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Blood, Vol. 103, Issue 8, 2981-2989, April 15, 2004
CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow
Blood Avigdor et al.
103: 2981
Supplemental materials for: Avigdor et al, Vol 103, Issue 8, 2981-2989
Files in this Data Supplement:
- Video 1. Random movement of CB CD34+ cells on HA in the absence of SDF-1 (AVI file, 2.49 MB)
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Enriched CB CD34+ cells were plated on HA-coated cover slips and allowed to adhere for 30 minutes. Cell movement was recorded using a scientific-grade CCD camera. Frames were taken every 2 seconds. Original magnification, ×160. The original speed is increased about 6-fold in the video.
- Video 2. Movement of CB CD34+ cells on HA in the presence of SDF-1 gradient (AVI file, 2.75 MB)
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Enriched CB CD34+ cells were plated on HA-coated cover slips and allowed to adhere for 30 minutes. Cell movement was recorded using a scientific-grade CCD camera. A gradient of SDF-1 was established by an application of SDF-1–soaked agarose beads, which were preincubated for 1 hour with recombinant SDF-1 solution. The chemokine gradient over a well was established 5 to 10 minutes following the introduction of the beads. Frames were taken every 2 seconds. Note the highly polarized phenotype and the multiple pseudopodia protruded at the direction of cell movement towards SDF-1 source. Original magnification, ×160. The original speed is increased about 6-fold in the video.
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