|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
Prepublished online as a Blood First Edition Paper on December 19, 2002; DOI 10.1182/blood-2002-11-3387.
PLENARY PAPER
From the Institute for Cancer Genetics and the
Departments of Pathology and of Genetics and Development, Columbia
University, New York; and the Laboratory of Cancer Genetics and the
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New
York, NY.
The BCL6 proto-oncogene encodes a
transcriptional repressor whose expression is deregulated by
chromosomal translocations in approximately 40% of diffuse large
B-cell lymphomas (DLBCLs). The BCL6 regulatory sequences
are also targeted by somatic hypermutation in germinal center (GC) B
cells and in a fraction of all GC-derived lymphomas. However, the
functional consequences of these mutations are unknown. Here we report
that a subset of mutations specifically associated with DLBCL causes
deregulated BCL6 transcription. These mutations affect 2 adjacent BCL6
binding sites located within the first noncoding exon of the gene, and
they prevent BCL6 from binding its own promoter, thereby disrupting its
negative autoregulatory circuit. These alterations were found in
approximately 16% of DLBCLs devoid of chromosomal translocations
involving the BCL6 locus, but they were not found in normal GC B cells.
This study establishes a novel mechanism for BCL6 deregulation and
reveals a broader involvement of this gene in DLBCL pathogenesis.
(Blood. 2003;101:2914-2923) The BCL6 proto-oncogene was originally
identified because of its involvement in 3q27 chromosomal
translocations associated with diffuse large B-cell lymphomas
(DLBCLs).1-4 BCL6 encodes a POZ/zinc finger
(ZF) transcriptional repressor expressed in mature germinal center (GC)
B cells and required for GC formation and for affinity maturation
during T-cell-dependent antibody responses.5-10 The BCL6
protein acts as a potent transcriptional repressor of promoter
sequences containing its specific DNA recognition
motif.8,11 This function requires DNA binding through the
ZF domain and 2 noncontiguous transrepression domains, including
its N-terminal POZ motif and a second region located in the middle of
the molecule. Because of the similarity between the DNA binding sites
of BCL6 and the signal transducer and activator of transcription 6 (STAT6) transcription factor, BCL6 can modulate incoming interleukin-4 (IL-4) signaling by repressing STAT6-induced transcription from the
immunoglobulin (Ig) germline Consistent with its essential role in the GC reaction, BCL6 expression
is regulated by a number of signals important for GC development and
differentiation. At the protein level, activation of the B-cell
receptor induces mitogen-activated protein (MAP) kinase-mediated
phosphorylation of BCL6, which, in turn, targets BCL6 for rapid
degradation by the ubiquitin proteasome pathway.16 At the
transcriptional level, a variety of stimuli, including CD40 receptor
engagement and mitogenic stimulation, lead to the down-regulation of
BCL6 RNA in cultured B cells.43 More recently, a
novel mechanism has been identified that down-regulates BCL6 activity
by inhibiting its transrepression function through p300-mediated acetylation.17
In tumors, chromosomal translocations affecting the BCL6 locus at band
3q27 represent the most common and specific genetic abnormality
associated with DLBCL, accounting for more than 40% of the
cases.1,18,19 These translocations juxtapose heterologous promoters from the partner chromosome with intact BCL6 coding sequences, leading to deregulated expression of BCL6 by a mechanism known as promoter substitution.20,21
In addition, the 5' regulatory sequences of BCL6 can be
altered by multiple somatic mutations in 30% to 40% of GC B
cells.22-24 These mutations cluster within approximately 2 kb of the transcription initiation site at average frequencies of
1.2 × 10 In the present study, we investigated the functional consequences of
BCL6 mutations by performing a structure-function analysis on 55 mutant alleles (a total of 371 mutations) derived from normal GC
lymphocytes or from various lymphoma subtypes. We identified a subset
of mutations, located within the first noncoding exon of BCL6, that
disrupts the mechanism of negative autoregulation normally involved in
controlling BCL6 expression levels. These mutations were found only in
DLBCL cases, suggesting that they might have been selected during
lymphomagenesis for their ability to deregulate BCL6 expression.
Cell lines, stable transfections, and sorting of tonsillar GC
B cells
DNA extraction and polymerase chain reaction
amplification of the BCL6 promoter region
Cloning and sequence analysis Purified amplicons were sequenced directly from both strands, as described.23 The 2.7-kb BCL6 amplicons were digested with NdeI and SpeI, gel purified using the QIAquick gel extraction kit (Qiagen, Valencia, CA), and cloned into the corresponding sites of the pLA/S5 vector (see "Plasmid constructs") using the Rapid Ligation Kit (Roche); approximately 100 recombinant clones from GC B cells and 6 to 10 clones from each tumor case were analyzed to identify individual alleles. Full-length sequencing of the 2.7-kb BCL6 promoter region was then performed on selected clones to confirm the mutations observed by direct sequencing and to detect additional changes, possibly representing subclonal variants or DNA polymerase-introduced errors. The BCL6 5' noncoding sequence has been deposited in GenBank under accession number AY189709 (note that the numbering has been corrected (+1 bp) compared with that reported in our previous publications23,25,27).Plasmid constructs The original BCL6 luciferase reporter plasmid (pLA/S14wt) was constructed in multiple steps by inserting an approximately 14-kb AvrII/NcoI genomic fragment of the native BCL6 promoter (spanning the 5' flanking sequences, exon 1, intron 1, and exon 2) into the multiple cloning site of the pGL3 vector (Promega, Madison, WI). This construct displayed comparable basal activity to a deletion mutant lacking the approximately 7.7-kb SpeI/Bsu36I fragment at the 3' end of intron 1 (pLA/S5wt; Figure 1A). The latter was therefore used as a backbone to derive all the mutant reporter constructs by exchanging the wild-type 2776-bp NdeI-SpeI region of the BCL6 5' sequences with the corresponding fragment PCR amplified from genomic DNA of GC B cells and lymphoma cases. Four of these constructs (Ly1A, 93-611A, 93-611B, and 93-2889A) were further dissected by swapping in
different combinationsmutant and wild-type fragments corresponding to
the 5' flanking, first exon and first intron of BCL6, as defined by the
restriction sites NdeI ( 694), PmlI (64),
SexAI (+276), and SpeI (+2082). Single point
mutants were generated by site-directed mutagenesis using the pLA/S5wt
plasmid as a target and the Transformer Site-Directed Mutagenesis Kit
(BD Biosciences Clontech, Palo Alto, CA). The expression vector
pMT2T-HA-BCL6 and its deletion mutants pMT2T-HA-BCL6-ZF and
pMT2T-HA-BCL6- ZF have been described previously.8 The metallothionein-inducible expression vector pMEP4-HA-BCL6 was constructed by cloning a 2.4-kb BCL6 cDNA fragment, hemagglutinin (HA)-tagged, into the BamHI site of pMEP4
(Invitrogen).
