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Blood, 15 January 2005, Vol. 105, No. 2, pp. 697-702. Prepublished online as a Blood First Edition Paper on September 7, 2004; DOI 10.1182/blood-2004-03-1059.
IMMUNOBIOLOGY Dendritic cell-derived IL-2 production is regulated by IL-15 in humans and in miceFrom the Department of Biotechnology and Bioscience, University of MilanoBicocca, Milan; the Laboratory of Tumor Immunology and the Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H. San Raffaele, Milan, Italy; and the Institute for Research in Biomedicine, Bellinzona, Switzerland.
Dendritic cells (DCs) are involved in the initiation and regulation of innate and adaptive immune responses. Several molecular mechanisms regulate these diverse DC functions, and we have previously reported that mouse dendritic cells (mDCs) can produce interleukin-2 (IL-2) in vitro and in vivo, in response to microbial activation and T-cell-mediated stimuli. This property is shared by different DC subtypes, including Langerhans cells. Here we show that, on appropriate stimulation, human DCs, both plasmacytoid and myeloid subtypes, also express IL-2. Interestingly, the production of IL-2 by myeloid DCs is induced by T-cell-mediated stimuli and depends on the presence of IL-15. The key role of this cytokine in regulating IL-2 production was also confirmed in the mouse system. In particular, we could show that DCs from IL-15-deficient mice were strongly impaired in the ability to produce IL-2 after interactions with different microbial stimuli. Our results indicate that DC-produced IL-2 is tightly coregulated with the expression of IL-15.
Dendritic cells (DCs) are antigen-presenting cells (APCs) involved in the initiation and regulation of innate and adaptive immune responses. The key role of DCs in regulating innate responses has been recently investigated, and it has been shown that DCs can activate NK cells in humans and in mice.1,2 With regard to T-cell priming, the unique effectiveness of DCs has been demonstrated in many different experimental systems. The high efficiency of DCs as APCs cannot be attributed to a particular DC-specific surface molecule or secreted molecule; rather, it can be attributed to the high level of expression of cell membrane costimulatory proteins, to the efficient antigen-processing machinery, and to the secretion of cytokines, properties acquired by DCs during the maturation process.3
Recently, we have demonstrated that mouse DCs can produce IL-2 after activation with different inflammatory stimuli.4 The production of this cytokine is tightly regulated and is induced in a narrow timeframe after microbial and T-cell-mediated activation.4 IL-2 production can be observed in vitro and in vivo in different DC subtypes, such as CD8
Antibodies and reagent used for human cells
Lipopolysaccharide (LPS; Escherichia coli 026:B6, used at 1 µg/mL) was obtained from Sigma Chemical (St Louis, MO). Influenza virus (strain A/Beijing/353/89) was kindly provided by I. Julkunen. CpG oligodeoxynucleotide 2216 was obtained from Microsynth (Balgach, Switzerland), and rCD40L was obtained from Alexis Biochemicals (Lausen, Switzerland). Phycoerythrin (PE)-conjugated anti-CD86, PE-conjugated anti-CD80, PE-conjugated anti-CD83, PE-conjugated anti-CD40, PE-conjugated anti-IL-2, and PE-conjugated rat isotype control were obtained from Becton Dickinson (San Jose, CA). Biotin-conjugated anti-CD40L (Serotec, Oxford, United Kingdom) and