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Blood, 15 January 2006, Vol. 107, No. 2, pp. 844-845.
CORRESPONDENCE To the editor:Lack of IKBA coding region mutations in primary mediastinal large B-cell lymphoma and the host response subtype of diffuse large B-cell lymphomaThe role of inhibitor of kappa B (IKBA) mutations in lymphoid malignancies with constitutive NF- B signaling remains to be defined. We recently characterized the molecular signatures of primary mediastinal large B-cell lymphoma (MLBCL) and 3 subtypes of diffuse large B-cell lymphoma (DLBCL).1,2 The primary MLBCL signature had striking similarities to that of classic Hodgkin lymphoma (cHL), a clinically related disorder.1,3 Like cHL, primary MLBCL exhibited nuclear localization of the c-REL subunit of NF-B and increased expression of multiple NF-B target genes.1,4 In addition, MLBCL cells transduced with an IB superrepressor exhibited markedly decreased NF-B activity and significantly increased apoptosis, confirming the role of IB and the NF-B pathway in MLBCL cell survival.4 Of interest, the newly identified host response (HR) subtype of DLBCL also had significantly increased coordinate expression of multiple NF-B target genes, implicating the NF-B survival pathway in this additional subtype of LBCL.2,4
Previous studies suggest that DLBCLs that share features with normal in vitro activated B cells ("ABC-like" tumors) also exhibit NF-
In earlier analyses of potential genetic bases for constitutive NF- To determine whether IKBA mutations were present in primary MLBCLs or HR DLBCLs, we subjected 26 MLBCL and 16 HR DLBCL RNAs to reverse-transcriptase-polymerase chain reaction (RT-PCR) of the entire coding region of IKBA cDNA and sequenced the resulting IKBA PCR products. Only 2 single nucleotide changes in the IKBA coding region (bp 95-1048) were identified (C to T, position 175; and C to T, position 400); neither nucleotide change altered the IKBA coding sequence.10 Both of the identified single nucleotide changes are recognized IKBA single nucleotide polymorphisms (SNPs; SNP IDs: rs1957106 and rs10782383) (http://www.ncbi.nlm.nih.gov/SNP). The polymorphism at position 175 was detected in 7 of 16 HR tumors, 2 of which were homozygous (T/T), and 12 of 26 MLBCLs, all of which were heterozygous (T/C). The polymorphism at position 400 was detected in 16 of 16 HR tumors, 7 of which were homozygous (C/C), and 25 of 26 MLBCLs, 16 of which were C/C. To exclude the possibility that infiltrating normal cells in primary MLBCLs and HR DLBCLs reduced the sensitivity of the assay, we identified the previously described IKBA mutation in L428 lymphoma cells8 using L428 RNA admixed with 4-fold higher concentrations of RNA from a cell line with wild-type IKBA (DHL6).
Taken together, our data and that of Thomas et al9 indicate that mutations of IKBA are largely absent in LBCL subtypes, including MLBCL, HR DLBCL, and the differentiation-associated "ABC-like" DLBCLs. These results suggest that alternative mechanisms are likely responsible for the constitutive activation of NF-
Correspondence: Margaret A. Shipp, Dept of Medical Oncology, Dana-Farber Cancer Institute, 44 Binney St, Boston, MA 02115; e-mail: margaret_shipp{at}dfci.harvard.edu.
References
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| Copyright © 2006 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