Transient transfections and reporter gene assays The B-cell lymphoma lines Ly1, MUTU I, and MUTU III (1.5 × 107/sample) were transfected by electroporation at 250 V and 960 µF using a Bio-Rad Gene Pulser apparatus (Bio-Rad Laboratories, Hercules, CA). Equimolar amounts (2 pmol) of wild-type and mutant reporter plasmids were used in each experiment, together with 0.5 pmol plasmid expressing Renilla luciferase (pRL-TK) as a control. Transient transfections into 293T cells were performed by the calcium-phosphate precipitation method,28 using 0.1 pmol of the various reporter constructs and increasing amounts (0.01, 0.02, 0.04, 0.1 pmol) of the effector plasmids (pMT2T-HA-BCL6, pMT2T-HA-BCL6-ZF, and pMT2T-HA-BCL6-ZF). The total amount of transfected DNA was kept constant in each experiment by adding pMT2T
vector sequences to a final amount of 10 µg. The pRL-TK vector (0.15 µg) was also cotransfected as an internal control for transfection
efficiency. Luciferase activities were measured 48 hours after
transfection using the Dual-Luciferase Reporter Assay Kit (Promega),
according to the manufacturer's protocol.
Electrophoretic mobility shift assay Nuclear extracts were prepared from the Ly1 cell line as described, and gel shift analysis was performed using approximately 3 µg extract, the oligoprobes indicated in Figure 3B, and an anti-BCL6 antiserum (N71-1) that recognizes the N-terminus of the BCL6 protein.8Chromatin immunoprecipitation assay The detailed protocol for formaldehyde cross-linking and chromatin immunoprecipitation (ChIP) has been published.29 Briefly, samples (each obtained from 1-2.5 × 107 B cells) were immunoprecipitated overnight at 4°C with 2 µg anti-BCL6 polyclonal antibody N3 (Santa Cruz Biotechnology, Santa Cruz, CA). In each experiment, one sample with no antibody and one sample with an irrelevant antibody (anti-IgG) were included as negative controls for nonspecific binding. Immune complexes were recovered by adding 30 µL blocked salmon sperm DNA/Protein A agarose beads (50% slurry; Upstate Biotechnology, Lake Placid, NY) and incubating the samples for 2 hours at 4°C. Following extensive washing and reverse cross-linking, DNA was purified by phenol-chloroform extraction, ethanol precipitated, and resuspended in 30 µL TE (100 µL for the total input sample, representing 0.5% of the soluble chromatin before immunoprecipitation). Fragments containing the BCL6 exon 1 binding sites were detected by PCR amplification using 2.5 µL of the above DNA preparations as template and 2 different oligonucleotide pairs5'-ACGCTCTGCTTATGAGGA-3' (sense) and 5'-CGGCAGCAACAGCAATAA-3' (antisense) for the B1 fragment (position +39 to +300) and
5'-GGGTTCTTAGAAGTGGTG-3' (sense) and 5'-CAAAGCATTTGGCAAGAG-3'
(antisense) for the B2 fragment (+227 to +421). Two additional primer
sets were designed to amplify control region A, located approximately 1 kb upstream of the transcription initiation site
(5'-ACTAGGACCCACAATGAA-3' and 5'-CGTTTCAAGATCGTTGTA-3'), and control
region D, corresponding to the coding exon 5 (5'-GTGTGCCACAGCAATATC-3' and 5'-TGGCAGTCAGATTTCTGG-3'). Following 32 cycles of amplification, PCR products were resolved on 2% agarose gels and visualized by ethidium bromide staining. The same amplicons were subsequently gel
purified and sequenced directly and after cloning into pGEM-T vector
(Promega) as described.23
Northern blot, Western blot, and reverse transcription-polymerase chain reaction analysis Total RNA was prepared from various B-cell lines and lymphoma cases using the Trizol reagent (Invitrogen). Northern blot and Western blot analyses for BCL6 expression were performed as described.6 For cDNA synthesis, 2 µg RNA was reverse transcribed using oligo(dT)18 primer and SuperScript II reverse transcriptase (Invitrogen). The resultant first-strand DNA was PCR amplified using primers that annealed to BCL6 exons 1 and 2 and was directly sequenced.
Deregulated expression of DLBCL-associated mutant alleles To investigate the consequences of BCL6 mutations on transcriptional regulation of the BCL6 gene, we compared the transcriptional activity of mutant BCL6 alleles derived from normal cells (ie, sorted GC B cells) and lymphoma cells by using a transient transfection/reporter gene assay in 2 lymphoma lines (Ly1 and MUTU I) permissive for BCL6 expression. Initial studies showed that a luciferase reporter gene under the control of the native BCL6 promoter (approximately 14-kb AvrII/NcoI genomic fragment spanning the 5' flanking sequences, exon 1, intron 1, and exon 2) had activity comparable to that of a deletion mutant lacking approximately 7.7 kb at the 3' end of intron 1 (data not shown). Therefore, we used this shorter plasmid (Figure 1A) as a backbone to generate a series of mutant reporter constructs in which the wild-type 2.7-kb NdeI-SpeI fragment was substituted with variant BCL6 sequences amplified from normal GC cells (20 mutated alleles) or from various tumor cases (6 DLBCL, 5 BL, 4 FL, and 5 B-CLL). For each tumor sample, both alleles, designated A and B, were tested. Although mutations were biallelically distributed in 15 cases, the remaining cases displayed one mutated and one wild-type allele (a detailed description of the mutations present in each construct is available in Supplemental data). The basal expression level of the corresponding plasmids (pLA/S5 series) was then evaluated in the transfection/reporter gene assay in Ly1 and MUTUI.Results showed that all the mutant alleles derived from GC cells (n = 20) had a transcriptional activity comparable to that of the wild-type allele, arbitrarily set as 1 (Figure 1B). Similarly, mutant alleles from BL (n = 8), FL (n = 7), and B-CLL (n = 6) cases were also indistinguishable from the wild-type allele in this assay. However, 4 of 12 (33%) DLBCL-associated alleles were significantly and reproducibly overexpressed (4- to 18-fold) in both transfected cell lines (see Figure 1B for representative data with the Ly1 cell line). Of the 4 deregulated alleles, 2 were derived from 2 distinct DLBCL cases (Ly1 and 93-2889), whereas the remaining 2 were derived from a single case (93-611). Interestingly, deregulation was not observed when the same reporters were transfected in a cell line (MUTU III) that lacks BCL6 expression (not shown), suggesting the involvement of the BCL6 protein itself in the observed effect (see below). These results indicate that some mutations associated with DLBCL can transcriptionally deregulate the BCL6 gene. DLBCL-associated deregulating mutations cluster within exon 1 of BCL6 Each of the 4 BCL6 alleles displaying deregulated activity in the transient transfection assay contains multiple base-pair substitutions scattered throughout the first noncoding exon, the first intron, and, in 2 cases, the 5' flanking sequences (Supplemental data). To identify the mutation(s) responsible for DLBCL-associated deregulation, we generated a series of reporter constructs in which wild-type and mutant fragments corresponding to the 5' flanking, first exon, and first intron of each mutated allele were swapped in all possible combinations. The activity of these constructs was then tested using the same transient transfection/reporter gene assay in Ly1 cells. Results in Figure 2 document that, in all 4 alleles, the mutated sequences responsible for transcriptional deregulation were located in exon 1 (see construct B in each panel). Notably, this region only contains a single mutation in the deregulated allele Ly1A (T257C, indicated by an asterisk) or 2 distinct changes in deregulated alleles 93-611A, 93-611B, and 93-2889A. To ascertain which of these changes is responsible for transcriptional deregulation, each was incorporated into the pLA/S5 wt reporter construct by site-directed mutagenesis, and their effect on transcriptional activation was tested in the same assay. Remarkably, a single nucleotide substitution from each deregulated allele (T257C in Ly1A, A260G in 93-611A, T231G in 93-611B, and T234C in 93-2889A) recapitulated the abnormal behavior displayed by the original mutant. These results demonstrate that, within multiply mutated BCL6 alleles, individual mutationsall located
in the first exonwere responsible for the observed deregulation.
