|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
Blood, 4 June 2009, Vol. 113, No. 23, pp. 5698-5699.
Close encounters of the 3D kindUNIVERSITY OF BERN
The small GTPase Cdc42 is a key regulator of cell polarity. In this issue of Blood, Lämmermann and colleagues show that DCs without Cdc42 are still able to migrate fairly efficiently on 2-dimensional surfaces but become irreversibly entangled in 3-dimensional environments, both in vitro and in vivo.
Small GTPases of the Rho and Ras families are key components of the induction and maintenance of a polarized phenotype and migration. Rac and Rho are involved in lamellipodia formation and uropod retraction, respectively. The Rho family member Cdc42 plays a role in induction and maintenance of polarity in various cell types, such as neutrophils and macrophages, in part through stabilization of the leading edge lamellipodia.2 It remains unclear, however, how Cdc42 affected DC motility. In this issue of Blood, Lämmermann et al report their findings on the role of Cdc42 during physiologic DC migration obtained in a series of elegant in vitro and in vivo assays.3 Using primary mouse DCs derived from Cdc42-deficient bone marrow cultures, the authors investigate the migratory properties of these cells on 2-dimensional surfaces. Despite defects in maintaining polarity, Cdc42-deficient DCs still managed to migrate toward an increasing concentration of CCL19, with only slightly reduced migration velocities as compared with wild-type DCs (see top panel of figure). The residual migratory capacity was likely due to largely intact Rac-induced spreading and lamellipodia formation. Thus, on 2-dimensional surfaces, Cdc42 was not absolutely required for directed cell motility. In a second set of experiments, Lämmermann et al examine the importance of Cdc42 during DC migration in geometrically more complex environments, that is, the 3-dimensional fibrillar networks of collagen matrices in vitro and dermis in vivo. Somewhat unexpectedly, DCs lacking Cdc42 were strongly impaired in their directed motility in 3-dimensional environments in vitro (see bottom panel of figure). Similarly, Cdc42-deficient DCs were entirely blocked in their migration from skin to draining PLNs, due to impaired entry into afferent lymphatic vessels. A more detailed morphologic analysis of Cdc42-deficient DCs uncovered that these cells became rapidly entangled within the 3-dimensional meshwork, with multiple protrusions pulling in different directions. Therefore, whereas migration efficiency in absence of Cdc42 was partially rescued due to the "lack of alternative routes" on 2-dimensional surfaces, cell motility in 3-dimensional environments absolutely required Cdc42. Although the function of Cdc42 in other leukocytes was not addressed in this study, recent studies provide solid evidence for a common integrin-independent, actin protrusion-dependent "amoeboid" migratory phenotype inside 3-dimensional environments in all hematopoietic cells.4–6 The data presented by Lämmermann et al support the notion that directional "decisiveness" conferred by Cdc42 is a critical element of this migration mode in complex 3-dimensional settings. The observations may also explain the reduced migration of DCs deficient in the Cdc42-effector Wiskott-Aldrich syndrome protein,7 although the more severe phenotype of Cdc42-deficient DCs reported by Lämmermann et al suggests the involvement of additional downstream effectors. Similarly, lymphocytes expressing a mutated form of the actin regulator Coronin1A, which results in excessive lamellipodia formation, show strongly impaired parenchymal motility.8 Together with this latest report from Lämmermann et al, these findings highlight the importance of tightly controlling actin cytoskeletal dynamics for efficient "decision-making" and maneuvering through complex 3-dimensional pore systems.
In conclusion, the authors demonstrate a requirement for Cdc42 in 3-dimensional environments to avoid "cellular trapping." Their findings also highlight the importance of choosing the appropriate experimental system—in particular, 2-dimensional versus 3-dimensional settings—to dissect the physiologic role of signaling molecules orchestrating cellular motility.
Footnotes
Conflict-of-interest disclosure: The author declares no competing financial interests.
REFERENCES
  CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
Related Article in Blood Online:
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 |
 |
 |
|||||||
 |
 |
 |
 |
 |
 |
 |
 |
||
| Copyright © 2009 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
