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Blood, Vol. 102, Issue 5, 1849-1856, September 1, 2003
Identification of a gene expression signature associated with pediatric AML prognosis
Blood Yagi et al.
102: 1849
Supplemental materials for: Yagi et al, Vol 102, Issue 5, 1849-1856
Files in this Data Supplement:
- Table S1. Scaling factors used for expression value normalization (XLS file, 17 KB)
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Expression value normalization was carried out by multiplying raw expression values by these scaling factors so that the mean expression value in each experiment was 100.
- Table S2. The 135 prognosis-associated probe sets (133 genes) and their expression in the 54 patients (XLS file, 84 KB)
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Probe set IDs, gene symbols, and gene names of the 135 prognosis-associated probe sets are shown. Their expression in the 54 patients are also shown.
- Table S3. The 213 FAB subtype-specific probe sets (XLS file, 39 KB)
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Probe set IDs, gene symbols, and gene names of the 213 FAB subtype-specific probe sets are shown.
- Table S4. The chromosome rearrangement-specific probe sets (XLS file, 174 KB)
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Probe set IDs, gene symbols, and gene names of the 424 t(8;21)-specific, 471 inv(16)-specific, 144 t(9;11)-specific, and 388 11q23 rearrangement-specific probe sets are shown.
- Figure S1. An example of unsupervised 2-dimensional hierarchical clustering analysis of pediatric AML (JPG file, 292 KB)
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We first selected probe sets whose expression was significantly variable across the 54 pediatric AML patients from 12 566 probe sets on the microarray. In this analysis, we selected 749 probe sets whose median expression value in 54 pediatric AML patients was more than 20 and that showed more than 3-fold difference from the median in at least 5 patients. Then 2-dimensional hierarchical clustering analysis was carried out with these probe sets. Each column represents a patient, and each row represents a gene. The expression levels are normalized for each gene and indicated by color, with red representing high expression and green representing low expression. The dendrogram at the top shows the degree to which each patient is related to the others with respect to gene expression. Patients are marked according to their FAB subtypes and karyotypes.
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