PE-conjugated streptavidin were obtained from Sigma Chemical. Fluorescein isothiocyanate (FITC)-conjugated anti-CD11c was obtained from DAKO Cytomation (Glostrup, Denmark). PE-conjugated anti-Langerin was obtained from Schering-Plough (Kenilworth, NJ). Recombinant human granulocyte macrophage-colony-stimulating factor (rhGM-CSF) was obtained from (Mielogen, Schering-Plough), rhIL-4 was from Becton Dickinson, rhIL-15 was from R&D Systems (Minneapolis, MN; Serotec, Oxford, United Kingdom), rhIL-13 was from Schering-Plough, and recombinant tumor necrosis factor- Complete RPMI medium consisted of RPMI 1640, 2 mM L-glutamine, 100 U/mL penicillin, 50 µg/mL streptomycin, 50 µM 2-mercaptoethanol, 1 mM sodium pyruvate, 0.1 mM nonessential amino acids (BioWhittaker, Walkersville, MD), and heat-inactivated 10% fetal bovine serum (FBS; Hyclone, South Logan, UT). Generation of monocyte-derived DCs from PBMCs Monocytes were positively selected from peripheral blood mononuclear cells (PBMCs by magnetic bead separation using CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany). The CD14+ cells were cultured in 24-well plates (5 x 105/well) for 5 days in the presence of GM-CSF (800 U/mL)/IL-4 (1000 U/mL) or GM-CSF (800 U/mL)/IL-13 (20 U/mL) or GM-CSF (800 U/mL)/IL-15 (400 U/mL). On day 3, half the supernatant was replenished with fresh complete medium and cytokines. Purification and activation of human CD4+ T cells Purified CD4+ T cells were obtained from PBMCs of the same donors from which we derived the DCs by positive magnetic selection after incubation with beads coated with anti-CD4 antibody (Miltenyi Biotec), following the manufacture's instructions. CD4+ T cells (1 x 106 cells/mL) and autologous monocytes (0.25 x 106 cells/mL), as antigen-presenting cells, were cultured in 24-well plates in RPMI 1640 medium supplemented with 10% heat-inactivated human serum (BioWhittaker) in the presence of phytohemagglutinin (10 µg/mL) (Sigma Aldrich, Milan, Italy) for 3 days. Expression of CD40L was determined by fluorescence-activated cell sorter (FACS) analysis. Analysis of IL-2 production by human MoDCs
Monocyte-derived DCs (MoDCs) generated with GM-CSF and IL-15, GM-CSF and IL-13, or GM-CSF and IL-4 were incubated for 6 hours with TNF- IL-12p70 enzyme-linked immunosorbent assay The presence of IL-12p70 in the supernatants from human DCs obtained in the presence of GM-CSF and IL-4, IL-13, IL-15 and induced to maturation with LPS or J558 CD40L cells was determined with the use of a commercially available kit (Bender MedSystems GmbH, Vienna, Austria) according to the manufacturer's instructions. Purification of human pDCs BDCA4+ cells stained with PE-conjugated anti-BDCA4 (Miltenyi Biotec) were positively selected from PBMCs by magnetic bead separation anti-PE (Miltenyi Biotec), following the manufacture's instructions. The BDCA4+ CD14- cells were then sorted on a FACSVantage (Becton Dickinson). IL-2 transcript in pDCs Plasmacytoid DCs (pDCs) were cultured in 96-well plates (5 x 104/well) in complete RPMI medium in the presence of rhIL-3 (10 ng/mL) for 2, 6, and 16 hours in the presence of CpG, CD40L, or influenza virus, or for 12 hours with influenza virus or with CpG plus 4 hours of CD40L. RT-PCR. mRNA was isolated from the cell samples using the Trizol procedure (Invitrogen, Carlsbad, CA). The mRNA was then converted to whole cDNA with random hexamers (Invitrogen). Specific cDNA was amplified by real-time PCR using specific primers.
