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Blood, 25 June 2009, Vol. 113, No. 26, pp. 6501-6502.
CEBPA resembles Roman god JanusERASMUS MC-SOPHIA CHILDREN'S HOSPITAL
In this issue of Blood, Ho and colleagues report the independent predictive value of single gene mutations in the CEBPA gene in pediatric acute myeloid leukemia. The outcome for this group of patients was excellent with 83% (± 13%) overall survival at 5 years from study entry.
In this issue, Ho et al screen a large series of pediatric AML samples, obtained from 3 Children's Oncology Group (COG) studies, for CEBPA mutations by fragment-length analysis.5 Acquired mutations were identified in 4.5% of all patients and clustered in CN-AML. In fact, 17% of pediatric patients with CN-AML were CEBPA-mutated. The majority of patients (82%) had "double" mutations in both the N-terminal domain and in the bZIP domain of the CEBPA gene. Patients with CEBPA-mutated AML showed similar survival rates as children with core-binding factor AML [ie, AML with inv(16) or t(8;21)]. No differences in outcome were detected between double and single mutants, which is in contrast to Wouters et al, who recently reported that favorable outcome is restricted to patients with "double mutations," which are usually localized on different alleles, resulting in the absence of wild-type protein.6 Recent data also suggest that accompanying genetic abnormalities may influence the prognostic impact of CEBPA mutations. For instance, Reneville et al showed that favorable outcome was restricted to patients with CN-AML without accompanying cytogenetic abnormalities or FLT3/ITD.7 Ho et al, however, found additional cytogenetic abnormalities in 14% of patients, with limited impact on outcome (overall survival 80% for patients with a normal karyotype, and 78% for all patients together). CEBPA, however, may contribute to leukemogenesis in opposite ways, which led us to compare CEBPA with the Roman god Janus. According to Roman mythology, he was the god of doors and gateways and is typically displayed as having 2 faces looking in opposite directions. His name is already permanently associated with the Janus kinases, which are characterized by an inhibitory and activating kinase domain and are involved in Down syndrome leukemias and T-cell acute lymphoblastic leukemia. At least 23 examples of "Janus-like" behavior of CEBPA need to be mentioned. For instance, apart from loss of function of the CEBPA protein by mutations or by epigenetic silencing (see below), overexpression of the CEBPA protein may also be leukemogenic, as reported by Chapiro et al.8 They showed that CEBPA is overexpressed due to a juxtaposition to the immunoglobulin gene promoter in patients with B-cell precursor acute lymphoblastic leukemia with a specific chromosomal translocation. Another intriguing example is the difference in outcome between patients with CEBPA silencing by mutations versus those with epigenetic silencing (5-year overall survival 88% vs 25%, respectively).9,10 This occurs despite the fact that both patient groups display marked similarity in gene expression signatures. However, the clinical phenotype of the leukemias with epigenetic CEBPA silencing is distinct, with expression of T-cell markers and NOTCH1 mutations.10 Moreover, Figueroa et al demonstrated that these samples were characterized by more widespread hypermethylation, which was not restricted to the CEBPA network only.9 These data now await confirmation by other adult AML groups and in pediatric cohorts, which are lacking to date. The next step is how to implement this knowledge into the clinic. Ho et al point out that, similar to patients with core-binding factor leukemias, patients with CEBPA-mutated AML should not be candidates for transplantation in first complete remission in future pediatric AML studies. This avoids the mortality and long-term morbidity with which this procedure is associated. Moreover, clinical studies need to be designed to assess whether the outcome of patients with epigenetic silencing can be improved by adding demethylating agents.
Footnotes
Conflict-of-interest disclosure: The authors declare no competing financial interests.
REFERENCES
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| Copyright © 2009 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
