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Blood, Vol. 103, Issue 11, 4302-4309, June 1, 2004
Human potassium chloride cotransporter 1 (SLC12A4) promoter is regulated by AP-2 and contains a functional downstream promoter element
Blood Zhou et al.
103: 4302
Supplemental materials for: Zhou et al, Vol 103, Issue 11, 4302-4309
Files in this Data Supplement:
- Table S1. Electrophoretic mobility shift oligonucleotide probes (PDF file, 40 KB)
- Figure S1. Mapping the 5' end of the human KCC1 cDNA (PDF file, 328 KB) -
Primer extension was carried out using 25 µg K562 total RNA as template. C, T, A, and G indicate nucleotide markers; lane 1, labeled primer plus K562 RNA; lane 2, labeled primer alone; and lane 3, labeled primer plus tRNA. The size of the extension products (lane 1, arrow) indicates that the 5' end of the mRNA is located 91 bp upstream relative to the 3' end of the primer. The cDNA sequence of this additional 5' untranslated cDNA was determined by 5' RACE and is shown in Figure 3.
- Figure S2. In vitro DNAse I foot printing of the human KCC1 gene promoter (PDF file, 44 KB) -
In vitro DNAse I footprinting of the human KCC1 gene promoter was performed using K562 extracts as described in the text. A single protected site was identified from −24 to −9 corresponding to AP-2 and Sp1 consensus binding sites.
- Figure S3. Downstream promoter sequences are critical for hKCC1 promoter function and contain a functional DPE (PDF file, 12KB) -
Plasmids containing hKCC1 gene promoter fragments inserted upstream of the firefly luciferase gene were transfected into K562 cells and luciferase activity was determined as described. Some hKCC1 promoter fragments contained truncations, insertions, or mutations (marked by an "X"). See for details. The data are means ± SDs of at least 6 independent transfection experiments.
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