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Blood, Vol. 104, Issue 6, 1801-1807, September 15, 2004
Fc RIII discriminates between 2 subsets of V9V 2 effector cells with different responses and activation pathways
Blood Angelini et al.
104: 1801
Supplemental materials for: Angelini et al, Vol 104, Issue 6, 1801-1807
Files in this Data Supplement:
- Figure S1. Vδ2+ TEMh1 clones, but not Vδ2+ TEMRA clones, downregulate chemokine receptors following antigenic stimulation (PDF, 51KB) -
Clones were generated by single-cell sorting from PBLs stained with Vδ2, CD45RA, CD16, CD62L, and CD27. Cells were cultured as previously described.1 Expression levels of chemokine receptors by Vδ2 T-cell clones following 24 hours of stimulation with 10 nM BrHPP and 50 U/ml IL-2 were evaluated with flow cytometry.
- Figure S2. Different functional responses of Vd2TEMh and Vd2TEMRA T-cell clones (PDF, 56 KB) -
The Vδ2high and Vδ2low phenotypes are stable in culture and correspond to TEMh and TEMRA subsets, respectively. Vδ2 T-cell clones were established following single-cell sorting based on TCR Vδ2 expression levels. The phenotypes were monitored in culture, and clones were stimulated with 20 nM BrHPP at 37°C at three time points.
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