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Blood, Vol. 103, Issue 12, 4432-4439, June 15, 2004
Modulating erythrocyte chimerism in a mouse model of pyruvate kinase deficiency
Blood Richard et al.
103: 4432
Supplemental materials for: Richard et al, Vol 103, Issue 12, 4432-4439
Files in this Data Supplement:
- Table S1. Hematologic analysis of long-lived, chemical inducers of dimerization (CID)–treated transplanted mice (PDF, 102 KB) -
Three CBA/N (4895, 4896, 4897) and 3 CBA-Pk-1slc/Pk-1slc (4202, 4203, 4204) mice were sacrificed at the same time as the 6 experimental mice. Mice were evaluated for red cell number, percentage of GFP + RBCs, spleen size, leukocyte differential on a peripheral smear, and myeloid-to-erythroid ratio on a smear of bone marrow (BM M:E).
* Mouse 2411 had a significant number of nucleated erythrocytes and erythroid precursors (Nuc. RBCs) on the peripheral blood smear.
# Mouse 2407 had a large percentage of lymphocytes on the peripheral blood smear that were also present in the bone marrow.
- Figure S1. Pathologic evaluation of the bone marrow of long-lived, CID-treated, transplanted mice (JPG, 116 KB)
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Femurs from the 6 experimental mice (see Table S1), 1 CBA/N mouse, and 1 CBA-Pk-1slc/Pk-1slc mouse were fixed and stained with hematoxylin/eosin. Mouse 2407 had less than 10% of the marrow space with normal-appearing hematopoiesis, with more than 90% appearing aplastic. The CBA-Pk-1slc/Pk-1slc mouse and the transplanted experimental mice had normal-appearing bone marrow with elevated erythropoietic elements. The bone marrow from the CBA/N mouse was normal. Original magnification x20.
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