Deregulating mutations cluster within 2 BCL6 binding sites The 4 deregulating mutations are clustered in 2 sequence motifs within exon 1 of BCL6. Significantly, both motifs share extensive homology with the preferred DNA-binding sequence of BCL6 polypeptides (Figure 3A, boxed areas; active mutations are in bold and underlined), suggesting that the BCL6 protein may bind and regulate its own promoter region. To test this hypothesis, we examined whether the 2 potential binding sites within exon 1 (called BSE1A and BSE1B) could bind BCL6 in an electrophoretic mobility shift assay (EMSA). Double-stranded oligonucleotide probes corresponding to the wild-type BSE1A (+225 + 244 from the minor BCL6 promoter) and BSE1B (+249 + 268) sequences were incubated with nuclear extracts from the BCL6-expressing cell line Ly1. Figure 3B shows that both probes were associated with a DNA-binding protein complex similar in migration to the one formed by B6BS, a probe representing the canonical BCL6 binding site8 (lanes 1 and 3 in each gel). Supershift analysis using antisera against the N-terminus of BCL-6 (N71-1) confirmed that these complexes contained BCL6 (lanes 2 and 4). The difference in intensities between the BSE1A- and BSE1B-associated bands may reflect differences in the abundance of these complexes or distinct binding affinities of the BSE1A (8 of 9 matches with the core domain of the canonical BCL6 binding site) and BSE1B (100% identity in the 9-bp core) sequences. Notably, oligonucleotide probes carrying the tumor-derived deregulating mutations (m231, m234, m257, and m260) failed to bind BCL6 (lanes 5-8). In addition, it may be relevant that alleles bearing mutations of the high-affinity BSE1B site (ie, Ly1A and 93-611A) were significantly more deregulated than those harboring mutations of the low-affinity BSE1A site (93-611B and 93-2889A) (Figure 1A-B). Thus, the BCL6 protein can bind the exon 1 sequences in vitro and this binding is abrogated by the DLBCL-associated mutations.
To confirm that endogenous BCL6 can bind to its native promoter in
vivo, we performed ChIP assays using an anti-BCL6 polyclonal antibody
(N3) in 2 BCL6-positive cell lines. Ly1 cells carry a mutation in the
BSE1B site of allele A, which confers deregulation in the reporter
assay (Figure 2) and inactivates BCL6 binding in the EMSA (Figure 3B).
The P3HR1 line was used as a control because it contains mutations in
the BCL6 exon 1/intron 1 boundary (172G>C, 380T>A, and 397T>G)
located outside the BSE1A and BSE1B motifs and not associated with
deregulation. Four different oligonucleotide pairs were designed to
amplify genomic fragments corresponding to the relevant exon 1 sequences (products B1 and B2) or to 2 distal control regions (products
A and D) located approximately 1 kb upstream and more than 10 kb
downstream of exon 1, respectively (Figure
4A). The results show that BCL6 binds
specifically to regions B1 and B2, but not to the control regions A and
D, in both BCL6-expressing cell lines (Figure 4B); no product was
obtained using an irrelevant antibody (IgG) or a control line (CB33)
that did not express BCL6 (Figure 4B, right panel). Thus, the exon 1 sequence containing the BSE1A and BSE1B sites represents a specific target for BCL6 binding in vivo.
Based on the EMSA results showing that BCL6 binds to the wild-type, but not mutant, exon 1 sequences, we investigated whether this differential binding can also be detected in vivo by examining the allelic representation of chromatin immunoprecipitates obtained from Ly1 cells (and P3HR1 cells as a control) using the anti-BCL6 antibody. Because all mutations were heterozygous in these lines, the presence of each individual allele could be uniquely identified by PCR sequencing. Figure 4C shows that both alleles are present in the genomic DNA and in the chromatin solution before immunoprecipitation (input), from both Ly1 and P3HR1 (top and middle panels). However, we consistently observed preferential amplification of the wild-type allele in the ChIP product obtained from Ly1 but not from P3HR1 cells (bottom panels). This predominance of the unmutated allele B in the immunoprecipitated chromatin from Ly1 was confirmed by cloning and sequencing the corresponding PCR amplicon, which revealed wild-type sequences in 83% of the clones as opposed to the approximately 50% expected and observed in the control genomic DNA and in the total input (not shown). Taken together, these results demonstrate that the BCL6 exon 1 sequence is a direct physiologic target for BCL6 binding in vivo and that this binding is impaired by the so-called deregulating mutations associated with DLBCL. BCL6 exon 1 binding sites mediate a negative autoregulatory circuit Given that BCL6 is a transcriptional repressor, these data suggested the existence of an autoregulatory circuit in which BCL6 binds its own promoter and inhibits its own transcription. To examine whether BCL6 genes are subjected to this autoregulatory circuit in vivo, we first analyzed endogenous BCL6 expression levels in a BL cell line, Ramos, which had been engineered to constitutively express exogenous BCL6 under the control of a retroviral vector (PINCO). The results shown in Figure 5A indicate that even modest levels of exogenous BCL6 expression (Ramos cells do not tolerate higher levels) lead to a proportional reduction (approximately 40%) in endogenous BCL6 levels.