Real-time PCR. TaqMan probes for IL-2 and 18S (internal control) (Applied Biosystems, Foster City, CA). The normalization to 18S is the result of 2(- Antibodies and reagents used for mouse cells LPS (E coli 026:B6, used at 10 µg/mL or 50 µg/mouse; Sigma Chemical), CpG 5'-TCC ATG ACG TTC CTG ATG CT-3' (1 µM; Primm, Milan, Italy), and zymosan A (used at 10 µg/mL; Sigma Chemical) were the antibodies used. Biotin-conjugated anti-CD3, biotin-conjugated anti-CD19, PE-conjugated anti-B7.2, PE-conjugated anti-IL-2, PE-conjugated rat isotype control, PE-conjugated anti-CD69, and FITC-conjugated anti-CD11c monoclonal antibodies (Becton Dickinson) were the reagents used. Mice
Pathogen-free C57BL/6 and BALB/c mice were purchased from Harlan-Italy, C57BL/6 IL-15 KO8 were obtain from Taconic (Germantown, NY), and C57BL/6 IL-2R DCs and culture medium for mouse cells
Bone marrow-derived DCs (BMDCs) were obtained from C57BL/6 or C57BL/6 IL-15 KO or C57BL/6 IL-2R IL-2 and IL-12p70 enzyme-linked immunosorbent assay The presence of IL-2 and IL-12p70 in mouse DCs was detected by using the DuoSet kit (R&D Systems, Minneapolis, MN) and following the manufacturer's recommendations. In vivo analysis of IL-2 production by mouse DCs C57BL/6 WT or IL-15 KO mice were intraperitoneally injected with 50 µg LPS. Three hours after treatment, spleens were removed, unicellular suspensions were made, and CD11c+ cells were enriched by negative selection of T and B cells. These cells were eliminated with the Dynal Simply magnetic system (Dynal AS, Oslo, Norway). The CD11c+ cells were then sorted on a MoFlo (DAKO Cytomation, Glostrup, Denmark). We incubated the CD11c+ cells with Brefeldin A (10 µg/mL; Sigma Chemical) for 1.5 hours. Cells were then fixed with 2% paraformaldehyde, permeabilized with PBS containing 5% FCS and 0.5% saponin, and stained. Cells were analyzed with the use of FACScan (Becton Dickinson). Mixed lymphocyte reaction BMDC WT or IL-15 KO (2.5 x 105) were activated with zymosan A for 2 hours, and 4 to 7 hours later they were incubated with 1 x 106 carboxyfluorescein diacetate succidimyl ester (CFSE)-labeled T cells. CD4+ and CD8+ lymphocytes were purified (to 99% and 95% purity, respectively) from BALB/c lymph nodes by negative selection of macrophages, DCs, B cells, and CD4+ or CD8+ T cells. These cell populations were eliminated with the Dynal Simply magnetic system (Dynal AS) after preincubation with Mac1, CD11c, CD19, and CD4 or CD8 antibodies (all from Becton Dickinson). T-cell division was assessed through FACS analysis 48 or 72 hours later.
Human MoDCs generated in the presence of IL-15 produce IL-2 after CD40-mediated stimulation
MoDCs were generated in vitro from human peripheral blood CD14+ cells by using 3 different procedures that yielded DCs functionally similar in their ability to prime T cells:10-12 in the presence of GM-CSF and IL-410; in the presence of GM-CSF and IL-1311; or in the presence of GM-CSF and IL-15.12 These MoDCs were equally able to undergo maturation after interaction with LPS and CD40L, as assessed by the up-regulation of activation markers, such as CD80, CD86, and CD83 (Figure 1A). Therefore, we tested the ability of MoDCs to produce IL-2 after stimulation with LPS, CD40L, and LPS plus CD40L. To potentially increase DC activation13 and to create a physiologic inflammatory environment, a cocktail of cytokines composed of IL-6, TNF-
The difference in IL-2 production was not caused by a differential expression of CD40. In fact, the expression of CD40 on MoDCs generated in the various culture conditions was comparable (Figure 1C). In contrast, Langerin was weakly expressed (up to 30% of MoDCs only when generated in the presence of IL-15) (Figure 1C). The lack of ability to produce IL-2 was not attributable to a general impairment. Indeed MoDCs generated with IL-13 and IL-4 produced high amounts of IL-12p70 after LPS or CD40L stimulation (Figure 1D). Interestingly, MoDCs generated with IL-15 did not produce IL-12p70 after maturation with LPS or with CD40L (Figure 1D). To test a more physiologic stimulation, we used activated CD4 T cells, which express CD40L (Figure 1E), to induce IL-2 production by MoDCs derived in the presence of IL-15. As expected, DCs were able to produce IL-2 (Figure 1E) with an even higher percentage of MoDC IL-2+ compared with stimulation with the J558 CD40L-expressing cells (Figure 1B). In conclusion, only MoDCs generated with IL-15 acquired the ability to produce IL-2 after CD40L stimulation. DCs from IL-15-deficient mice produce a reduced level of IL-2 but are still functional
