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Blood, Vol. 104, Issue 10, 3106-3116, November 15, 2004
Differences in the chromatin structure and cis-element organization of the human and mouse GATA1 loci: implications for cis-element identification
Blood Valverde-Garduno et al.
104: 3106
Supplemental materials for: Valverde-Garduno et al, Vol 104, Issue 10, 3106-3116
Files in this Data Supplement:
- Table S1. DNase I Hypersensitive sites in the mouse (a) and human (b) GATA1 loci (PDF, 132 KB)
- Figure S1: Acetylation profile of histone H4 in the mGata1 locus (PDF, 25 KB) -
The mGata1 locus is shown at the top of the figure. Genes are depicted as black boxes, and the positions of haematopoietic DNase I HSs (gray arrows) and widely expressed DNase I HSs (black arrows) are shown below the locus.
Below the locus, panels show the profile of acetylated H4 at different points in the mGata1 locus, as determined by chromatin immunoprecipitation (ChiP) using real-time Taqman PCR analysis in an erythroid cell line (MEL), megakaryocytic cell line (L8057), primary eosinophils (Io eosinophils), and a fibroblast cell line (3T3 cells). The fold enrichment of acetylated H4 (y-axis) is shown at different points along the mGata1 locus (x-axis). The coordinates are on kilobases with respect to the transcriptional start site of the mGata1 IE promoter. The black bars show average fold enrichment results from duplicate real-time PCR reactions performed on 3 independent ChiP preparations. Error bars indicate standard deviation.
- Figure S2: Acetylation profile of histone H4 in the hGATA1 locus (PDF, 22 KB) -
The figure is organized in the same as Figure S1: the hGATA1 locus is shown at the top of the figure, and the panels below the locus show the profile of acetylated H4 at different points in the hGATA1 locus in primary erythroid cells (Io erythroid), primary eosinophils (Io eosinophils), and fibroblast cell line (HeLa). Data shown are average fold enrichments results from duplicate real-time PCR reactions performed on 3 independent ChiP preparations. Error bars indicate standard deviation.
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