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Blood, Vol. 103, Issue 10, 3890-3896, May 15, 2004
TEL-AML1 promotes development of specific hematopoietic lineages consistent with preleukemic activity
Blood Morrow et al.
103: 3890
Supplemental materials for: Morrow et al, Vol 103, Issue 10, 3890-3896
Files in this Data Supplement:
- Table S1. Response of a TEL-AML1 expressing pre-B cell line to recombinant growth factors (JPG, 24.8 KB)
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Cells were seeded in 96-well plates (5 x 104/100µL) in basic medium containing various combinations of IL-7 (20 ng/mL), Flt3-L (10 ng/mL), and SCF (100 ng/mL). Proliferation was measured 3 days later using the CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega) and measuring absorbance at 490 nm. Bars represent the fold increase in absorbance of cells cultured with growth factors over cells cultured in medium alone. The mean and standard deviation of triplicate cultures are shown. These data are representative of 3 independent experiments and similar data were obtained with 3 different pre-B cell lines induced by TEL-AML1.
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