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Blood, Vol. 103, Issue 11, 4207-4215, June 1, 2004
Infection of mature monocyte-derived dendritic cells with human cytomegalovirus inhibits stimulation of T-cell proliferation via the release of soluble CD83
Blood Sénéchal et al.
103: 4207
Supplemental materials for: Sénéchal et al, Vol 103, Issue 11, 4207-4215
Files in this Data Supplement:
- Figure S1. IE1+ immature moDCs are resistant to exogenous maturation stimuli (PDF file, 60 KB) -
HCMV infection inhibited the response of IE1+ immature moDCs to exogenous maturation stimuli. IL-1-beta, IL-6, TNF-alpha, and PGE2 were added 24 hours after infection of immature moDCs, and phenotype was assessed 2 days later (day +3 after infection). IE1+ moDCs from HCMV-infected cultures did not appropriately increase expression of class I and II MHC, CD86, CD80, CD83, or CD25 in response to maturation stimuli (middle row). This was in contrast to the up-regulation of these epitopes by bystander IE1– cells (bottom row) or noninfected controls (top row). The x-axis depicts log10 fluorescence, and the y-axis depicts cell frequency. The numbers in each panel correspond to the mean fluorescent intensity of the population for the indicated epitope.
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