To exclude that the observed phenomenon may be related to cell culture selection or to clonal variability, we examined the temporal and quantitative responses of endogenous BCL6 transcription in a lymphoblastoid cell line, EREB, transfected with an inducible BCL6 gene vector (pMEP4-HA-BCL6 [MT-BCL6]) or with an empty vector (pMEP4 [MT]) as control. In this system, transcripts from the exogenous BCL6 sequences are driven by the heavy metal-inducible metallothionein promoter30 and can be distinguished from endogenous transcripts because of their lower molecular weights. Stably transfected EREB-MT and EREB-MT-BCL6 cells were analyzed by Northern blot for BCL6 expression at different time points after the addition of CdCl2 to the cell culture medium. As indicated in Figure 5B, endogenous BCL6 transcripts can be clearly detected in the EREB-MT-BCL6 clone at time 0, when very low levels of exogenous BCL6 are present, possibly because of the leaky activity of the MT promoter (line 4). However, CdCl2-mediated induction of exogenous BCL6 caused a progressive decrease in endogenous BCL6 mRNA levels (lanes 5 and 6). Thus, the down-regulation of BCL6 gene expression in the presence of exogenous BCL6 represents a specific and dose-dependent effect of increased BCL6 protein levels, fulfilling the criteria for an autoregulatory circuit. Finally, we examined whether the autoregulatory circuit functions at
the transcriptional level and whether it targets the BCL6 promoter
region. To this end, the pLA/S5 reporter construct containing the
wild-type BCL6 promoter was cotransfected with increasing amounts of a
BCL6 expression plasmid (pMT2T-HA-BCL6) into 293T cells (Figure 5C).
Luciferase activities revealed a strong and dose-dependent repression
of the reporter gene in the presence of exogenous BCL6 (8% residual
activity at 0.1 pmol input) (Figure 5D, solid bars). This effect was
attributed to genuine BCL6-mediated transcriptional repression because
it was not detected when the same reporter was cotransfected with BCL6
deletion mutants that lack either the DNA-binding (pMT2T-HA-BCL6- BCL6 mutations disrupt the BCL6 autoregulatory mechanism To study whether BCL6 autoregulation targets specifically the BSE1A and BSE1B motifs and is disrupted by the 4 DLBCL-associated mutations, we used the same transient transfection/reporter gene assay and tested the degree of resistance of the mutated alleles to this negative feedback loop. To this end, the responses of the 4 deregulated pLA/S5 single mutant reporters (Figure 2) were compared with those of the wild-type construct on cotransfection with increasing amounts of pMT2T-HA-BCL6 in 293T cells. Variable degrees of resistance to exogenous BCL6 repression were reproducibly observed in each of the 4 mutants (Figure 6). This abnormal response was especially evident in the Ly1A-, 93-611A-, and 93-2889A-derived transfectants and, to a lesser extent, in 93-611B. These data indicate that the DLBCL-associated mutations confer resistance to the autoregulatory feedback loop and also establish the BSE1 motifs as the in vivo targets for autoregulation.
Deregulated expression of BCL6 in DLBCL cases carrying BSE1 mutations The results obtained in the transient transfection experiments imply that deregulated alleles escape the negative autoregulatory circuit and are overexpressed, whereas the wild-type allele remains responsive and transcriptionally silent. Consistent with this hypothesis, Northern and Western blot analyses of DLBCL cell lines showed that the Ly1 cells, which carry a BCL6 allele mutated in the BSE1 site, express levels of BCL6 mRNA and protein higher than lines carrying either translocated, non-BSE1-mutated, or wild-type BCL6 alleles (see Figure 7A-B for representative results). Furthermore, an analysis of BCL6 allele-specific transcription by RT-PCR and sequencing revealed that only one allelethe one that carries the mutation in the BSE1
siteis transcribed in Ly1 cells (Figure 7C, top panel). In contrast,
BCL6 is biallelically expressed in the BJAB (Figure 7C, bottom panel)
and P3HR1 (not shown) lymphoma lines, each of which is heterozygous for
nonderegulating mutations. The correlation between exon 1 mutations and
deregulation could not be validated in primary tumors because the
presence of contaminating normal cells makes it difficult to
quantitatively analyze allelic expression in tumor cells by RT-PCR with
accuracy. Nonetheless, these data demonstrate that the deregulating
mutations alter the transcriptional regulation of endogenous
BCL6 genes in native DLBCL cells.
Frequency of deregulating mutations in DLBCL To determine the frequency of BSE1 mutations in DLBCL, we screened by PCR amplification and direct sequencing a panel of 32 DLBCL cases, all cytogenetically characterized, for the presence of mutations affecting the 2 BCL6 binding sites. Our survey revealed that 4 of 25 (16%) samples lacking 3q27 translocations (approximately 13% of all DLBCLs) carry alterations in these sequences; conversely, mutations within the BSE1 sites were not found in cases in which the BCL6 gene had been disrupted by 3q27 abnormalities. These results indicate that mutations affecting the BCL6 exon 1 binding sites are associated with a sizable fraction of DLBCL and appear to be mutually exclusive with BCL6 rearrangements.
