar results were found in MC38IL-17 tumors (Figure 2C-D). Immunostaining for Factor VIII showed that tumor tissues of IL-17
transfectants were more markedly vascularized than parental and
Neo-transfected tumors (Figure 2E-H and data not shown). To compare the
vascular density, we determined the mean number of blood vessels of
Factor VIII-stained sections obtained from 5 independent IL-17
transfectants, Neo transfectants, or parental tumors (Figure 2I). The
mean number of microvessels in sections of MCA205IL-17 tumors was
significantly higher than that of controls on days 12 and 23 (P < .001). Similar results were obtained in MC38 tumors
on days 23 and 30 (P < .01).
IL-17 induces neovascularization in rat cornea To confirm that IL-17 is angiogenic in vivo, we first examined the formation of new capillary blood vessels in rat cornea assay. Implants containing 100 ng human bFGF as positive control induced angiogenic response (8 positives of 8 corneas tested) (Figure 3B). Although control implants containing PBS gave no angiogenic response (Figure 3A) (no positives of 8 corneas tested), implants containing 250 or 500 ng mouse IL-17 induced significant angiogenic response (8 positives of 8 corneas tested for each dose) (Figure 3C-D). No signs of accompanying inflammatory reaction were observed as shown by the persistence of cornea transparency for the duration of the experiment. The addition of neutralizing antimouse IL-17 mAb to the implants completely abolished the IL-17-induced vascularization but not control antibody (Figure 3E-F).
Moreover, we attempted to elucidate the angiogenic activity of IL-17 secreted by CD4 T cells. Concentrated conditioned media from CD4 T cells stimulated by PMA and ionomycin were shown to induce significant neovascularization (Figure 3G). The addition of neutralizing antihuman IL-17 mAb to Matrigel resulted in marked reduction in neovessel formation (Figure 3H). These results demonstrate that most of the angiogenic activity present in the conditioned media of CD4 T cells activated in vitro was neutralizable with anti-IL-17 mAb. Effects of IL-17 on vascular endothelial cells We examined the effects of IL-17 on in vitro angiogenesis-related functions of vascular endothelial cells. In contrast to bFGF, a wide range of doses of human IL-17 (0.1-500 ng/mL) had no direct effect on the proliferation of HMVECs and HUVECs (Figure 4A and data not shown). However, as shown in Figure 4B, we observed a dose-dependent chemotactic response of HMVECs toward IL-17. In addition, IL-17 also induced chemotactic response of HUVECs (data not shown). The maximal chemotactic response for HMVECs occurred around 10 ng/mL IL-17. The specificity of this chemotactic response was determined by using neutralizing mAb (20 µg/mL) to human IL-17. IL-17-induced migration of HMVECs (10 ng/mL) was similar to that induced by 15 ng/mL bFGF.
IL-17 markedly promotes endothelial cord formation in vitro We further evaluated the effects of IL-17 on vascular endothelial cell cord formation. Control culture developed small amounts of vessel formation (Figure 5A). VEGF (10 ng/mL) as positive control increased vessel-like structure (Figure 5C). The addition of a wide range of doses of human IL-17 at the start of assay and with each medium change resulted in a marked enhanced development of structures resembling a microvasculature bed (Figure 5B). IL-17-increased microvessel was estimated at more than 60% of the control value (Figure 5E). Around 50 ng/mL IL-17 developed most abundant microvessel-like structures in this assay system.
IL-17 markedly up-regulates production of a variety of proangiogenic factors by fibroblasts as well as tumor cells To examine another potential mechanisms by which IL-17 mediates angiogenesis, we tested whether IL-17 might induce fibroblasts and tumor cells to produce angiogenic growth factors and angiogenic cytokines that had been reported to promote tumor angiogenesis. IL-17 significantly increased elaboration of multiple angiogenic factors, including PGE1,20 PGE2,21 VEGF,22 keratinocyte-derived chemokine (KC),23 macrophage inflammatory protein (MIP)-2,23 and/or NO24 (Tables 2-3). The addition of neutralizing antimouse IL-6 mAb or indomethacin to the culture did not abolish IL-17-induced VEGF production (data not shown), suggesting that this effect was direct. In addition, MCA205IL-17 cells produced more VEGF, KC, and PGE2 in response to IL-17 secreted by themselves than the parental cells and MCA205Neo cells (Table 4). Neutralization of IL-17 inhibited the increased elaboration of these proangiogenic factors (Table 4). In contrast, induction of aFGF,25 bFGF,25 and TGF-26 production was not
observed by semiquantitative RT-PCR (data not shown). These results
indicate that IL-17 promotes angiogenesis in part via induction of a
variety of proangiogenic factors by fibroblasts and tumors.
