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Blood, 1 May 2007, Vol. 109, No. 9, pp. 3617-3618.
ProTECtion from posttransplantation immune deficiency?STANFORD UNIVERSITY
Graft-versus-host disease is associated with poor thymic function and immune reconstitution after hematopoietic stem cell transplantation. Hauri-Hohl and colleagues have elucidated the mechanism by which GVHD damages the thymus.
Hauri-Hohl and colleagues have advanced the field significantly by establishing the immunologic mechanisms by which alloreactive T cells recognize and attack the thymus (see figure). Thirty years ago, Seemayer et al first proposed that the thymus may be a target of GVHD after demonstrating infiltration of the thymus by donor T lymphocytes in experimental murine models.4 Key to the present studies were analyses of both in vivo and in vitro murine models of thymopoiesis and allogeneic transplant that demonstrate that even unprimed T cells can recognize allogeneic antigens presented by thymic epithelial cells (TECs). After allogeneic recognition of the TECs, the donor T cells secrete interferon-gamma (IFN-
The destruction of TECs by alloreactive T lymphocytes may have implications for allogeneic HSCT beyond leading to immune deficiency from prolonged defects in T lymphopoiesis. A recently recognized function of the medullary TEC subset in the development of central tolerance is the negative selection of thymocytes that might develop into autoreactive T lymphocytes. A primary disease of immune development and regulation, the autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) syndrome, is due to loss-of-function mutations of the autoimmune regulator (Aire) gene that controls expression and presentation of self-antigens by medullary TECs.5 While it has been generally assumed that donor-derived T cells that develop in the host thymus after HSCT are host tolerant, the destruction of medullary TECs could lead to defects in central tolerance and consequent autoimmunity. Thus, the destruction of TECs as a result of acute GVHD may provide an etiologic link between the alloreactivity of acute GVHD and the autoimmunity typically seen in chronic GVHD. GVHD has traditionally been assessed and graded in the skin, gut, and liver. As shown by Hauri-Hohl et al, the ability of TECs to present antigen is a mechanism by which GVHD in the thymus could occur without any overt clinical acute GVHD of the typical sites. The occurrence of thymic GVHD without concomitant skin, gut, or liver disease could be a confounder of clinical studies aimed at preventing either GVHD or posttransplantation immune deficiency. For example, is the increased risk of infection and poorer immune reconstitution in URD HSC transplant recipients without overt GVHD due to undiagnosed thymic GVHD?1 In contrast to the tools available for the diagnosis of skin, gut, or liver acute GVHD (physical examination, biomarkers of inflammation, biopsy, endoscopy), routine diagnosis of acute GVHD of the thymus is not readily available. The development of minimally invasive tools for diagnosis of thymic GVHD (eg, imaging or biomarker based) may be a methodologic necessity to assess thymic GVHD. Interventions to improve immune reconstitution via specific protection of TECs may be both necessary and feasible. One candidate strategy would use keratinocyte growth factor (KGF), which has been shown to protect TECs from chemotherapeutic, radiotherapeutic, and allogeneic injury after HSCT, and to stimulate TEC regeneration in aging mice.6–8 The elegant mechanistic studies by Hauri-Hohl et al should stimulate the identification of other potential targets, such as T-cell entry into the thymus or interferon-induced Stat-1 activation in TECs.
Footnotes
Conflict-of-interest disclosure: The author declares no competing financial interests.
REFERENCES
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| Copyright © 2007 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||||
), which activates a STAT-1–induced apoptotic pathway in the TECs. The authors propose that the unique biology of TECs as intrathymic presenters of self-antigen to developing thymocytes allows them to present antigen and prime allogeneic immune responses, even in the absence of typical hematopoietically derived professional antigen-presenting cells (APCs). Although APC depletion has been proposed as a strategy to decrease acute GVHD, the authors' results indicate that this would not prevent the development of thymic injury. 