Mutations affecting the 5' noncoding region of the BCL6 gene are found in 30% to 40% of GC B cells and in all GC or post-GC-derived B-cell non-Hodgkin lymphomas.22-27 Despite this high frequency, the functional consequences of the mutations in normal and transformed B cells are unknown. The present study elucidates one mechanism by which specific BCL6 mutations associated with DLBCL can deregulate its own expression, and it identifies a negative autoregulatory circuit that controls BCL6 transcriptional activity in vivo. These results have direct implications for the regulation of BCL6 expression in normal and neoplastic B cells and for the role of BCL6 in lymphomagenesis. The first observation emerging from this study is that the pattern of BCL6 mutations can differ among normal GC cells and transformed cells from various GC-derived lymphoma subtypes. In fact, BCL6 mutations affecting the 2 BCL6 binding sites in exon 1 (BSE1A and BSE1B) were found at sizable frequency among DLBCL-derived alleles, but not in GC cells or in indolent lymphomas. Because BCL6 mutations occur physiologically in GC B cells, most likely by the same mechanism that hypermutates IgV sequences,22-24 it is conceivable that their distribution may be stochastically determined in different cells. Thus, the finding of BSE1 mutations specifically restricted to DLBCL suggests a selection for these mutations in association with lymphoma development. Our results also indicate that the basis for this selection is the ability of BSE1 mutations to disrupt the negative autoregulatory mechanism that controls BCL6 expression. The possibility that BCL6 regulates its own expression was previously proposed based on the identification of a BCL6 binding motif in exon 1 that mediates BCL6-dependent transcriptional repression of a reporter plasmid containing a heterologous promoter.31 The present study provides conclusive evidence for the presence of 2 BCL6 binding sites, one of which was not previously reported, and demonstrates that both are required for BCL6-mediated transcriptional repression in the context of the physiological BCL6 promoter region. Most notably, our results show that these 2 sites bind BCL6 in vivo and that this binding is lost in DLBCL-associated mutant alleles. Taken together, these findings are consistent with the existence of a negative autoregulatory feedback loop in which BCL6 regulates its own expression by direct binding and repression of its promoter region. The existence of this mechanism is further supported by a number of independent observations: (1) endogenous BCL6 expression is down-regulated in cell lines (Figure 5A-B) and in transgenic mice (our unpublished results) expressing exogenous BCL6; (2) the expression of truncated BCL6 alleles is increased in mice expressing a nonfunctional BCL6 protein due to inactivation of the BCL6 gene by homologous recombination (Ye et al, 1997, and our unpublished results); (3) the normal BCL6 allele is not expressed in DLBCL cases in which the second allele is deregulated by chromosomal translocation.20 Autoregulatory circuits represent a common, evolutionarily conserved mechanism for the modulation of gene expression and are typically used by genes encoding transcription factors.32 These circuits have homeostatic functions by limiting the range over which expression levels of a gene can fluctuate.32 In mammalian cells, autoregulatory circuits have been described for well-known proto-oncogenes encoding transcription factors that need to be tightly regulated (eg, c-MYC).33,34 In the case of BCL6, loss of proper autoregulation could be especially relevant considering that this protein can repress the transcription of genes that induce cell cycle arrest, apoptosis, and differentiation.13,14 For at least some of these genes, the extent of repression depends on the relative levels of BCL6 versus other transcriptional activators, such as STAT6, which regulate the same genes.12 Thus, aberrantly high levels of BCL6 may lead to constitutive repression of genes necessary for GC exit and further differentiation, thereby contributing to lymphomagenesis. The results herein identify the 2 BSE1 motifs within BCL6 exon 1 as the critical targets for BCL6 deregulation in DLBCL. This notion is supported by several observations: (1) exon 1 sequences spanning the BCL6 binding sites are highly conserved between human and mouse (100% identity over 120 bp; see also Figure 3A); (2) this region is separated from the BCL6 coding exons in most DLBCL cases that carry a 3q27 translocation (approximately 40%); and (3) this region is lost by internal deletions in a small fraction of cases.35-37 Thus, one common consequence of most translocations and DLBCL-associated BSE1 mutations or deletions is the removal of the sequences involved in BCL6 autoregulation. The identification of a fraction of DLBCL (approximately 13% overall, corresponding to 16% of the nontranslocated cases) in which BCL6 expression is deregulated by exon 1 mutations suggests a broader involvement of the BCL6 proto-oncogene in the pathogenesis of DLBCL than previously suspected based on the frequency of chromosomal translocations (40%).1,18,19 In fact, the frequency of DLBCL-associated mutations that alter BCL6 expression may be even higher if we consider that the assay used here can identify deregulated alleles only if the mutations affect constitutive BCL6 expression levels. Thus, tumor-associated mutations that alter the response of the BCL6 gene to regulatory signals may also exist but would not be identified by our assay. This possibility is supported by the observation that, analogous to cases carrying BCL6 translocations, a number of DLBCLs that display BCL6 mutations, but not translocations, are resistant to the physiologic BCL6 down-regulation induced by CD40 signaling (G. Cattoretti et al, manuscript in preparation). The experimental strategies used in this study can be adapted to investigate the effect of mutations on the ability of various signals to down-regulate BCL6 expression. Finally, by identifying an expanded fraction of DLBCL cases carrying deregulated BCL6 expression, these findings have diagnostic and therapeutic implications. The prognostic significance of BCL6 deregulation has been the object of intense controversy, with some studies claiming it as a favorable indicator and others disclaiming the finding.38-42 This controversy can be explained by the observation herein that the fraction of DLBCLs carrying chromosomal translocations, the one considered by the above prognostic studies, does not account for the entire fraction of DLBCLs carrying deregulated BCL6 and that those with deregulating mutations should also be considered. On the therapeutic side, these results have implications for the clinical trials in which BCL6 is under evaluation as a therapeutic target for histone deacetylase inhibitors.17
We thank G. Cattoretti for the isolation of GC B cells, V. Miljkovic and AnaMaria Babiac for excellent assistance in DNA sequencing, and R. Baer for critically reading the manuscript.
Submitted November 8, 2002; accepted November 28, 2002.
Prepublished online as Blood First Edition Paper, December 19, 2002; DOI 10.1182/ blood-2002-11-3387.
Supported by National Institutes of Health grants CA-072699 (R.D.-F.) and CA-34775 and CA-66999 (R.S.K.C.). L.P. is a Special Fellow of the Leukemia and Lymphoma Society of America.
The online version of the article contains a data supplement.
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734.
Reprints: Riccardo Dalla-Favera, Institute for Cancer Genetics, Columbia University, 1150 St Nicholas Ave, New York, NY 10032; e-mail: rd10{at}columbia.edu.
1.
Ye BH, Lista F, Lo Coco F, et al.
Alterations of a zinc finger-encoding gene, BCL-6, in diffuse large-cell lymphoma.
Science.
1993;262:747-750
2.
Ye BH, Rao PH, Chaganti RSK, Dalla-Favera R.
Cloning of bcl-6, the locus involved in chromosome translocations affecting band 3q27 in B-cell lymphoma.
Cancer Res.
1993;53:2732-2735 3. Kerckaert JP, Deweindt C, Tilly H, Quief S, Lecocq G, Bastard C. LAZ3, a novel zinc-finger encoding gene, is disrupted by recurring chromosome 3q27 translocations in human lymphomas. Nat Genet. 1993;5:66-70[CrossRef][Medline] [Order article via Infotrieve].
4.
Baron BW, Nucifora G, McCabe N, Espinosa R 3rd, Le Beau MM, McKeithan TW.
Identification of the gene associated with the recurring chromosomal translocations t(3;14)(q27;q32) and t(3;22)(q27;q11) in B-cell lymphomas.
Proc Natl Acad Sci U S A.
