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Blood, Vol. 103, Issue 1, 325-332, January 1, 2004 Return to article Table 3.. Characterization of GeneChip small sample target labeling assay   Cell source     Total RNA used initially     cRNA yield, µg     % of "present" call     3'/5' ratio, GAPDH     N CD4     500 ng     54.2     44.4     2.13     N CD4     500 ng     53.4     47.7     2.84     N CD4*     5 µg     52.2     46.7     1.83     N CD4*     5 µg     54.3     44.9     1.44     Pool-AA1-1     500 ng     51.8     45.4     2.17     Pool-AA1-2     500 ng     49.5     46.6     2.68     Pool-AA1-3     500 ng     50.6     44.1     2.67     Pool-AA2     500 ng     50.6     48.5     3.27     Pool-N1-1     500 ng     53.3     41.9     2.55     Pool-N1-2     500 ng     51.7     44.7     1.92     Pool-N1-3     500 ng     54.8     47.0     1.94     Pool-N2     500 ng     48.9     46.7     2.80   GAPDH indicates glyceraldehyde-3-phosphate dehydrogenase; N, healthy donor. * Standard protocol. Return to article

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