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Erratum for Colla et al., Blood 110 (13) 4464-4475. Blood, 1 September 2008, Vol. 112, No. 5, pp. 2170. This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                   What's this?  Previous Article  |  Table of Contents ERRATUM Colla et al. The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 (HIF-1) activity: involvement in myeloma-induced angiogenesis. Blood. 2007;110:4464-4475. On page4464 in the December 15, 2007, issue, Figure 4 was incorrect. The correct figure and its legend are shown. View larger version (32K): [in this window] [in a new window]   Figure 4. Effect of HIF-1alpha suppression on ING4 and the angiogenic-related molecules in hypoxic condition. JJN3 was transfected by electroporation with 1 nmol smart pool double-stranded RNA oligonucleotides (siRNA) against HIF-1alpha or with a nonspecific control siRNA (Cy). After 24 hours, cells were incubated in the presence or absence of hypoxic condition (1% O2, 5% CO2 atmosphere or CoCl2 treatment) for 12 hours. HIF-1alpha mRNA expression was evaluated by RT-PCR, whereas HIF-1alpha protein level and activity were detected by Western blot and ELISA, respectively, as described in "Western blot analysis" and "ELISAs." Graphs represent the means plus or minus SD of 2 independent experiments measured in triplicate (OD = optical density). Nuclear extracts of COS-7 treated with CoCl2 in the presence or absence of wild-type (wt) or mutated (mt) competitor oligonucleotides were tested as control (Con) (A). Aliquots of conditioned media of JJN3 transfected cells were tested by ELISA to detect IL-8 and OPN levels. Graphs represent the means plus or minus SD of IL-8 or OPN levels in 2 independent experiments measured in triplicate (B). JJN3 was transfected by electroporation with 1 nmol smart pool double-stranded RNA oligonucleotides (siRNA) against HIF-1alpha or ING4 or HIF-1alpha plus ING4 or with a nonspecific control siRNA (Cy) and incubated after 24 hours in the presence or absence of hypoxic condition. Thereafter ING4, IL-8, OPN, HIF-1alpha, and NIP-3 mRNA expression was quantified by realtime PCR. Data are expressed as mean –deltaCt's plus or minus SD as described in "Real-time quantitative PCR" (C).   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                   What's this?

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