1993;90:5262-5266
5.
Onizuka T, Moriyama M, Yamochi T, et al.
BCL-6 gene product, a 92- to 98-kD nuclear phosphoprotein, is highly expressed in germinal center B cells and their neoplastic counterparts.
Blood.
1995;86:28-37
6.
Cattoretti G, Chang CC, Cechova K, et al.
BCL-6 protein is expressed in germinal-center B cells.
Blood.
1995;86:45-53
7.
Allman D, Jain A, Dent A, et al.
BCL-6 expression during B-cell activation.
Blood.
1996;87:5257-5268
8.
Chang CC, Ye BH, Chaganti RSK, Dalla-Favera R.
BCL-6, a POZ/zinc-finger protein, is a sequence-specific transcriptional repressor.
Proc Natl Acad Sci U S A.
1996;93:6947-6952
9.
Dent AL, Shaffer AL, Yu X, Allman D, Staudt LM.
Control of inflammation, cytokine expression, and germinal center formation by BCL-6.
Science.
1997;276:589-592 10. Ye BH, Cattoretti G, Shen Q, et al. The BCL-6 proto-oncogene controls germinal-centre formation and Th2-type inflammation. Nat Genet. 1997;16:161-170[CrossRef][Medline] [Order article via Infotrieve]. 11. Seyfert VL, Allman D, He Y, Staudt LM. Transcriptional repression by the proto-oncogene BCL-6. Oncogene. 1996;12:2331-2342[Medline] [Order article via Infotrieve].
12.
Harris MB, Chang CC, Berton MT, et al.
Transcriptional repression of Stat6-dependent interleukin-4-induced genes by BCL-6: specific regulation of epsilon transcription and immunoglobulin E switching.
Mol Cell Biol.
1999;19:7264-7275 13. Shaffer AL, Yu X, He Y, Boldrick J, Chan EP, Staudt LM. BCL-6 represses genes that function in lymphocyte differentiation, inflammation, and cell cycle control. Immunity. 2000;13:199-212[CrossRef][Medline] [Order article via Infotrieve].
14.
Baron BW, Anastasi J, Thirman MJ, et al.
The human programmed cell death-2 (PDCD2) gene is a target of BCL6 repression: implications for a role of BCL6 in the down-regulation of apoptosis.
Proc Natl Acad Sci U S A.
2002;99:2860-2865
15.
Fearon DT, Manders P, Wagner SD.
Arrested differentiation, the self-renewing memory lymphocyte, and vaccination.
Science.
2001;293:248-250
16.
Niu H, Ye BH, Dalla-Favera R.
Antigen receptor signaling induces MAP kinase-mediated phosphorylation and degradation of the BCL-6 transcription factor.
Genes Dev.
1998;12:1953-1961 17. Bereshchenko O, Gu W, Dalla-Favera R. Acetylation inactivates the BCL6 transcriptional repressor. Nat Genet. 2002;32:606-613[CrossRef][Medline] [Order article via Infotrieve].
18.
Lo Coco F, Ye BH, Lista F, et al.
Rearrangements of the BCL6 gene in diffuse large cell non-Hodgkin's lymphoma.
Blood.
1994;83:1757-1759
19.
Butler MP, Iida S, Capello D, et al.
Alternative translocation breakpoint cluster region 5' to BCL6 in B-cell non-Hodgkin's lymphoma.
Cancer Res.
2002;62:4089-4094 20. Ye BH, Chaganti S, Chang CC, et al. Chromosomal translocations cause deregulated BCL6 expression by promoter substitution in B cell lymphoma. EMBO J. 1995;14:6209-6217[Medline] [Order article via Infotrieve].
21.
Chen W, Iida S, Louie DC, Dalla-Favera R, Chaganti RS.
Heterologous promoters fused to BCL6 by chromosomal translocations affecting band 3q27 cause its deregulated expression during B-cell differentiation.
Blood.
1998;91:603-607
22.
Shen HM, Peters A, Baron B, Zhu X, Storb U.
Mutation of BCL-6 gene in normal B cells by the process of somatic hypermutation of Ig genes.
Science.
1998;280:1750-1752
23.
Pasqualucci L, Migliazza A, Fracchiolla N, et al.
BCL-6 mutations in normal germinal center B cells: evidence of somatic hypermutation acting outside Ig loci.
Proc Natl Acad Sci U S A.
1998;95:11816-11821
24.
Peng HZ, Du MQ, Koulis A, et al.
Nonimmunoglobulin gene hypermutation in germinal center B cells.
Blood.
1999;93:2167-2172
25.
Migliazza A, Martinotti S, Chen W, et al.
Frequent somatic hypermutation of the 5' noncoding region of the BCL6 gene in B-cell lymphoma.
Proc Natl Acad Sci U S A.
1995;92:12520-12524
26.
Capello D, Vitolo U, Pasqualucci L, et al.
Distribution and pattern of BCL-6 mutations throughout the spectrum of B-cell neoplasia.
Blood.
2000;95:651-659
27.
Pasqualucci L, Neri A, Baldini L, Dalla-Favera R, Migliazza A.
BCL-6 mutations are associated with immunoglobulin variable heavy chain mutations in B-cell chronic lymphocytic leukemia.
Cancer Res.
2000;60:5644-5648 28. Chang CC, Zhang J, Lombardi L, Neri A, Dalla-Favera R. Rearranged NFKB-2 genes in lymphoid neoplasms code for constitutively active nuclear transactivators. Mol Cell Biol. 1995;15:5180-5187[Abstract].
29.
Frank SR, Schroeder M, Fernandez P, Taubert S, Amati B.
Binding of c-Myc to chromatin mediates mitogen-induced acetylation of histone H4 and gene activation.
Genes Dev.
2001;15:2069-2082
30.
Stuart GW, Searle PF, Chen HY, Brinster RL, Palmiter RD.
A 12-base-pair DNA motif that is repeated several times in metallothionein gene promoters confers metal regulation to a heterologous gene.
Proc Natl Acad Sci U S A.