Here we report for the first time that IL-17 is a novel CD4 T-cell-derived angiogenic factor that plays a potential role in T-cell-mediated neovascularization. Neither recombinant IL-17 protein nor IL-17 gene transduction influenced in vitro tumor growth. However, tumors transduced with IL-17 grew more rapidly than controls in immunocompetent and irradiated mice. Vascular elements of tumor tissues in IL-17 transfectants were significantly increased when compared with those of controls. Because neovascularization is a critical process for the sustained growth of solid tumors, our findings suggest that IL-17 may accelerate in vivo tumor growth via acting as an angiogenic factor. On the basis of these observations, we hypothesized that IL-17 may be a CD4 T-cell-derived stimulator of angiogenesis. To assess the validity of this hypothesis and to elucidate its contribution to the observed enhanced vascularity in situ, we first evaluated the ability of IL-17 to mediate neovessel formation in vivo. Amounts as small as 250 ng IL-17 induced significant corneal angiogenic response with no evidence of nonspecific inflammation. The IL-17 dependency of in vivo angiogenic processes was confirmed by the inhibitory effect of anti-IL-17 mAb. Moreover, there have been reports demonstrating that CD4 T cells induce angiogenesis via elaboration of angiogenic lymphokines.27,28 Therefore, we determined whether IL-17 contributed to the angiogenic activity liberated by human T cells activated in vitro. Importantly, neutralization of IL-17 in the T-cell-conditioned media resulted in significant diminution of the angiogenic response in Matrigel plug assay. These studies indicate that IL-17 is a novel CD4 T-cell-derived mediator of angiogenesis and suggest a role for CD4 T cells as cellular effectors of angiogenesis mediated in part by IL-17. To explore the mechanisms by which IL-17 mediates neovascularization,
we next evaluated the influence of IL-17 on in vitro angiogenesis-related functions of vascular endothelial cells. IL-17 had
no direct effect on the in vitro proliferation of vascular endothelial
cells. However, a wide range of doses of IL-17 (0.05-100 ng/mL)
significantly stimulated the migration. Moreover, in in vitro cord
formation assay, IL-17 markedly promoted the development of
microvessel-like structures. Therefore, IL-17 promotes angiogenesis via
stimulation of vascular endothelial cell migration and cord formation.
Growth factors and cytokines that stimulate angiogenesis in vivo but
fail to demonstrate mitogenic activity for vascular endothelial cells
in vitro have been called indirect angiogenic factors. Therefore, these
characteristics of IL-17 appear to resemble those of the class of
indirect angiogenic stimulators, including TGF- Fibroblasts are a source of proangiogenic factors, including VEGF, during development, hypoxia, tumor growth, and inflammation, and they markedly promote inflammatory and tumor angiogenesis.30,31 We found that IL-17 is able to markedly up-regulate VEGF, KC, MIP-2, prostaglandins, and NO production by fibroblasts. IL-17, therefore, stimulates production of proangiogenic factors in fibroblasts and promotes fibroblast-induced neovessel formation in inflammation and tumors. Although previous studies indicated the involvement of IL-17 in pathologic situations such as rheumatoid arthritis,32-34 the role of IL-17 in pathologic angiogenesis has not been evaluated yet. Our findings suggest that IL-17 functions as a CD4 T-cell-derived angiogenic mediator, now implicated in the pathogenesis of inflammatory angiogenesis. In addition, these findings suggest that some of the angiogenic activities of IL-17 may reflect the indirect effects on vascular endothelial cells mediated by an array of proangiogenic factors produced by fibroblasts. Several investigations about the effects of IL-17 on in vivo tumor growth using other tumor model systems have been reported. Hirahara et al35 and Benchetrit et al36 demonstrated that IL-17 inhibits tumor growth in a T-cell-dependent manner. In contrast, Tartour et al37 reported that IL-17 promotes human cervical tumor growth via IL-6-dependent mechanism. Therefore, it appears that IL-17 may be a pleiotropic cytokine with possible tumor-promoting or tumor-suppressing effects. However, although human cervical tumors are generally poorly immunogenic, murine Meth-A fibrosarcoma, P815, and J558L leukemic cells are immunogenic. In addition, MCA205 and MC38 used in our experiments are weakly and poorly immunogenic tumors, respectively. Thus, the effects of IL-17 on in vivo tumor growth may depend largely on the immunogenicity and cell type of tumors. Macrophages have been thought to play a key role in physiologic and