Because mouse DCs produce IL-15 early after activation with inflammatory stimuli,14 we investigated whether the presence of IL-15 is required in the mouse system to render DCs capable of producing IL-2 after activation. We generated BMDCs from IL-15- or IL-2R
The observed difference in IL-2 production was not caused by a defect in mutant DC maturation because no difference in the expression of B7.2 was observed after zymosan A exposure (Figure 2B). Thus, this phenomenon is not attributed to an inability to undergo maturation. The relevance of IL-15 in DC production of IL-2 was also tested in vivo. IL-15-deficient or WT mice were injected with LPS, and, 3 hours later, CD11c+ DCs were sorted from spleens to obtain a pure population (98%) of DCs. LPS was used because it was previously shown to be the best stimulus to induce IL-2 production in vivo.4 Less than 2% of DCs from IL-15-deficient mice produced IL-2, whereas up to 7% of DCs from WT mice were IL-2 positive (Figure 2C), as assessed by intracellular staining. This result was further confirmed by testing cytokine production from the same sorted cells. In contrast, the absence of IL-15 in the KO mice did not affect the ability to produce other cytokines, such as IL-12p70, in response to the same stimulus (LPS) (Figure 2C). As a whole, these results indicate a relevant role of IL-15 in mouse DCs. To evaluate whether DCs from IL-15-deficient mice were functionally responsive, we performed an allogeneic mixed lymphocyte reaction. As shown in Figure 3A, BMDCs from WT and IL-15 KO mice could induce CD4+ and CD8+ T-cell proliferation. The lateness of IL-15-deficient mice in inducing T-cell proliferation was not caused by the difference in BMDC maturation (Figure 3B) or in T-cell activation (Figure 3C), but it could have been caused by an absence of IL-15 and a reduced production of IL-2 by DCs.
Human pDCs transcribe high levels of IL-2 mRNA after stimulation We then tested whether human pDCs were able to produce IL-2 after stimulation. The interferon-producing cells (IPCs), renamed pDCs, exerted potent antiviral activity because of the large amounts of type 1 IFN produced in response to viruses and microbial stimuli, including bacterial DNA.15 pDCs express different cytokines and different toll-like receptors (TLRs) than MoDCs. In particular, MoDCs express TLR 2, 3, 4, and 5, whereas pDCs express TLR 7, 8, and 9.16 We purified pDCs from human blood using a double-selection procedure, first by magnetic bead purification and second by FACS sorting, to obtain a pure population (98%) of pDCs. In these populations, no T-cell contamination was present, as indicated by anti-CD3 FACS analysis (Figure 4D).
By real-time PCR, we observed that human pDCs, after 2-hour stimulation with influenza virus, transcribed IL-2 mRNA and that the transcript level increased with time (after 6 and 16 hours of stimulation), whereas using CpG, IL-2 mRNA expression could be detected only after 6 hours (Figure 4A). In contrast, pDC stimulation with CD40L did not induce IL-2 transcription unless the cells were previously activated with CpG or influenza virus for 12 hours and then were challenged with CD40L for another 4 hours. Thus, reactivation with CD40L induced a second wave of IL-2 transcription in cells pretreated with CpG (Figure 4B), though there was no modification of the quantity of IL-2 transcript if the cells were pretreated with influenza virus, probably because of saturation of the signal. Figure 4C shows that activated T cells that produce the IL-2 protein have a major quantity of IL-2 mRNA compared with pDCs.
In this study we have investigated whether, as in mouse DCs, human MoDCs can produce IL-2 and what signals are required for such activation. We found that MoDCs generated in the presence of IL-15, but not MoDCs generated in the presence of IL-4 and IL-13, can express IL-2 on activation with CD40L. In the mouse system, only immature DCs acquire the ability to produce IL-2 after activation, and, according to this observation, Langerhans cells efficiently produce IL-2 on LPS encounter.4 MoDCs generated with IL-15 show Langerhans cell-like characteristics, such as Langerin expression12 (Figure 1C). Moreover, they show a lower expression of CD83 after activation with LPS plus CD40L compared with MoDCs derived with IL-4 and IL-13, suggesting the acquisition of a less-mature phenotype, which could also explain their lack of IL-12 production.
