|
|
Blood, Vol. 104, Issue 5, 1411-1418, September 1, 2004
Antineutrophil cytoplasmic autoantibodies against the murine homolog of proteinase 3 (Wegener autoantigen) are pathogenic in vivo
Blood Pfister et al.
104: 1411
Supplemental materials for: Pfister et al, Vol 104, Issue 5, 1411-1418
Files in this Data Supplement:
- Figure S1. Schematic illustration of the histomorphometric analysis of skin lesions (PDF file, 132 KB) -
Both biopsies from each animal were cut (slice thickness 5 µm, left panel) into serial slices. Slices spanning the region of TNF-
 injection were digitalized in 45- to 70-µm intervals using a microscope equipped with a digital camera. Image analysis was performed with digital image analysis software (KS 300; Carl Zeiss, Jena, Germany).
The outer perimeter of the inflamed area from each tissue section was specified manually in a blinded fashion. Blood vessels were marked and manually exluded; fat vacuoles of adipocytes were not covered by the image analysis program. The region specified in this way was overlaid with a black mask, which is illustrated by the right upper and lower panels, respectively. The area of the mask was determined for several slices. Peak values from 5 to 8 consecutive slices that covered a layer thickness of 300 µm of the biopsy in total were averaged.
|
|
