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Blood, Vol. 102, Issue 7, 2581-2592, October 1, 2003
Gene profiling of a myeloma cell line reveals similarities and unique signatures among IL-6 response, N-ras-activating mutations, and coculture with bone marrow stromal cells
Blood Croonquist et al.
102: 2581
Supplemental materials for: Croonquist et al, Vol 102, Issue 7, 2581-2592
Data set 1: NIL: ANBL-6.plxsn in the absence of IL-6; A/MS: ANBL-6.plxsn cells after contact with MM bone marrow stromal cells; A/S: ANBL-6.plxsn cells after contact with normal bone marrow stromal cells; N-Ras: ANBL-6 stably transfected with mutant N-ras; IL-6: ANBL-6.plxsn cells stimulated with 10 ng/mL of recombinant IL-6.
Data set 2. To analyze genetic differences that distinguished and characterized the treatment groups uniquely, a class comparison tool BRB-Array Tools 2.0 (Dr Simon, National Cancer Institute) was employed which performs univariate F tests that are differentially expressed between 2 or more phenotype classes. This tool also computes a global permutation test that excludes genes that differ significantly due to chance alone. Genes that were significant (P < .001) after 2000 permutations were considered to be significantly differentially expressed.
Data set 3. Nonvariable genes among phenotypic classes were determined using a variance test (10% level) in combination with the parametric t test and nonparametric Kruskal-Wallis test (P < .001).
Files in this Data Supplement:
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