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Blood, Vol. 105, Issue 4, 1431-1439, February 15, 2005 This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef Induction of T-cell development from human cord blood hematopoietic stem cells by Delta-like 1 in vitro Blood La Motte-Mohs et al. 105: 1431 Supplemental materials for: La Motte-Mohs et al., Vol 105, Issue 4, 1431-1439 Files in this Data Supplement: Figure S1 (JPG, 150 KB) - Induction of Notch signaling following coculture of human cord-blood derived HSCs on OP9-control and OP9-DL1 stromal cells: CD34+CD38- cells sorted from human cord blood were cocultured with OP9-control cells or OP9-DL1 cells for 40 days. At day 40 of coculture, mRNA was isolated and reverse transcripted into cDNA. RT-PCR was carried out to determine the expression of human Deltex-1 and of HES-1 at three different dilutions of cDNA. OP9-control and OP9-DL1 cells did not express human Deltex-1 or HES-1 (data not shown). Both cocultures were normalized to the detectable levels of -actin expression. Figure S2 (JPG, 95 KB) - Flow cytometric analysis of CD45 and CD19 expression on differentiating human cord blood-HSCs following long-term coculture with OP9-control cells: CD34+CD38- cells sorted from human cord blood were cocultured with OP9-control stromal cells for 36 days. Induction of the B-cell phenotype was evaluated approximately every 4 days of coculture through the coexpression of CD45 and CD19. Shown are selected time points during the coculture. Figure S3 (JPG, 95 KB) - Diverse V gene expression on cord blood–derived CD3+TCR+ cells following long-term coculture with OP9-DL1 cells: CD34+CD38- cells sorted from human cord blood were cocultured with OP9-DL1 cells for 40 days. (A) Expression of TCR on CD3+-gated cells was determined by flow cytometric analysis and is presented in the form of a histogram. (B) Expression of several TCR-V genes was determined by flow cytometric analysis and is presented in the form of a histogram. The expression of V3 and V8 on CD3+-gated cells is shown and compared with one of several isotype (IgG2a) controls (see "Materials and methods"). (C) The use of several V genes was determined on CD3+-gated cells by dividing the percent of V+ cells by the percent of TCR+ cells on CD3+ cells. This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Rights and Permissions Citing Articles Citing Articles via CrossRef

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