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Blood, Vol. 105, Issue 2, 609-616, January 15, 2005
Competitive clonal hematopoiesis in mouse chimeras explained by a stochastic model of stem cell organization
Blood Roeder et al.
105: 609
Supplemental materials for: Roeder et al, Vol 105 Issue 2, 609-616
Files in this Data Supplement:
- Table S1. Model parameters for the different simulation scenarios. (PDF, 84KB) -
B6 reference values are based on previous simulation studies15. Instead of using variable generation times ( c) ranging from 12 to 96 hours as in the above cited publication, we chose fixed, but strain specific cD2/B6. Furthermore, the differentiation coefficient (d) has changed for technical reasons from 1.04 to 1.07. Neither change alters the general system behavior.
D2 values have been obtained by fitting the shape parameters of the transition characteristics f and f to the data set shown in Figure 3A and 5B, respectively. In case of the simulation of cytokine administration, f is assumed to be constant, i.e. the function values at cell numbers 0, N/2, N, and infinity in GE- , are fixed at the given value.
- Figure S1. Growth environment transition intensities. (PDF, 11 KB) -
(A) The intensity, i.e. the probability per time step  t (here set to 1 hour), of a stem cell to change form GE- to GE-A, denoted by , is defined as the product of the transition characteristic f and a / amax, with a representing the actual affinity of the cell and amax=1. (B) Similarly, the intensity to change from GE-A to GE-, denoted by , is defined as the product of transition characteristic f and amin / a, with amin=0.01. Both transition characteristics are uniquely specified by 5 parameters: the reference cell number N (scaling factor), the limiting behaviors (function values at cell numbers 0 and infinity), and the shape parameters (function values at cell numbers N/2 and N). If cells do not undertake a transitions from one GE to the other in t, which is occurring with probabilities 1- and 1- for GE- and GE-A, respectively, they stay inside the actual growth-environment and the affinity a is changed according to the following rules:
GE-:
GE-A:
- Figure S2. Simulation results on the effects of quantitative differences in transition characteristics on chimerism development. (PDF, 37 KB) -
(A) Shown are 3 competition scenarios which differ with respect to the used transition characteristics. The 3 average simulations show the chimerism development in primary chimeras after initialization with 20 stem cells and the reconstitution of these systems after a kill of 95% of all model cells. Herein, the B6 parameters are fixed for all 3 scenarios, whereas the D2 characteristics are varied as indicated in panels B and C, respectively. The inline figures (in panel B, C) are zooming the regulatory region underlying the chimerism development in fully reconstituted animals. These differences are responsible for observed long-term trends in the chimerism pattern.
- Equation for GE- for Figure S1
- Equation for GE-A for Figure S1
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