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Blood, Vol. 101, Issue 6, 2167-2174, March 15, 2003
IL-7 surface-engineered lentiviral vectors promote survival and efficient gene transfer in resting primary T lymphocytes
Blood Verhoeyen et al.
101: 2167
Supplemental materials for: Verhoeyen et al, Vol 101, Issue 6, 2167-2174
Figure S1. HIV-1 derived vectors displaying human IL-7 share biologic properties with recombinant IL-7. Purified CD4+ naive (CD45RA) and memory (CD45RO) adult T cells and naive cord blood T cells were incubated with IL-7–displaying lentiviral vectors pseudotyped with VSV-G (G/IL7SU, G/IL7SUx) for 12 hours. IL-7R expression was then monitored with an anti-CD127 mAb (filled histograms). IL-7R expression was assessed in the 3 different T cells subsets incubated with VSV-G pseudotyped vectors (G) in the absence of rIL-7 (open histograms; isotype controls are presented by dashed-line histograms). Down-regulation of the IL-7R expression was compared with that observed in the presence of rIL-7 (G+rIL-7, filled histogram), and the specificity was demonstrated by incubating T cells with IL-2–displaying lentiviral vectors (G/IL2SU; filled histogram, upper row). Cell incubated only with culture medium show IL-7R expression equivalent to VSV-G vector incubation (only shown for CB cells, thick-line histogram, upper row). The FACS data presented are representative of 3 independent experiments.
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