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Blood, Vol. 103, Issue 12, 4496-4502, June 15, 2004
Human marrow stromal cells activate monocytes to secrete osteopontin, which down-regulates Notch1 gene expression in CD34+ cells
Blood Iwata et al.
103: 4496
Supplemental materials for: Iwata et al, Vol 103, Issue 12, 4496-4502
Files in this Data Supplement:
- Document 1. Abstract of experiments (PDF file, 52 KB) -
Abstract of experiments comparing gene expression of cocultured monocytes and stromal cells to stromal cells.
- Document 2. Methods used to determine gene expression ratios for HS-27a culture and HS-27a–monocyte coculture (PDF file, 60 KB)
- Document 3. Basic description of sample and microarray analysis parameters according to Minimal Information About a Microarray Experiment (MIAME) guidelines (TXT file, 12 KB)
- Table S1. Sample equivalence table for association of raw data file names with summary tables and GEO accession numbers (XLS file, 16 KB)
- Data Set 1A. (TXT file, 1.97 MB)
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Part of Data Set 1. Raw data from GenePix Pro scan of 7 microarray experiments. There are six more below.
- Data Set 1B (TXT file, 1.96 MB)
- Data Set 1C (TXT file, 1.95 MB)
- Data Set 1D (TXT file, 1.94 MB)
- Data Set 1E (TXT file, 1.93 MB)
- Data Set 1F (TXT file, 1.96 MB)
- Data Set 1G (TXT file, 1.91 MB)
- Data Set 2. GenBank accession numbers and Unigene IDs (TXT file, 1.2 MB)
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Merge file to be used to associate GenBank accession numbers and Unigene IDs with spots on the microarrays.
- Data Set 3. Stromal cell cultures and stromal-monocyte cocultures (TXT file, 6.35 MB)
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CyberT input file, with lowess normalized and ln-converted ratios of stromal-monocyte cocultures to stromal cells cultured alone. Cocultures are in the F532 channel, and HS-27a alone is in the F635 channel.
- Data Set 4. Significant genes (TXT file, 16 KB)
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CyberT output listing significant genes before removal of those identified as high in monocytes. The last column gives approximate fold difference in gene expression between CD14+ cells and HS-27a stromal cells.
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