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Blood, Vol. 104, Issue 7, 2010-2019, October 1, 2004
The role of angiopoietins in the development of endothelial cells from cord blood CD34+ progenitors
Blood Hildbrand et al.
104: 2010
Supplemental materials for: Hildbrand et al, Vol 104, Issue 7, 2010-2019
Files in this Data Supplement:
- Figure S1. (JPG, 97 KB)
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Top panel: RT-PCR analysis of KDR transcripts expressed in HUVECs and CD34+ cells purified from 2 cord-blood donors (CB-1 and CB-2). KDR-specific transcripts are not detectable in mRNA preparation from freshly isolated cord-blood CD34+ cells (day 0) but are readily detectable in the same CD34+ cells cultured for 2 days, as well as in HUVECs. KDR specific primers were: 5'-CTGGAGCCTACAA-GTGCTTCTACC-3' (forward) and 5'-TGGACCCTGACAAATGTGCTG-3' (reverse). The bottom panel shows the PCR product resulting from the amplification of GAPDH from the same mRNA preparations, to control for similar amounts of mRNA template run in the PCR reactions.
- Figure S2. (JPG, 693 KB)
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Representative photomicrographs of individual endothelial cell colonies developed at day 7 of culture (left panels). These colonies, when plucked and replated, expanded into fully confluent endothelial cell monolayers at day 21 of culture (right panels).
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