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Blood, Vol. 103, Issue 10, 3828-3836, May 15, 2004
Immunoglobulin class-switch recombination in mice devoid of any Sµ tandem repeat
Blood Khamlichi et al.
103: 3828
Supplemental materials for: Khamlichi et al, Vol 103, Issue 10, 3828-3836
Files in this Data Supplement:
- Figure S1. Analysis of early B-cell development by flow cytometry (PDF, 53 KB) -
Bone marrow cells from 6- to 8-week-old wt or
 / mice were labeled with Spectral Red–conjugated anti-B220 and phycoerythrin-conjugated anti–c-kit, anti-CD25, anti-IgM, or anti-IgD. The results shown are representative of four independent experiments.
- Figure S2. Nucleotide sequence of the region encompassing the proximal part of the core Sµ (PDF, 40 KB) -
The altered nucleotides are underlined and the replacing nucleotides are shown below in bold lowercase letters. The HindIII site at the 5’ end of the core Sµ is shown in bold.
- Figure S3. Nucleotide sequence of CSR junctions in Iµ-Cµ intron-deleted mice (PDF, 140 KB) -
Group A comprises the junctions within Iµ, and group B, those within the mutated Iµ-Cµ intron. (Top) sequence of the wild type Iµ. (Middle) sequence of the wild type S
 3. (Bottom) sequence of the junction (Iµ sequences in capital letters and S3 sequences in lowercase letters). The asterisks denote identity between the wild-type and the recombined S3 sequences; the mutations are indicated by empty spaces. The dashes indicate microhomology within the 10 nucleotides flanking the breakpoints (framed by a rectangle). The mutated nucleotides in Iµ are shown in gray and underlined (in TS19 from group A and in TS17 from group B). TS17 represents the clone in which the junction took place in the loxP site, the EcoRI site just upstream from the loxP site (Figure 1) is underlined.
- Figure S4. Computer-assisted search for secondary structures in the mutated Iµ-Cµ region (PDF, 151 KB) -
The program and the folding conditions were as described.49 The breakpoints are indicated by an arrow.
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