1984;81:7318-7322 31. Kikuchi M, Miki T, Kumagai T, et al. Identification of negative regulatory regions within the first exon and intron of the BCL6 gene. Oncogene. 2000;19:4941-4945[CrossRef][Medline] [Order article via Infotrieve]. 32. Becskei A, Serrano L. Engineering stability in gene networks by autoregulation. Nature. 2000;405:590-593[CrossRef][Medline] [Order article via Infotrieve]. 33. Penn LJ, Brooks MW, Laufer EM, Land H. Negative autoregulation of c-myc transcription. EMBO J. 1990;9:1113-1121[Medline] [Order article via Infotrieve]. 34. Grignani F, Lombardi L, Inghirami G, Sternas L, Cechova K, Dalla-Favera R. Negative autoregulation of c-myc gene expression is inactivated in transformed cells. EMBO J. 1990;9:3913-3922[Medline] [Order article via Infotrieve]. 35. Nakamura Y, Miki T, Miura I, et al. Internal DNA deletion within the BCL-6 gene on untranslocated chromosome in non-Hodgkin's lymphoma with 3q27 abnormality. Leukemia. 1996;10:658-661[Medline] [Order article via Infotrieve]. 36. Nakamura Y, Saito K, Furusawa S. Analysis of internal deletions within the BCL6 gene in B-cell non-Hodgkin's lymphoma. Br J Haematol. 1999;105:274-277[Medline] [Order article via Infotrieve]. 37. Bernardin F, Collyn-d'Hooghe M, Quief S, Bastard C, Leprince D, Kerckaert JP. Small deletions occur in highly conserved regions of the LAZ3/BCL6 major translocation cluster in one case of non-Hodgkin's lymphoma without 3q27 translocation. Oncogene. 1997;14:849-855[CrossRef][Medline] [Order article via Infotrieve].
38.
Bastard C, Deweindt C, Kerckaert JP, et al.
LAZ3 rearrangements in non-Hodgkin's lymphoma: correlation with histology, immunophenotype, karyotype, and clinical outcome in 217 patients.
Blood.
1994;83:2423-2427
39.
Vitolo U, Gaidano G, Botto B, et al.
Rearrangements of bcl-6, bcl-2, c-myc and 6q deletion in B-diffuse large-cell lymphoma: clinical relevance in 71 patients.
Ann Oncol.
1998;9:55-61 40. Barrans SL, O'Connor SJ, Evans PA, et al. Rearrangement of the BCL6 locus at 3q27 is an independent poor prognostic factor in nodal diffuse large B-cell lymphoma. Br J Haematol. 2002;117:322-332[CrossRef][Medline] [Order article via Infotrieve]. 41. Jerkeman M, Aman P, Cavallin-Stahl E, et al. Prognostic implications of BCL6 rearrangement in uniformly treated patients with diffuse large B-cell lymphoma: a Nordic Lymphoma Group study. Int J Oncol. 2002;20:161-165[Medline] [Order article via Infotrieve].
42.
Offit K, Lo Coco F, Louie DC, et al.
Rearrangement of the bcl-6 gene as a prognostic marker in diffuse large-cell lymphoma.
N Engl J Med.
1994;331:74-80 43. Niu H, Cattoretti G, Dalla-Favera R. BCL6 controls the expression of the B7-1/CD80 costimulatory receptor in germinal-center B cells. J Exp Med. In press.
© 2003 by The American Society of Hematology.
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 |
|
 |
|
  J. X. Zhou, C. H. Lee, C. F. Qi, H. Wang, Z. Naghashfar, S. Abbasi, and H. C. Morse III IFN Regulatory Factor 8 Regulates MDM2 in Germinal Center B Cells J. Immunol., September 1, 2009; 183(5): 3188 - 3194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Saito, U. Novak, E. Piovan, K. Basso, P. Sumazin, C. Schneider, M. Crespo, Q. Shen, G. Bhagat, A. Califano, et al. BCL6 suppression of BCL2 via Miz1 and its disruption in diffuse large B cell lymphoma PNAS, July 7, 2009; 106(27): 11294 - 11299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Mendez, J. M. Polo, J. J. Yu, M. Krupski, B. B. Ding, A. Melnick, and B. H. Ye CtBP Is an Essential Corepressor for BCL6 Autoregulation Mol. Cell. Biol., April 1, 2008; 28(7): 2175 - 2186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. B. Ding, J. J. Yu, R. Y.-L. Yu, L. M. Mendez, R. Shaknovich, Y. Zhang, G. Cattoretti, and B. H. Ye Constitutively activated STAT3 promotes cell proliferation and survival in the activated B-cell subtype of diffuse large B-cell lymphomas Blood, February 1, 2008; 111(3): 1515 - 1523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chatterji, S. Unniraman, K. M. McBride, and D. G. Schatz Role of Activation-Induced Deaminase Protein Kinase A Phosphorylation Sites in Ig Gene Conversion and Somatic Hypermutation J. Immunol., October 15, 2007; 179(8): 5274 - 5280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. W. Baron, N. Zeleznik-Le, M. J. Baron, C. Theisler, D. Huo, M. D. Krasowski, M. J. Thirman, R. M. Baron, and J. M. Baron Repression of the PDCD2 gene by BCL6 and the implications for the pathogenesis of human B and T cell lymphomas PNAS, May 1, 2007; 104(18): 7449 - 7454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Duquette, M. D. Huber, and N. Maizels G-Rich Proto-Oncogenes Are Targeted for Genomic Instability in B-Cell Lymphomas Cancer Res., March 15, 2007; 67(6): 2586 - 2594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-W. Chen, X.-T. Hu, A. C. Liang, W.-Y. Au, C.-C. So, M. L. Wong, L. Shen, Q. Tao, K.-M. Chu, Y.-L. Kwong, et al. High BCL6 expression predicts better prognosis, independent of BCL6 translocation status, translocation partner, or BCL6-deregulating mutations, in gastric lymphoma Blood, October 1, 2006; 108(7): 2373 - 2383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. W. van Imhoff, E.-J. G. Boerma, B. van der Holt, E. Schuuring, L. F. Verdonck, H. C. Kluin-Nelemans, and P. M. Kluin Prognostic Impact of Germinal Center-Associated Proteins and Chromosomal Breakpoints in Poor-Risk Diffuse Large B-Cell Lymphoma J. Clin. Oncol., September 1, 2006; 24(25): 4135 - 4142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Gearhart, C. M. Corcoran, J. A. Wamstad, and V. J. Bardwell Polycomb Group and SCF Ubiquitin Ligases Are Found in a Novel BCOR Complex That Is Recruited to BCL6 Targets. Mol. Cell. Biol., September 1, 2006; 26(18): 6880 - 6889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Liso, D. Capello, T. Marafioti, E. Tiacci, M. Cerri, V. Distler, M. Paulli, A. Carbone, G. Delsol, E. Campo, et al. Aberrant somatic hypermutation in tumor cells of nodular-lymphocyte-predominant and classic Hodgkin lymphoma Blood, August 1, 2006; 108(3): 1013 - 1020. