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Blood, 1 April 2004, Vol. 103, No. 7, pp. 2433. This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Anasetti, C. Search for Related Content PubMed Articles by Anasetti, C. Related Collections Related Article in Blood Online Social Bookmarking                   What's this? Next Article  Thrombopoietin and the mysteries of plasmacytoid dendritic cells Dendritic cells (DCs) comprise a diverse system of antigen-presenting cells that are responsible for maintaining self-tolerance, and initiating innate or adaptive immune responses to pathogens, rejection of organ transplants, and graft-versus-host disease. DCs exist in low frequency in living organisms and therefore they are difficult to isolate. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor- (TNF-) cooperate in the generation of human myeloid DCs from CD34+ hematopoietic cells.1 The use of these cytokines for ex vivo expansion has made available large numbers of myeloid DCs for studies of normal and pathologic immune responses and for vaccination trials. Chen and colleagues (page 2547) report that thrombopoietin cooperates with FLT-3 in the generation of plasmacytoid DC precursors from human CD34+ hematopoietic cells. Plasmacytoid DC precursors (also called plasmacytoid monocytes, plasmacytoid T cells, type 2 DC precursors, or type 1 interferon-producing cells [IPCs]) are negative for lineage markers and CD11c, are positive for HLA-DR and CD123 (interleukin-3 [IL-3] receptor -chain), and are CD4 bright. They have plasmacytoid morphology, an oval shape, a round nucleus, and a basophilic cytoplasm. Freshly isolated plasmacytoid DC precursors produce high levels of type I interferons upon stimulation with viruses, including the herpes simplex virus and the human immunodeficiency virus, or bacterial oligodeoxynucleotides.2 Thus, plasmacytoid DC precursors have a critical role in innate immune responses against pathogens. Initial functional experiments with plasmacytoid DC precursors were facilitated by the discovery that their survival depends on IL-3.3 In addition, exposure to CD40 ligand or TNF- induces expression of high levels of the costimulatory molecules CD40, CD80, and CD86, as well as CD83, a marker of mature dendritic cells. Mature plasmacytoid DCs are as potent as mature myeloid DCs at stimulating proliferation of alloantigen-specific naive CD4 T cells. However, following stimulation of Toll-like receptor 7 (TLR-7), plasmacytoid DCs produce interferon-, whereas myeloid DCs produce IL-12. T cells activated by plasmacytoid DCs produce high levels of IL-4 and IL-10, whereas T cells activated by myeloid DCs produce interferon- and IL-2 upon rechallenge. Alloantigen presentation by plasmacytoid DCs promotes the induction of IL-10–producing CD8 T regulatory cells.4 Thus, there are theoretical considerations and initial data for a potential role of plasmacytoid dendritic cells in regulating immunity and tolerance after hematopoietic cell transplantation.5 Chen and collaborators show that plasmacytoid DC precursors obtained from CD34+ cells by ex vivo culture with thrombopoietin and FLT-3 produce interferon- upon activation and with appropriate stimuli differentiate into mature DCs. The mechanism by which thrombopoietin promotes differentiation from CD34+ progenitors into the plasmacytoid DC lineage remains unknown. The discovery by Chen and colleagues will allow the generation of large numbers of IPCs and mature plasmacytoid DCs and facilitate studies of their functions. Claudio Anasetti H. Lee Moffitt Cancer Center and Research Institute References Caux C, Dezutter-Dambuyant C, Schmitt D, Banchereau J. GMCSF and TNF- cooperate in the generation of dendritic Langherans cells. Nature. 1992;360: 258-261.[CrossRef][Medline] [Order article via Infotrieve] Siegal FP, Kadowaki N, Shodell M, et al. The nature of the principal type 1 interferon-producing cells in human blood. Science. 1999;284: 1835-1837.[Abstract/Free Full Text] Grouard G, Rissoan MC, Filgueira L, Durand I, Banchereau J, Liu YJ. The enigmatic plasmacytoid T cells develop into dendritic cells with interleukin (IL)-3 and CD40-ligand. J Exp Med. 1997; 185: 1101-1111.[Abstract/Free Full Text] Gilliet M, Liu YJ. Generation of human CD8 T regulatory cells by CD40 ligand-activated plasmacytoid dendritic cells. J Exp Med. 2002;195: 695-704.[Abstract/Free Full Text] Arpinati M, Chirumbolo G, Urbini B, Perrone G, Rondelli D, Anasetti C. Role of plasmacytoid dendritic cells in immunity and tolerance after allogeneic hematopoietic stem cell transplantation. Transpl Immunol. 2003;11: 345-356.[CrossRef][Medline] [Order article via Infotrieve] CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Thrombopoietin cooperates with FLT3-ligand in the generation of plasmacytoid dendritic cell precursors from human hematopoietic progenitors Wei Chen, Svetlana Antonenko, Joel M. Sederstrom, Xueqing Liang, Anissa S. H. Chan, Holger Kanzler, Bianca Blom, Bruce R. Blazar, and Yong-Jun Liu Blood 2004 103: 2547-2553. [Abstract] [Full Text] [PDF] This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Anasetti, C. Search for Related Content PubMed Articles by Anasetti, C. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?

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