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Blood, 1 June 2006, Vol. 107, No. 11, pp. 4199.
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HEMOSTASIS
Comment on Mangin et al, page 4346
GPVI: no magic bullet for thrombosis
Mark L. Kahn
UNIVERSITY OF PENNSYLVANIA HEALTH SYSTEM
The platelet collagen receptor GPVI has been considered an attractive therapeutic target to treat human cardiovascular diseases. An article by Mangin and colleagues in this issue of Blood reveals that loss of GPVI does not produce the expected dramatic effect on arterial thrombosis in mice.
Platelet activation following plaque rupture is thought to initiate formation of the arterial thrombi that underlie stroke and myocardial infarction. Accordingly, antiplatelet therapies have become a mainstay of both chronic and acute treatment of these diseases. The ideal antiplatelet agent has been elusive, however, as agents that completely disable platelet aggregation (such as the  IIb III receptor antagonists) confer too great a bleeding risk to be safe chronic therapies, while milder agents that block secondary signaling pathways (such as ADP antagonists and aspirin) are better tolerated but not as effective. The identification of the platelet collagen receptor glycoprotein VI (GPVI) immediately raised hopes for the creation of an ideal antiplatelet therapy because of the receptor's narrowly defined thrombotic role,1 as well as the observation that GPVI-deficient individuals manifest only a mild bleeding phenotype.2
Although earlier studies suggested that GPVI might indeed be the target for a platelet magic bullet,3 Mangin and colleagues have popped this therapeutic bubble. Using 3 different models of arterial thrombosis in the mouse, they find that loss of GPVI alone has a minimal protective effect (significantly less than that of the ADP receptor antagonist clopidogrel) that can, however, be enhanced by simultaneous thrombin inhibition. Several important questions emerge from this study. First, can we gauge the potential therapeutic value of inhibiting GPVI or any other platelet receptor on the basis of arterial thrombosis models in the mouse? While this question will remain open, Mangin and colleagues directly compare established pharmacologic inhibitors of integrin IIbIII, the P2Y12 ADP receptor, and thrombin to genetic loss of GPVI, and they observe relative inhibitory effects of these agents that correspond roughly to their efficacy in human trials. Thus, GPVI deficiency appears to have less effect than these agents on acute arterial thrombosis. However, GPVI blockade might be most clinically useful as chronic, prophylactic therapy, an end point not addressed by these studies. Second, do these findings suggest that the role of platelet collagen responses in arterial thrombosis has been overestimated? There is no "relative standard" by which to judge the predictive power of mouse models for pathogenic or physiologic platelet responses in humans, and so this question must remain open. In addition, platelets express 2 collagen receptors, GPVI and the integrin 21. 21 is essential for adhesion of platelets to collagen under flow but requires inside-out signals to engage collagen.4 The authors do not directly test the role of 21, but suggest that it may be minimal because the loss of collagen adhesion in GPVI-deficient platelets cannot be compensated for by 21 ex vivo. However, in an ex vivo assay using anticoagulated blood, the only potential activating signal for integrin 21 is GPVI, while in vivo, 21 may be activated by other stimuli such as thrombin. Thus, there also remains the possibility of functional collagen receptor redundancy in vivo. Finally, the authors suggest that simultaneous targeting of both thrombin and GPVI signaling may provide synergistic blockade of arterial thrombosis. This is an intriguing finding that hints at the power of exploiting signaling cross talk therapeutically. 
References
- Penz S, Reininger AJ, Brandl R, et al. Human atheromatous plaques stimulate thrombus formation by activating platelet glycoprotein VI. FASEB J. 2005;19: 898-909.[Abstract/Free Full Text]
- Moroi M, Jung SM, Okuma M, Shinmyozu K. A patient with platelets deficient in glycoprotein VI that lack both collagen-induced aggregation and adhesion. J Clin Invest. 1989;84: 1440-1445.[Medline]
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- Massberg S, Gawaz M, Gruner S, et al. A crucial role of glycoprotein VI for platelet recruitment to the injured arterial wall in vivo. J Exp Med. 2003;197: 41-49.[Abstract/Free Full Text]
- Sarratt KL, Chen H, Zutter MM, Santoro SA, Hammer DA, Kahn ML. GPVI and alpha2beta1 play independent critical roles during platelet adhesion and aggregate formation to collagen under flow. Blood. 2005;106: 1268-1277.[Abstract/Free Full Text]
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Related Article in Blood Online:
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Thrombin overcomes the thrombosis defect associated with platelet GPVI/FcR
 deficiency
- Pierre Mangin, Cindy L. Yap, Christelle Nonne, Sharelle A. Sturgeon, Isaac Goncalves, Yuping Yuan, Simone M. Schoenwaelder, Christine E. Wright, Francois Lanza, and Shaun P. Jackson
Blood 2006 107: 4346-4353.
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