pathologic angiogenesis.37 Secretory products such as TNF- Although the immune system has been suggested to be an important negative regulator of tumor growth, several experimental models suggest that immune responses could on occasion promote tumor growth.38 In particular, there have been reports demonstrating the deleterious effects of CD4 T cells on tumor immunity in several treatment models.39-41 We show here that the CD4 T-cell-derived cytokine IL-17 has significant tumor-promoting effects via potentiation of tumor angiogenesis. CD4 T cells infiltrating into tumor tissues may stimulate tumor cells and stromal fibroblasts, in conjunction with tumor-associated macrophages, to elaborate a variety of proangiogenic factors, including VEGF. VEGF also influences the immune system via inhibition of the development of dendritic cells42 and subsequently promotes tumor angiogenesis and growth. This could explain in part the reported paradoxical role of CD4 T cells in tumor immunity. In conclusion, our findings illustrate a novel biologic function of CD4 T-cell-derived cytokine IL-17. IL-17 promotes angiogenesis via not only stimulation of vascular endothelial cell migration but also induced elaboration of a variety of proangiogenic factors that lead to the imbalance between angiogenesis activators and inhibitors present within the vascular microenvironment. Investigation of the physiologic regulation of IL-17 production by CD4 T cells may thus be useful for the treatment in clinical settings characterized by persistent neovascularization. Its production by herpesvirus saimiri also suggests that vascular events, including angiogenesis, are important for virus pathogenesis and persistence.
We thank Dr Tatsuro Ishibashi and Dr Michihiko Kuwano for general assistance. We are also grateful to Susan F. Schoonover, Loraine R. McKenzie, and Jennifer Lonellie for their excellent technical assistance.
Submitted May 20, 2002; accepted October 8, 2002.
Prepublished online as Blood First Edition Paper, October 31, 2002; DOI 10.1182/blood-2002-05-1461.
Supported in part by National Institutes of Health grant POICA59371 (H.T. and M.T.L.).
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734.
Reprints: Muneo Numasaki, Department of Geriatric and Respiratory Medicine, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan; e-mail: numasaki77{at}aol.com.
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Abstract
Introduction
, IL-1
B, and regulates the activities of
extracellular-regulated kinase 1 (ERK1), ERK2, c-Jun N-terminal kinase,
and p38 mitogen-activated protein kinases.
5
M 2-mercaptoethanol, 0.1 mM nonessential amino acids (NEAA), 100 IU/mL penicillin, and 100 µg/mL streptomycin (all from Life Technologies, Grand Island, NY), which are designated as complete medium (CM). Human umbilical vein endothelial cells (HUVECs) and human
microvascular endothelial cells (HMVECs) were purchased from Clonetics
(San Diego, CA) and maintained in Endothelial Cell Basal Medium (EBM)
with 12 µg/mL bovine brain extract, 1 µg/mL hydrocortisone, 10 ng/mL epidermal growth factor, 50 µg/mL gentamycin, 50 ng/mL
amphotericin-B, and 2% or 5% FCS (all from Clonetics). Primary
culture of fibroblasts was established from the lung of 7-week-old
C57BL/6 mice. Peripheral blood mononuclear cells were isolated using
Lymphoprep (Nycomed Pharma As, Oslo, Norway) from blood from healthy
volunteers. CD4 T cells were purified using CD4 T-Cell Enrichment
Column (R&D Systems) according to the manufacturer's instructions. CD4
T cells were cultured for 84 hours in RPMI 1640 with 5% FCS, 2 mM
L-glutamine, 1 mM sodium pyruvate, 0.1 mM NEAA, 100 IU/mL
penicillin, and 100 µg/mL streptomycin in the presence of a mixture
of phorbol myristate acetate (PMA) (1 nM) and ionomycin (3 µg/mL)
(both from Sigma, St Louis, MO). Conditioned media of CD4 T cells,
which contained approximately 7.1 ng/mL IL-17, were concentrated 40 times using Centricon (Millipore, Bedford, MA).