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Arguni, M. Arima, N. Tsuruoka, A. Sakamoto, M. Hatano, and T. Tokuhisa JunD/AP-1 and STAT3 are the major enhancer molecules for high Bcl6 expression in germinal center B cells Int. Immunol., July 1, 2006; 18(7): 1079 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Tam, M. Gomez, A. Chadburn, J. W. Lee, W. C. Chan, and D. M. Knowles Mutational analysis of PRDM1 indicates a tumor-suppressor role in diffuse large B-cell lymphomas Blood, May 15, 2006; 107(10): 4090 - 4100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. S. Lossos and D. Morgensztern Prognostic Biomarkers in Diffuse Large B-Cell Lymphoma J. Clin. Oncol., February 20, 2006; 24(6): 995 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. O'Connor, M. L. Heaney, L. Schwartz, S. Richardson, R. Willim, B. MacGregor-Cortelli, T. Curly, C. Moskowitz, C. Portlock, S. Horwitz, et al. Clinical Experience With Intravenous and Oral Formulations of the Novel Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid in Patients With Advanced Hematologic Malignancies J. Clin. Oncol., January 1, 2006; 24(1): 166 - 173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. R. Miles, D. K. Crockett, M. S. Lim, and K. S. J. Elenitoba-Johnson Analysis of BCL6-interacting Proteins by Tandem Mass Spectrometry Mol. Cell. Proteomics, December 1, 2005; 4(12): 1898 - 1909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Whitman, S. Liu, T. Vukosavljevic, L. J. Rush, L. Yu, C. Liu, M. I. Klisovic, K. Maharry, M. Guimond, M. P. Strout, et al. The MLL partial tandem duplication: evidence for recessive gain-of-function in acute myeloid leukemia identifies a novel patient subgroup for molecular-targeted therapy Blood, July 1, 2005; 106(1): 345 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. K. Kelly, O. A. O'Connor, L. M. Krug, J. H. Chiao, M. Heaney, T. Curley, B. MacGregore-Cortelli, W. Tong, J. P. Secrist, L. Schwartz, et al. Phase I Study of an Oral Histone Deacetylase Inhibitor, Suberoylanilide Hydroxamic Acid, in Patients With Advanced Cancer J. Clin. Oncol., June 10, 2005; 23(17): 3923 - 3931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Hoefnagel, R. Dijkman, K. Basso, P. M. Jansen, C. Hallermann, R. Willemze, C. P. Tensen, and M. H. Vermeer Distinct types of primary cutaneous large B-cell lymphoma identified by gene expression profiling Blood, May 1, 2005; 105(9): 3671 - 3678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Li, X. Wang, R. Y.-L. Yu, B. B. Ding, J. J. Yu, X.-M. Dai, A. Naganuma, E. R. Stanley, and B. H. Ye BCL-6 Negatively Regulates Expression of the NF-{kappa}B1 p105/p50 Subunit J. Immunol., January 1, 2005; 174(1): 205 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. W. Baron, J. Anastasi, A. Montag, D. Huo, R. M. Baron, T. Karrison, M. J. Thirman, S. K. Subudhi, R. K. Chin, D. W. Felsher, et al. The human BCL6 transgene promotes the development of lymphomas in the mouse PNAS, September 28, 2004; 101(39): 14198 - 14203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pinte, N. Stankovic-Valentin, S. Deltour, B. R. Rood, C. Guerardel, and D. Leprince The Tumor Suppressor Gene HIC1 (Hypermethylated in Cancer 1) Is a Sequence-specific Transcriptional Repressor: DEFINITION OF ITS CONSENSUS BINDING SEQUENCE AND ANALYSIS OF ITS DNA BINDING AND REPRESSIVE PROPERTIES J. Biol. Chem., September 10, 2004; 279(37): 38313 - 38324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Katzenberger, G. Ott, T. Klein, J. Kalla, H. K. Muller-Hermelink, and M. M. Ott Cytogenetic Alterations Affecting BCL6 Are Predominantly Found in Follicular Lymphomas Grade 3B with a Diffuse Large B-Cell Component Am. J. Pathol., August 1, 2004; 165(2): 481 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Tunyaplin, A. L. Shaffer, C. D. Angelin-Duclos, X. Yu, L. M. Staudt, and K. L. Calame Direct Repression of prdm1 by Bcl-6 Inhibits Plasmacytic Differentiation J. Immunol., July 15, 2004; 173(2): 1158 - 1165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Montesinos-Rongen, D. Van Roost, C. Schaller, O. D. Wiestler, and M. Deckert Primary diffuse large B-cell lymphomas of the central nervous system are targeted by aberrant somatic hypermutation Blood, March 1, 2004; 103(5): 1869 - 1875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Greenwald, J. R. Tumang, A. Sinha, N. Currier, R. D. Cardiff, T. L. Rothstein, D. V. Faller, and G. V. Denis E{micro}-BRD2 transgenic mice develop B-cell lymphoma and leukemia Blood, February 15, 2004; 103(4): 1475 - 1484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Hans, D. D. Weisenburger, T. C. Greiner, R. D. Gascoyne, J. Delabie, G. Ott, H. K. Muller-Hermelink, E. Campo, R. M. Braziel, E. S. Jaffe, et al. Confirmation of the molecular classification of diffuse large B-cell lymphoma by immunohistochemistry using a tissue microarray Blood, January 1, 2004; 103(1): 275 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Jardin, C. Bastard, N. Contentin, F. Parmentier, J.-M. Picquenot, H. Tilly, F. K. Stevenson, and S. S. Sahota Intronic BCL-6 mutations are preferentially targeted to the translocated allele in t(3;14)(q27;q32) non-Hodgkin B-cell lymphoma Blood, September 1, 2003; 102(5): 1872 - 1876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Niu, G. Cattoretti, and R. Dalla-Favera BCL6 Controls the Expression of the B7-1/CD80 Costimulatory Receptor in Germinal Center B Cells J. Exp. Med., July 21, 2003; 198(2): 211 - 221. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 |
 |
 |
|||||||
 |
 |
 |
 |
 |
 |
 |
 |
||
| Copyright © 2003 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
Top
Abstract
Introduction
promoter, thereby modulating isotype
switching toward IgE in vitro and in vivo.
3/bp in the mutated cells.
-estradiol to the culture medium. To generate the
stable EREB-MT and EREB-MT-BCL6 cell lines, EREB cells were transfected
by electroporation with 20 µg pMEP4 (Invitrogen, Carlsbad, CA) or
pMEP4-HA-BCL-6 and were selected in hygromycin (150 µg/mL). For the
induction of exogenous BCL6 expression in the EREB-MT-HA-BCL6 stable
line, 2 µM CdCl2 was added to the culture medium, and
cells were harvested at various time points as indicated in Figure 
