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Blood, Vol. 103, Issue 12, 4527-4535, June 15, 2004
The VEGF receptor flt-1 (VEGFR-1) is a positive modulator of vascular sprout formation and branching morphogenesis
Blood Kearney et al.
103: 4527
Supplemental materials for: Kearney et al, Vol 103, Issue 12, 4527-4535
Files in this Data Supplement:
- Movie S1. Mammalian sprouting angiogenesis and vessel fusion in differentiated embryonic stem (ES) cell cultures (MOV file, 1,851 KB)
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A 145-minute time-lapse video of a day-8 wild type (WT)–enhanced green fluorescent protein (eGFP) ES differentiation culture that illustrates the formation of vascular sprouts, sprout migration, and vessel fusion. Each frame corresponds to a single exposure; exposures were taken at one-minute intervals.
- Movie S2. Sprout formation in wild-type differentiated ES cultures (MOV file, 3,887 KB)
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A 10-hour time-lapse video of a day-8 WT-eGFP ES differentiation culture that shows numerous new wild-type sprouts forming throughout the vascular network. These sprouts either remain extended or fuse with other sprouts or primary vessels. Each frame corresponds to a single exposure; exposures were taken at one-minute intervals.
- Movie S3. flt-1-/--differentiated ES cultures exhibit decreased levels of new sprout formation (MOV file, 4,074 KB)
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A 10-hour time-lapse video of day-8 flt-1-/--eGFP ES differentiation culture that illustrates typical levels of sprout formation in flt-1-/- vessels. Notice how very few flt-1-/- sprouts form relative to the wild-type culture (Movie S2). Each frame corresponds to a single exposure; exposures were taken at one-minute intervals.
- Movie S4. Wild-type sprout velocity (MOV file, 626 KB)
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Over a 40-minute period, a wild-type sprout from a day-8 WT-eGFP ES differentiation culture forms and migrates a distance of 40 µm. Each frame corresponds to a single exposure; exposures were taken at one-minute intervals.
- Movie S5. flt-1-/- sprouts exhibit significantly decreased sprout velocities (MOV file, 925 KB)
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Over a 100-minute period, a flt-1-/- mutant sprout from a day-8 flt-1-/--eGFP ES differentiation culture forms and migrates a distance of 20 µm. Slower sprout velocities (relative to wild-type controls) were typical of flt-1-/- sprouts (see the corresponding graph in Figure 2D). Each frame corresponds to a single exposure; exposures were taken at one-minute intervals.
- Movie S6. Endothelial cell division during wild-type vascular development (MOV file, 3,541 KB)
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A 278-minute time-lapse video of day-6 WT-eGFP ES differentiation culture displaying typical levels of endothelial cell division in day-6 wild-type cultures. Note that over a similar time span there are more divisions scored in the day-6 flt-1-/- mutant vessels than in wild-type controls (see Movie S7 and the graph in Figure 5C). Each frame corresponds to a single exposure; exposures were taken at one-minute intervals.
- Movie S7. flt-1-/- ES cultures exhibit elevated levels of endothelial cell division at day 6 of differentiation (MOV file, 3,915 KB)
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A 216-minute time-lapse video of day-6 flt-1-/--eGFP differentiation culture that shows numerous flt-1-/- endothelial cells undergoing cell division. Note the increased number of endothelial cell divisions compared to wild-type (see Movie S6 and the graph in Figure 5C). Each frame corresponds to a single exposure; exposures were taken at one-minute intervals.
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