In human MoDCs generated with IL-15, IL-2 expression is obtained with T cell-derived signals, such as CD40L. Thus, in human MoDCs, contact with T cells plays a key role, whereas in mouse DCs, we have demonstrated not only that T cell-mediated stimuli but also that microbes alone can induce IL-2 production.4 Thus, IL-2 expression seems to be more tightly regulated by human DCs than by mouse DCs. IL-2 production by human DCs during T-cell contact of activated T cells (the expression of CD40L is fundamental) could have a role in CD8+ T-cell activation or could induce regulation of the immune response because IL-2 is involved in apoptosis.17 In the mouse, DCs produce IL-2 after microbial stimulation, and T-cell contact is not necessary. Thus, IL-2 can activate cells in the infected tissue, such as NK cells, before they migrate to the lymphoid organs. Moreover, it is likely that, in pDCs, the regulation of IL-2 production is not achieved by a rapid turnover of the mRNA, as in T cells, but by post-transcriptional modifications in which IL-15 may play a fundamental role. This also appears to be relevant in mouse DCs because IL-15-deficient cells produce low levels of IL-2. This phenomenon appears not to be caused by an inability to undergo maturation, because we were able to induce full phenotypic and functional maturation of the activation of mutant DCs after encounters with different microbial stimuli. This finding is in contrast to that of a previous work in which DCs derived from IL-15-deficient mice failed to undergo functional maturation or to produce IL-12p70 after activation.18 The IL-15R
IL-15 can be produced by human and mouse LPS-activated macrophages, by mouse intestinal epithelial cells on their infection by Listeria monocytogenes,20 in human skin after ultraviolet B irradiation,21 after infection with Mycobacterium leprae, Cryptococcus neoformans, Candida albicans, herpesvirus 6, herpesvirus 7, and hepatitis C.22 Moreover, it has been shown that IL-15/IL-15R
IL-15 and IL-2 are connected to each other by sharing a number of biologic properties: they activate NK, T, and B cells, they induce their proliferation and survival,8,24 and they stimulate cytokine secretion and immunoglobulin synthesis.25,26 For signal transduction, IL-2R and IL-15R share the
Functional synergy between these 2 cytokines has been demonstrated during T-cell activation.31 Indeed, during in vivo activation, T cells are responsive to IL-15, when the cells express the low-affinity IL-2 receptor. Later, when T cells express the high-affinity IL-2, IL-15, and their receptors are potential targets for immunotherapy.28,32 IL-2, through its contribution in activation-induced cell death (AICD) for CD4+ cells, and its interference with persistence of CD8+ memory T cells, plays a critical role in the maintenance of peripheral self-tolerance. In contrast, IL-15, through its inhibition of IL-2-mediated AICD29, and its positive role in the maintenance of CD8+ memory T cells, plays an important role in the preservation of acquired immune responses to foreign pathogens. However, this role of IL-15 carries with it the risk, for the organism, of the survival of self-reactive T cells that could lead to the development of autoimmune diseases. Thus, because the production of IL-15 is critical for the production of IL-2 in mouse and human DCs, this regulation may have a role in modulating the immune response and in reducing the risk for autoimmune diseases.
We thank J. P. M Van Meerwijk and G. Natoli for helpful discussion and Dr F. Brière (Dardilly, France) for rhIL-13 and anti-Langerin.
Submitted March 23, 2004; accepted August 23, 2004.
Prepublished online as Blood First Edition Paper, September 7, 2004; DOI 10.1182/blood-2004-03-1059.
Supported by the Ministry of Education, University and Research (grants FIRB and COFIN); a European Molecular Biology Organization (EMBO) short-term fellowship, and the Italian Association for Cancer Research (AIRC).
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734.
Reprints: Paola Ricciardi-Castagnoli, Department of Biotechnology and Bioscience, University of Milano-Bicocca, Piazza della Scienza 2, Milan, Italy; e-mail: paola.castagnoli{at}unimib.it.
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Abstract
Introduction
+ and CD8
, rhIL-6, and rhIL-3 were from R&D Systems. 
CT), where 
knockout (KO) mice were kept in pathogen-free conditions and were used at 6 to 10 weeks of age. All experiments were performed in compliance with the relevant laws and institutional guidelines. 
